International Journal of
Reproductive Biomedicine
Wed, May 14, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 162-162 |
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Aghajanpour S, Hosseini E, Amirchaghmaghi E, Zandieh Z, Amjadi F, Yahyaei A, et al . O-52 Endometrial scratching affects gene expression of NLRP3 in patients with unexplained repeated implantation failure: A randomized control trial. IJRM 2021; 19 (5) :162-162
URL: http://ijrm.ir/article-1-2985-en.html
URL: http://ijrm.ir/article-1-2985-en.html
Aghajanpour S1 
, Hosseini E2 , Amirchaghmaghi E3 , Zandieh Z4 , Amjadi F4 , Yahyaei A4 , Ashrafi M1 , Aflatoonian R *5
, Hosseini E2 , Amirchaghmaghi E3 , Zandieh Z4 , Amjadi F4 , Yahyaei A4 , Ashrafi M1 , Aflatoonian R *5
1- Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
2- Department of Obstetrics and Gynecology, IVF Clinic, Mousavi Hospital, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
3- Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
4- Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
5- Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. ,r.aflatoonian@gmail.com
2- Department of Obstetrics and Gynecology, IVF Clinic, Mousavi Hospital, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
3- Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
4- Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
5- Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. ,
Abstract: (266 Views)
Background: Alternative strategies have been used to augment success rate of implantation in IVF/ICSI cycles in unexplained repeated implantation failure patients. Endometrial scratching is one of these procedures. It seems scratching can affect NLRP3 gene expression which has an important role on receptivity of endometrium. NLRP3 is an intracellular sensor that detects a broad range of harmful sterile or infectious stimuli, resulting in the formation and activation of the NLRP3 inflammasome
Objective: In the present study, we investigated whether gene expression of NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) is affected by endometrial injury during proliferative phase of menstrual cycle before embryo transfer.
Materials and Methods: Twenty women with unexplained repeated implantation failure who failed to conceive during three or more IVF/ICSI cycles and embryo transfer were selected. The patients randomly classified into two study groups (N = 10 in each group). In the intervention group (not in the control group), endometrial scratching was done on day 9-13 in the proliferative phase of the preceding menstrual cycle. Then, endometrial biopsies of the intervention and control groups were performed in the luteal phase (on 19-21 day). The RNA of all samples was extracted and cDNA synthesis was performed. The expression of NLRP3 was quantified by quantitative real-time PCR.
Results:NLRP3 gene expression from all samples was investigated. Relative expression of NLRP3 was lower in the intervention samples compared to the controls.
Conclusion: The inflammasome components are suggested as a novel family of endometrial biomarkers. This result is in consistent with other studies that showed dysregulated inflammasome activation has involved in the disruption of maternal–fetal immune-tolerance and in pregnancy complications.
Objective: In the present study, we investigated whether gene expression of NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) is affected by endometrial injury during proliferative phase of menstrual cycle before embryo transfer.
Materials and Methods: Twenty women with unexplained repeated implantation failure who failed to conceive during three or more IVF/ICSI cycles and embryo transfer were selected. The patients randomly classified into two study groups (N = 10 in each group). In the intervention group (not in the control group), endometrial scratching was done on day 9-13 in the proliferative phase of the preceding menstrual cycle. Then, endometrial biopsies of the intervention and control groups were performed in the luteal phase (on 19-21 day). The RNA of all samples was extracted and cDNA synthesis was performed. The expression of NLRP3 was quantified by quantitative real-time PCR.
Results:NLRP3 gene expression from all samples was investigated. Relative expression of NLRP3 was lower in the intervention samples compared to the controls.
Conclusion: The inflammasome components are suggested as a novel family of endometrial biomarkers. This result is in consistent with other studies that showed dysregulated inflammasome activation has involved in the disruption of maternal–fetal immune-tolerance and in pregnancy complications.
Type of Study: Congress Abstract |
Subject:
Pregnancy Health
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