International Journal of
Reproductive Biomedicine
Wed, May 14, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 263-263 |
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Koohpeyma F, Dastghaib S, Saki F, Ranjbar Omrani G, Mahmoodi M. P-96 The effect of probiotic Bifidobacterium longum on testis tissue and testosterone hormone in alloxan-diabetic rats. IJRM 2021; 19 (5) :263-263
URL: http://ijrm.ir/article-1-2903-en.html
URL: http://ijrm.ir/article-1-2903-en.html
1- Endocrine and Metabolism Research Center, Shiraz University of Medical Science, Shiraz, Iran.
2- Student Research Committee, Department of Clinical Nutrition, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. ,marzieh.mahmoodi123@gmail.com
2- Student Research Committee, Department of Clinical Nutrition, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. ,
Abstract: (265 Views)
Background: Diabetes is a metabolic endocrine disorder that has a major impact on male reproductive system damages. Probiotics are beneficial microorganisms that could have preventive and therapeutic effects in various metabolic disorders beyond gastrointestinal health.
Objective: The present study carried out to investigate the effectiveness of Probiotic Bifidobacterium longum on the amelioration of some diabetes complications in the testis tissue in diabetic rats.
Materials and Methods: In this study, 30 male rats were randomly divided into three groups including; control, diabetic (induced with Alloxan 120 mg/kg), and diabetic+Bifidobacterium longum. Alloxan was administered intraperitoneally, while the rats in group diabetic+Bifidobacterium longum was fed orally by gavage with 1 mL (1 × 109 CFU/ml/day) of probiotics for 48 days. After dissection, fasting blood glucose, oxidative stress markers, and the amount of tumor necrosis factor-alpha as an inflammatory cytokine were estimated. The rats' testes were quickly removed and put in 10% formalin for further stereological analysis. All data are expressed as mean ± SEM. Statistical analysis was performed using One-way ANOVA followed by Tukey's post hoc tests using SPSS10 (v23) analytic software.
Results: The results showed that malondialdehyde, fasting blood glucose, and tumor necrosis factor-alpha levels decreased, but the level of serum testosterone also anti-oxidant enzymes including superoxide dismutases, and glutathione peroxidase increased significantly in the diabetic group receiving Bifidobacterium longum compared to the diabetic control group (p < 0.05). The evaluation of testis tissue indicated that diabetic rats treated with Bifidobacterium longum significantly increased the number of spermatogonia, spermatocyte, spermatids, a spermatozoid, Leydig cells, and restoration of testis architecture compared to the diabetic group(p < 0.05).
Conclusion: The results of the present study indicated that the Bifidobacterium longum decreased some diabetes complications in the testis tissue. More specifically, our results confirming the protective effects of Bifidobacterium longum through repairing the stereological damages induced by Alloxan. Therefore it might be a good candidate for treatment purposes.
Objective: The present study carried out to investigate the effectiveness of Probiotic Bifidobacterium longum on the amelioration of some diabetes complications in the testis tissue in diabetic rats.
Materials and Methods: In this study, 30 male rats were randomly divided into three groups including; control, diabetic (induced with Alloxan 120 mg/kg), and diabetic+Bifidobacterium longum. Alloxan was administered intraperitoneally, while the rats in group diabetic+Bifidobacterium longum was fed orally by gavage with 1 mL (1 × 109 CFU/ml/day) of probiotics for 48 days. After dissection, fasting blood glucose, oxidative stress markers, and the amount of tumor necrosis factor-alpha as an inflammatory cytokine were estimated. The rats' testes were quickly removed and put in 10% formalin for further stereological analysis. All data are expressed as mean ± SEM. Statistical analysis was performed using One-way ANOVA followed by Tukey's post hoc tests using SPSS10 (v23) analytic software.
Results: The results showed that malondialdehyde, fasting blood glucose, and tumor necrosis factor-alpha levels decreased, but the level of serum testosterone also anti-oxidant enzymes including superoxide dismutases, and glutathione peroxidase increased significantly in the diabetic group receiving Bifidobacterium longum compared to the diabetic control group (p < 0.05). The evaluation of testis tissue indicated that diabetic rats treated with Bifidobacterium longum significantly increased the number of spermatogonia, spermatocyte, spermatids, a spermatozoid, Leydig cells, and restoration of testis architecture compared to the diabetic group(p < 0.05).
Conclusion: The results of the present study indicated that the Bifidobacterium longum decreased some diabetes complications in the testis tissue. More specifically, our results confirming the protective effects of Bifidobacterium longum through repairing the stereological damages induced by Alloxan. Therefore it might be a good candidate for treatment purposes.
Type of Study: Congress Abstract |
Subject:
Fertility & Infertility
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