Volume 10, Issue 4 (8-2012)                   IJRM 2012, 10(4): 321-328 | Back to browse issues page

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Khanaki K, Motavalizadeh Ardekani A, Ghassemzadeh A, Shahnazi V, Sadeghi M R, Darabi M, et al . Effect of polyunsaturated fatty acids on secretory phospholipase A2 type IIa in ectopic endometrial cells. IJRM 2012; 10 (4) :321-328
URL: http://ijrm.ir/article-1-297-en.html
Effect of polyunsaturated fatty acids on secretory phospholipase A2 type IIa in ectopic endometrial cells
Korosh Khanaki1 , Ali Motavalizadeh Ardekani2 , Alieh Ghassemzadeh3 , Vahideh Shahnazi3 , Mohammad Reza Sadeghi2 , Masoud Darabi1 , Amir Mehdizadeh1 , Abotaleb Saremi4 , Jafar Soleimani-Rad3 , Ali Reza Imani5 , Mohammad Nouri3 , Ali Rahimipour * 6
1- Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran- Iran
3- Women’s Reproductive Health Research Center, Alzahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
4- Sarem Cell Research Center (SCRC), Sarem Women's Hospital, Tehran, Iran
5- Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
6- Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , rahimipour.ali@gmail.com
Abstract:   (2725 Views)
Background: Endometriosis is a common chronic inflammation which leads to infertility and chronic pelvic pain in affected women. Secretory phospholipase A2 type IIa (sPLA2IIa) is an acute phase reactant that is markedly increased in inflammatory disorders.
Objective: To assess the effects of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs) administration in endometrial cells culture on sPLA2IIa level and cell survival comparing homolog ectopic versus eutopic endometrial cells from endometriosis patients.
Materials and Methods: In this experimental study, ectopic and eutopic endometrial tissue samples obtained from 15 endometriosis patients were immediately frozen. After thawing and tissue digestion, mixed stromal and endometrial gland cells were cultured for 8 days in three different culture media; balanced ω-3/ω-6, high ω-3 and high ω-6 PUFAs ratio. Cell survival was measured using 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino) carbonyl-2H- tetrazolium hydroxide (XTT) method and sPLA2IIa level assessed with ELISA technique.
Results: The sPLA2IIa level was significantly higher in the ectopic endometrial cell culture compared to the eutopic group for each of the three matched treatments (balanced, high ω-3 and high ω-6). Also the sPLA2IIa level in the ectopic endometrial cell group was remarkably increased by each of the three PUFAs treatments compared to control condition (p<0.05, p<0.01, p<0.05 respectively). Cell survival in the eutopic group was significantly decreased by high ω-6 culturing compared to control medium (p<0.05).
Conclusion: The increase in sPLA2IIa level in ectopic endometrial cells by fatty acid treatments (especially high ω-3), strengthens the hypothesis that PUFAs stimulate secretion of cytokines leading to increased sPLA2IIa level.
Keywords: Fatty acids, Endometriosis, Secretory phospholipase A2 type IIa, Cell culture
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Type of Study: Original Article |
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