International Journal of
Reproductive Biomedicine
Wed, May 14, 2025
[Archive]
Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 175-175 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Sadeghi Z, Honarvar A, Dashti G. P-8 Effects of nicotinic acid and Folic acid on sperm motility, Viability and DNA integrity in oligospermia men during cryopreservation. IJRM 2021; 19 (5) :175-175
URL: http://ijrm.ir/article-1-2879-en.html
URL: http://ijrm.ir/article-1-2879-en.html
1- Department of Anatomy, Isfahan University of Medical Sciences, Isfahan, Iran.
2- Department of Anatomy, Isfahan University of Medical Sciences, Isfahan, Iran. ,dashti@med.mui.ac.ir
2- Department of Anatomy, Isfahan University of Medical Sciences, Isfahan, Iran. ,
Abstract: (302 Views)
Background: Sperm freezing is an important technique for treating infertility and maintaining sperm, but because of the increased production of reactive oxygen species (ROS), threats such as chromatin damage and sperm DNA, threaten sperm motility and consequently reduce fertility. Antioxidants are important compounds to minimize the deleterious effects of freezing sperm and maintaining fertility potential.
Objective: The aim of this study was to investigate the effect of nicotinic acid and folic acid antioxidants on the sperm motility, survival and deoxyribonucleic acid of oligospermia men during cryopreservation.
Materials and Methods: For this purpose, 25 semen samples of Oligospermia men who referred to the Fertility and Infertility Center of Shahid Beheshti Hospital in Isfahan province were randomly taken into sterile containers and after fluidization, sperm parameters including morphology, motility, and concentration, life and quality of chromatin and their DNA were Measure according to WHO criteria. Then, each sample was divided into 4 parts with freeze-dried medium for freezing: antioxidant-free (control group), 50 nM folic acid, 10 mM nicotinic acid, 50 nM folic acid + 10 mM nicotinic acid. After freezing, the samples were thawed and reexamined for sperm parameters.
Results: Our study showed that the process of freezing sperm by damaging the spermatozoa and cell membrane, resulted in decreased motility and competence of sperm to fertilize with oocytes and decrease the fertility potential of males (p < 0.001).
Conclusion: The use of folic acid and nicotinic acid antioxidants during sperm freezing reduces the harmful effects of free radicals created during the freezing process and helps preserve the fertility potential of Oligospermia men (p < 0.001).
Objective: The aim of this study was to investigate the effect of nicotinic acid and folic acid antioxidants on the sperm motility, survival and deoxyribonucleic acid of oligospermia men during cryopreservation.
Materials and Methods: For this purpose, 25 semen samples of Oligospermia men who referred to the Fertility and Infertility Center of Shahid Beheshti Hospital in Isfahan province were randomly taken into sterile containers and after fluidization, sperm parameters including morphology, motility, and concentration, life and quality of chromatin and their DNA were Measure according to WHO criteria. Then, each sample was divided into 4 parts with freeze-dried medium for freezing: antioxidant-free (control group), 50 nM folic acid, 10 mM nicotinic acid, 50 nM folic acid + 10 mM nicotinic acid. After freezing, the samples were thawed and reexamined for sperm parameters.
Results: Our study showed that the process of freezing sperm by damaging the spermatozoa and cell membrane, resulted in decreased motility and competence of sperm to fertilize with oocytes and decrease the fertility potential of males (p < 0.001).
Conclusion: The use of folic acid and nicotinic acid antioxidants during sperm freezing reduces the harmful effects of free radicals created during the freezing process and helps preserve the fertility potential of Oligospermia men (p < 0.001).
Type of Study: Congress Abstract |
Subject:
Reproductive Endocrinology
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
