International Journal of
Reproductive Biomedicine
Tue, May 13, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 181-181 |
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Shafie A, Seifi B, Ashabi G, Kadkhodaee M, Hajiaqai M, Lorian K. P-14 Protective effect of the co-administration of testosterone and sodium hydrosulfide on testicular H2S levels and serum testosterone in experimental model of varicocele. IJRM 2021; 19 (5) :181-181
URL: http://ijrm.ir/article-1-2912-en.html
URL: http://ijrm.ir/article-1-2912-en.html
1- Department of Physiology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran.
2- Department of Physiology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran. ,b-seifi@tums.ac.ir
3- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Department of Physiology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran. ,
3- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abstract: (285 Views)
Background: Androgen secretion is reduced in varicocele. Hydrogen sulfide (H2S), is known as an antioxidant and antiapoptotic molecule.
Objective: This study aimed to assess the effects of co-administration of testosterone and NaHS on sperm count, H2S levels in testicular tissues and serum testosterone in varicocele-induced male rats.
Materials and Methods: Adult male rats were randomly assigned to 5 groups: sham, varicocele, varicocele+testosterone, varicocele+NaHS, varicocele+tetstosterone+NaHS. In the varicocele groups, the left renal vein was partially ligated. In treatment groups, five wk after the induction of varicocele, testosterone (200 μg/kg, subeffective dose) was given subcutaneously for four wk and NaHS (15 μmol/L in drinking water, subeffective dose) were given for four wk. The Left testis tissue samples resected for evaluation H2S levels. The left epididymis tissue also resected for sperm count. blood samples were taken from the inferior vena cava.
Results: Varicocele caused significant reduction in sperm count, testicular H2S levels and serum testosterone compared with the sham group. Administration of testosterone+NaHS significantly increased these parameters compared with varicocele group. But there were no significant changes in these parameters in varicocele+NaHS and varicocele+testosterone group compared with the varicocele group. However, there was a significant enhancement in serum testosterone levels in varicocele+testosterone group compared with the varicocele group but this enhancement was lesser than varicocele+tetstosterone+NaHS group that may due to synergistic effect of NaHS and testosterone.
Conclusion: This study suggested that long term testosterone and NaHS co-administration could improve testicular H2S levels and serum testosterone in varicocele male rats. Therefore, testosterone+NaHS appears to be a useful treatment against varicocele.
Objective: This study aimed to assess the effects of co-administration of testosterone and NaHS on sperm count, H2S levels in testicular tissues and serum testosterone in varicocele-induced male rats.
Materials and Methods: Adult male rats were randomly assigned to 5 groups: sham, varicocele, varicocele+testosterone, varicocele+NaHS, varicocele+tetstosterone+NaHS. In the varicocele groups, the left renal vein was partially ligated. In treatment groups, five wk after the induction of varicocele, testosterone (200 μg/kg, subeffective dose) was given subcutaneously for four wk and NaHS (15 μmol/L in drinking water, subeffective dose) were given for four wk. The Left testis tissue samples resected for evaluation H2S levels. The left epididymis tissue also resected for sperm count. blood samples were taken from the inferior vena cava.
Results: Varicocele caused significant reduction in sperm count, testicular H2S levels and serum testosterone compared with the sham group. Administration of testosterone+NaHS significantly increased these parameters compared with varicocele group. But there were no significant changes in these parameters in varicocele+NaHS and varicocele+testosterone group compared with the varicocele group. However, there was a significant enhancement in serum testosterone levels in varicocele+testosterone group compared with the varicocele group but this enhancement was lesser than varicocele+tetstosterone+NaHS group that may due to synergistic effect of NaHS and testosterone.
Conclusion: This study suggested that long term testosterone and NaHS co-administration could improve testicular H2S levels and serum testosterone in varicocele male rats. Therefore, testosterone+NaHS appears to be a useful treatment against varicocele.
Type of Study: Congress Abstract |
Subject:
Reproductive Pattology
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