International Journal of
Reproductive Biomedicine
Tue, May 13, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 242-242 |
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Hemati M, Nikukar H, Sadeghian-Nodoushan F, Abdoli AM, Haghirosadat BF. P-75 Development and characterization of a novel PEGylated liposome-encapsulated Ceratonia siliqua to improved sperm parameters. IJRM 2021; 19 (5) :242-242
URL: http://ijrm.ir/article-1-2876-en.html
URL: http://ijrm.ir/article-1-2876-en.html
1- Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Research and Clinical Centre for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,fhaghirosadat@gmail.com
2- Research and Clinical Centre for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,
Abstract: (229 Views)
Background: Ceratonia silique is found to have antioxidative properties that may inactivate free radicals and minimize the oxidative stress inside the testis cells in infertile male. Also, it is reported as a protective pharmacological agent against several diseases. In order to increase the drug stability, bioavailability, and to deliver the drug in the targeted tissue, it can be incorporated in a nano-sized vesicle of liposome formulations.
Objective: The aim of this study was to optimize the PEGylated liposome-containing carob to enhance the therapeutic response.
Materials and Methods: Polyethylene glycol (Lipoid PE 18:0/18:0-PEG2000, DSPE-mPEG 2000) and 1, 2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Lipoid GmbH (Ludwigshafen, Germany). Cholesterol and all chemicals, solvents, and salts were bought from Sigma Chemical Company in St. Louis: USA. In this study, we synthesized liposome formulations containing cholesterol: DPPC: DSPE-PEG2000 at a molar ratio of 70:30:5 and thin-film evaporation method was used for the preparation of stealth controlled- release liposomal the carob. Outcome parameters were mean size of the vesicle, zeta potential, entrapment efficiency and in vitro drug release.
Results: The formulation of liposomal carob demonstrated that the optimum nano-vesicle size with an average diameter of below 100 nm. The vesicles have a suitable negative charge that are stable without aggregation at 4°C. The entrapment efficiency (EE%) for carob was above 80%. Moreover, the release profile of carob during 72 h was slow and continuous with an initial rapid release period followed by a slower release phase.
Conclusion: In conclusion, we successfully developed a stealth liposomal carob with a high potential for systemic delivery. The sustained-release properties of liposomal carob as a successful lipid-based nano-carriers that improves in vivo stability of the drug and leads to pharmaceutical benefits increase and loss of the drugs followed by the high-dose drug.
Objective: The aim of this study was to optimize the PEGylated liposome-containing carob to enhance the therapeutic response.
Materials and Methods: Polyethylene glycol (Lipoid PE 18:0/18:0-PEG2000, DSPE-mPEG 2000) and 1, 2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Lipoid GmbH (Ludwigshafen, Germany). Cholesterol and all chemicals, solvents, and salts were bought from Sigma Chemical Company in St. Louis: USA. In this study, we synthesized liposome formulations containing cholesterol: DPPC: DSPE-PEG2000 at a molar ratio of 70:30:5 and thin-film evaporation method was used for the preparation of stealth controlled- release liposomal the carob. Outcome parameters were mean size of the vesicle, zeta potential, entrapment efficiency and in vitro drug release.
Results: The formulation of liposomal carob demonstrated that the optimum nano-vesicle size with an average diameter of below 100 nm. The vesicles have a suitable negative charge that are stable without aggregation at 4°C. The entrapment efficiency (EE%) for carob was above 80%. Moreover, the release profile of carob during 72 h was slow and continuous with an initial rapid release period followed by a slower release phase.
Conclusion: In conclusion, we successfully developed a stealth liposomal carob with a high potential for systemic delivery. The sustained-release properties of liposomal carob as a successful lipid-based nano-carriers that improves in vivo stability of the drug and leads to pharmaceutical benefits increase and loss of the drugs followed by the high-dose drug.
Type of Study: Congress Abstract |
Subject:
Pregnancy Health
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