International Journal of
Reproductive Biomedicine
Wed, May 14, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 156-156 |
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Babakhanzadeh E, Khodadadian A, Rostami S, Alipourfard I, Aghaei M, Nazari M, et al . O-46 Testicular expression of TDRD1, TDRD5, TDRD9, and TDRD12 in azoospermia. IJRM 2021; 19 (5) :156-156
URL: http://ijrm.ir/article-1-2979-en.html
URL: http://ijrm.ir/article-1-2979-en.html
Babakhanzadeh E1 
, Khodadadian A2 , Rostami S3 , Alipourfard I4 , Aghaei M2 , Nazari M2 , Hosseinnia M5 , Vahidi Mehrjardi MY1 , Jamshidi Y6 , Ghasemi N *7
, Khodadadian A2 , Rostami S3 , Alipourfard I4 , Aghaei M2 , Nazari M2 , Hosseinnia M5 , Vahidi Mehrjardi MY1 , Jamshidi Y6 , Ghasemi N *7
1- Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Department of Cellular and Molecular Biology, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, Iran.
4- Center of Pharmaceutical Sciences, Faculty of Life Sciences, University of Vienna, Vienna, Austria. School of Pharmacy, Faculty of Sciences, University of Rome Tor Vergata, Rome, Italy.
5- Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
6- Genetics Centre, Molecular and Clinical Sciences Institute, St George’s University of London, London, UK.
7- Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Abortion Research Center, Yazd Reproductive Sicences Institue, Shahid sadoughi University of Medical Sciences, Yazd, Iran. ,nghasemi479@gmail.com
2- Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Department of Cellular and Molecular Biology, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, Iran.
4- Center of Pharmaceutical Sciences, Faculty of Life Sciences, University of Vienna, Vienna, Austria. School of Pharmacy, Faculty of Sciences, University of Rome Tor Vergata, Rome, Italy.
5- Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
6- Genetics Centre, Molecular and Clinical Sciences Institute, St George’s University of London, London, UK.
7- Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Abortion Research Center, Yazd Reproductive Sicences Institue, Shahid sadoughi University of Medical Sciences, Yazd, Iran. ,
Abstract: (308 Views)
Background: Tudor domain-containing proteins (TDRDs) play a critical role in piRNA biogenesis and germ cell development.
Objective: piRNAs, small regulatory RNAs, act by silencing of transposons during germline development and it has recently been shown in animal model studies that defects in TDRD genes can lead to sterility in males.
Materials and Methods: Here we evaluate gene and protein expression levels of four key TDRDs (TDRD1, TDRD5, TDRD9 and TDRD12) in testicular biopsy samples obtained from men with obstructive azoospermia (OA, n = 29), as controls, and various types of non-obstructive azoospermia containing hypospermatogenesis (HP, 28), maturation arrest (MA, n = 30), and Sertoli cell-only syndrome (SCOS, n = 32) as cases. One-way ANOVA test followed by Dunnett’s multiple comparison post-test was used to determine inter-group differences in TDRD gene expression among cases and controls.
Results: The results showed very low expression of TDRD genes in SCOS specimens. Also, the expression of TDRD1 and TDRD9 genes were lower in MA samples compared to OA samples. The expression of TDRD5 significantly reduced in SCOS, MA and HP specimens than the OA specimens. Indeed, TDRD12 exhibited a very low expression in HP specimens in comparison to OA specimens. All these results were confirmed by Western blot technique.
Conclusion: TDRDs could be very important in male infertility, which should be express in certain stages of spermatogenesis.
Objective: piRNAs, small regulatory RNAs, act by silencing of transposons during germline development and it has recently been shown in animal model studies that defects in TDRD genes can lead to sterility in males.
Materials and Methods: Here we evaluate gene and protein expression levels of four key TDRDs (TDRD1, TDRD5, TDRD9 and TDRD12) in testicular biopsy samples obtained from men with obstructive azoospermia (OA, n = 29), as controls, and various types of non-obstructive azoospermia containing hypospermatogenesis (HP, 28), maturation arrest (MA, n = 30), and Sertoli cell-only syndrome (SCOS, n = 32) as cases. One-way ANOVA test followed by Dunnett’s multiple comparison post-test was used to determine inter-group differences in TDRD gene expression among cases and controls.
Results: The results showed very low expression of TDRD genes in SCOS specimens. Also, the expression of TDRD1 and TDRD9 genes were lower in MA samples compared to OA samples. The expression of TDRD5 significantly reduced in SCOS, MA and HP specimens than the OA specimens. Indeed, TDRD12 exhibited a very low expression in HP specimens in comparison to OA specimens. All these results were confirmed by Western blot technique.
Conclusion: TDRDs could be very important in male infertility, which should be express in certain stages of spermatogenesis.
Type of Study: Congress Abstract |
Subject:
Pregnancy Health
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