International Journal of
Reproductive Biomedicine
Wed, May 14, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 237-237 |
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Khodabandeh Z, Dolati P, Zamiri MJ, Mehrabani D, Bordbar H, Alaee S, et al . P-70 The stereological evaluation of testis structure on protective effect of quercetin against lead acetate toxicity. IJRM 2021; 19 (5) :237-237
URL: http://ijrm.ir/article-1-2871-en.html
URL: http://ijrm.ir/article-1-2871-en.html
Khodabandeh Z *1 
, Dolati P2 , Zamiri MJ3 , Mehrabani D4 , Bordbar H5 , Alaee S6 , Jamhiri I7 , Azarpira N8
, Dolati P2 , Zamiri MJ3 , Mehrabani D4 , Bordbar H5 , Alaee S6 , Jamhiri I7 , Azarpira N8
1- Stem Cells Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran. , Jahromi@sums.ac.ir
2- Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran.
3- Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
4- Stem Cells Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran. Li Ka Shing Center for Health Research and Innovation, University of Alberta, Edmonton, AB, Canada.
5- Histomorphometry and Stereology Research Center, Shiraz University of Medical Science, Shiraz, Iran. Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
6- Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
7- Stem Cells Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran.
8- Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran.
3- Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
4- Stem Cells Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran. Li Ka Shing Center for Health Research and Innovation, University of Alberta, Edmonton, AB, Canada.
5- Histomorphometry and Stereology Research Center, Shiraz University of Medical Science, Shiraz, Iran. Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
6- Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
7- Stem Cells Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran.
8- Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract: (254 Views)
Background: Exposure to environmental pollutants tightly impacts on male fertility sometimes are irretrievable.
Objective: In the present study, we studied the toxic effects of lead acetate (Pb) on testicular structure, and the possible effect of quercetin on qualifying these effects.
Materials and Methods: Experimental groups, including the Pb, quercetin (QE), (Pb + QE), and control mice, were treated at least one spermatogenic cycle. The fixed testes were dehydrated in graded ethanol, cleared in xylene, and embedded in paraffin wax. Serial sections were prepared using Cavalieri method in a series of equal parallel planes (5 and 20-μm thickness).Then the samples were evaluated by stereological methods.
Results: Testicular weight, both absolute and relative, was higher in Pb-exposed mice in comparison with the control and Pb-quercetin groups. The increase in the size of testis was related to the lumen and connective tissue in this group. Lead acetate induced different patterns in testicular cell number; as spermatogonia, spermatocyte, and Sertoli cells number did not affect in lead acetate exposed group, while the total number of round spermatids and long spermatids significantly reduced.
Conclusion: In conclusion, Pb administration adversely impacted on the cellular organization and activation of the apoptotic pathways in the testis; on the other hand, quercetin co-administration with lead partially ameliorated these adverse effects.
Objective: In the present study, we studied the toxic effects of lead acetate (Pb) on testicular structure, and the possible effect of quercetin on qualifying these effects.
Materials and Methods: Experimental groups, including the Pb, quercetin (QE), (Pb + QE), and control mice, were treated at least one spermatogenic cycle. The fixed testes were dehydrated in graded ethanol, cleared in xylene, and embedded in paraffin wax. Serial sections were prepared using Cavalieri method in a series of equal parallel planes (5 and 20-μm thickness).Then the samples were evaluated by stereological methods.
Results: Testicular weight, both absolute and relative, was higher in Pb-exposed mice in comparison with the control and Pb-quercetin groups. The increase in the size of testis was related to the lumen and connective tissue in this group. Lead acetate induced different patterns in testicular cell number; as spermatogonia, spermatocyte, and Sertoli cells number did not affect in lead acetate exposed group, while the total number of round spermatids and long spermatids significantly reduced.
Conclusion: In conclusion, Pb administration adversely impacted on the cellular organization and activation of the apoptotic pathways in the testis; on the other hand, quercetin co-administration with lead partially ameliorated these adverse effects.
Type of Study: Congress Abstract |
Subject:
Pregnancy Health
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