چهارشنبه 24 اردیبهشت 1404
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دوره 19، شماره 5 - ( 3-1400 )
جلد 19 شماره 5 صفحات 157-157 |
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Jaberi Asl A, Dashti GhR, Sharifi M. O-47 Correlation between long non-coding RNA MALAT1 and HOTAIR expression with sperm parameters and MDA level in infertile men. IJRM 2021; 19 (5) :157-157
URL: http://ijrm.ir/article-1-2980-fa.html
URL: http://ijrm.ir/article-1-2980-fa.html
O-47 Correlation between long non-coding RNA MALAT1 and HOTAIR expression with sperm parameters and MDA level in infertile men. International Journal of Reproductive BioMedicine. 1400; 19 (5) :157-157
چکیده: (279 مشاهده)
Background: Infertility is a common complete disorder, which can be caused by oxidative stress. Accumulating evidence suggest that long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and HOX transcript antisense RNA (HOTAIR) is involved in the regulation of the oxidative stress responses.
Objective: We aimed to investigate the possible expression status of MALAT1 and HOTAIR in the sperm and its correlation between sperm parameters and malondialdehyde (MDA) levels.
Materials and Methods: Specimens were obtained randomly from 25 fertile men and 25 infertile men, aged between 25-55 yr old. Sperm parameters were evaluated by computer-aided sperm analysis. Sperm chromatin quality were assessed by acridine orange staining method. Seminal MDA levels were determined by thiobarbituric acid reaction method. The expression of MALAT1 and HOTAIR was detected by RT-PCR.
Results: A decreased level of MALAT1 and HOTAIR expression was observed to be associated with the infertile patients (p < 0.001). The relative expression level of MALAT1 and HOTAIR were positively correlated with motility and morphology (p < 0.001). Meanwhile we found the expression levels of genes were negatively correlated with sperm chromatin damage and MDA levels (p < 0.001).
Conclusion: The decreased expression of MALAT1 and HOTAIR resulted in high level of MDA, DNA denaturation and abnormal semen parameters. These findings exhibited the important implications of lncRNAs serving as a potential therapeutic indicator to assess male infertility in assisted reproductive procedures.
Objective: We aimed to investigate the possible expression status of MALAT1 and HOTAIR in the sperm and its correlation between sperm parameters and malondialdehyde (MDA) levels.
Materials and Methods: Specimens were obtained randomly from 25 fertile men and 25 infertile men, aged between 25-55 yr old. Sperm parameters were evaluated by computer-aided sperm analysis. Sperm chromatin quality were assessed by acridine orange staining method. Seminal MDA levels were determined by thiobarbituric acid reaction method. The expression of MALAT1 and HOTAIR was detected by RT-PCR.
Results: A decreased level of MALAT1 and HOTAIR expression was observed to be associated with the infertile patients (p < 0.001). The relative expression level of MALAT1 and HOTAIR were positively correlated with motility and morphology (p < 0.001). Meanwhile we found the expression levels of genes were negatively correlated with sperm chromatin damage and MDA levels (p < 0.001).
Conclusion: The decreased expression of MALAT1 and HOTAIR resulted in high level of MDA, DNA denaturation and abnormal semen parameters. These findings exhibited the important implications of lncRNAs serving as a potential therapeutic indicator to assess male infertility in assisted reproductive procedures.
نوع مطالعه: Congress Abstract |
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