چهارشنبه 24 اردیبهشت 1404
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دوره 19، شماره 5 - ( 3-1400 )
جلد 19 شماره 5 صفحات 172-172 |
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Naseri L, Khazaei MR, Khazaei M. P-5 Potential therapeutic effect of bee pollen and metformin combination on testosterone and estradiol levels, apoptotic markers and total antioxidant capacity in a rat model of polycystic ovary syndrome. IJRM 2021; 19 (5) :172-172
URL: http://ijrm.ir/article-1-2862-fa.html
URL: http://ijrm.ir/article-1-2862-fa.html
P-5 Potential therapeutic effect of bee pollen and metformin combination on testosterone and estradiol levels, apoptotic markers and total antioxidant capacity in a rat model of polycystic ovary syndrome. International Journal of Reproductive BioMedicine. 1400; 19 (5) :172-172
چکیده: (284 مشاهده)
Background: Polycystic ovary syndrome (PCOS) is associated with metabolic disorders as well as infertility. Many traditional remedies have been reported to show estrogenic and antioxidant potential. Bee pollen (BP) is a natural compound, reported as one such remedy.
Objective: The present study aimed to investigate the effects of BP extract (BP) and metformin (MET) on estradiol (E2) and testosterone (T) levels, apoptotic markers, and total antioxidant capacity (TAC) in a rat model of PCOS.
Materials and Methods: In this experimental study, 54 female Wistar (n = 6/group) rats received 2 mg of estradiol valerate (EV) intramuscularly and 6 additional rats were considered the control without EV injection. The rats were treated with BP (50, 100, and 200 mg/kg), MET (300 mg/kg) and BP+MET (50 BP+300 MET, 100 BP+300 MET, and 200 BP+300 MET mg/kg). Serum levels of E2 and T were assessed by the ELISA method. TAC of serum was also determined. The expressions of Bcl2, Bax, Caspase3 (Cas-3), and Sirt-1 genes were evaluated by real-time polymerase chain reaction (PCR). Data were statistically analyzed using one-way ANOVA.
Results: In the untreated PCOS group E2 and T levels (p < 0.01), and Bcl2 (p = 0.007) expression were increased, but TAC (p = 0.002) and expression of Bax (p = 0.001), Cas-3 and Sirt-1 (p < 0.01) were decreased significantly. The levels of E2 and T, as well as the expressions of Bcl2, were decreased in all treated groups compared to the untreated PCOS group (p < 0.01). On the other hand, TAC and expression of Bax, Cas-3and Sirt-1 were increased in the BP- and MET-treated groups (p < 0.05).
Conclusion: BP and MET synergistically improved serum E2, T, and TAC levels, and expression of apoptotic genes.
Objective: The present study aimed to investigate the effects of BP extract (BP) and metformin (MET) on estradiol (E2) and testosterone (T) levels, apoptotic markers, and total antioxidant capacity (TAC) in a rat model of PCOS.
Materials and Methods: In this experimental study, 54 female Wistar (n = 6/group) rats received 2 mg of estradiol valerate (EV) intramuscularly and 6 additional rats were considered the control without EV injection. The rats were treated with BP (50, 100, and 200 mg/kg), MET (300 mg/kg) and BP+MET (50 BP+300 MET, 100 BP+300 MET, and 200 BP+300 MET mg/kg). Serum levels of E2 and T were assessed by the ELISA method. TAC of serum was also determined. The expressions of Bcl2, Bax, Caspase3 (Cas-3), and Sirt-1 genes were evaluated by real-time polymerase chain reaction (PCR). Data were statistically analyzed using one-way ANOVA.
Results: In the untreated PCOS group E2 and T levels (p < 0.01), and Bcl2 (p = 0.007) expression were increased, but TAC (p = 0.002) and expression of Bax (p = 0.001), Cas-3 and Sirt-1 (p < 0.01) were decreased significantly. The levels of E2 and T, as well as the expressions of Bcl2, were decreased in all treated groups compared to the untreated PCOS group (p < 0.01). On the other hand, TAC and expression of Bax, Cas-3and Sirt-1 were increased in the BP- and MET-treated groups (p < 0.05).
Conclusion: BP and MET synergistically improved serum E2, T, and TAC levels, and expression of apoptotic genes.
نوع مطالعه: Congress Abstract |
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