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Salehnia M. Progesterone Shifts the Pinopodes Expression of Mouse Endometrium to Pre-Implantation Time After Ovarian Hyperstimulation. IJRM 2003; 1 (1) :20-23
URL: http://ijrm.ir/article-1-3-en.html
Progesterone Shifts the Pinopodes Expression of Mouse Endometrium to Pre-Implantation Time After Ovarian Hyperstimulation
Mojdeh Salehnia *
Abstract:   (2933 Views)
Background: The aim of this study was to determine the correlation between ultrastructural studies for pinopodes expression after ovarian hyperstimulation and progesterone injection in mice. Materials and Methods: Adult NMRI mice were superovulated using human menopasual gonadotropic (hMG) and human chorionic gonadotropic (hCG) hormones; after that, daily injection of progesterone (1 mg/mouse) was performed. Animals were sacrificed by cervical dislocation 3.5 and 4.5 days after hCG injection. Tissues of uterine horns were obtained and processed for scanning (SEM) and transmission (TEM) electron microscopy studies. The pseudopregnant control samples were studied same as experimental groups. Results: The SEM and TEM observations showed that in control groups on 3.5 days of pregnancy, there were some pinopodes. All apical cell surfaces expressed these projections on the forth day. In progestrone-injected group, well developed pinopods were expressed 3.5 days after hCG injection and they were transformed to small projections on the fourth day following hCG injection. Also, the life span of pinopods was limited to a short time. At the TEM levels, the pinopods were seen as swelling process on the apical surface, which were more pronounced on day 3.5 of hCG injection in hyperstimulated and progestrone injection. Conclusion: The progestrone may cause premature expression of pinopodes and the implantation failure after ovarian induction may be due to these timing changes.
Keywords: Implantation Window, Pinopodes, Progesterone, Electron Microscopy
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Type of Study: Original Article |
References
1. Ertzeid G, Storeng R. (2001) The impact of ovarion stimulation on implantation and fetal development in mice. Hum Reprod 16: 221-225. [DOI:10.1093/humrep/16.2.221] [PMID]
2. Kolb BA, Najmabadi S, Paulson RJ. (1997) Ultrastructural characteristics of luteal phase endometrium in patients undergoing controlled ovarian hyperstimulation. Feril Steril 67: 625-30. [DOI:10.1016/S0015-0282(97)81356-8]
3. Kramer B, De Wet G. (1994) Exogenous gonadotropin administration affects the glycocalyx of rat endometrial epithelial cells during the period of implantation. J Assist Reprod Gen 11: 504-509. [DOI:10.1007/BF02216030] [PMID]
4. Kramer B, Magan A and De Wet G. (1993). Hyperstimulation affects vascular permeablility at implantation sites in the rat endometrium. J Assist Reprod Gen 10: 163-168. [DOI:10.1007/BF01207741] [PMID]
5. Leesey B.A. (2001a) The role of the endometrium during embryo implantation. Hum Reprod 15: 36-50.
6. Lessey B.A. (2001b) Endometrial receptivity and the window of implantation. Bailieres Best. Pract. Res. Clin Obstet Gynaecol 14: 775-880. [DOI:10.1053/beog.2000.0118] [PMID]
7. Martel D., Monier M.N., Roche D., Psychoyos A. (1991) Hormonal dependence of pinopodes formation at the uterine luminal surface. Hum Reprod 6: 597-603. [DOI:10.1093/oxfordjournals.humrep.a137386] [PMID]
8. Miller BG. (1979) Delayed interactions between progesterone a low doses of 17 β-estradiol in the mouse uterus. Endocrin 104: 26-33. [DOI:10.1210/endo-104-1-26] [PMID]
9. Navot D., Scott R.T., Droesch K., Veeck L.L., Liu H.C., Rosenwaks Z. (1991) The window of embryo transfer and the efficacy of human conception in vitro. Fertil Steril 55: 114-118. [DOI:10.1016/S0015-0282(16)54069-2]
10. Nikas G, Develioglu OH, Toner JP, Jones HWJR. (1999) Endometrial pinopodes indicate a shift in the window of receptivity in IVF cycles. Hum Reprod 14: 787-792. [DOI:10.1093/humrep/14.3.787] [PMID]
11. Nikas G., Drakakis P., Loutradus D., Mara-Shoufari C., Koumantakis E., Michalas S., Psychoyos A. ( 1995) Uterine pinopodes as markers of the nidation window in cycling women receiving exogenous oestradiol and progesterone. Hum Reprod 10: 1208-1213. [DOI:10.1093/oxfordjournals.humrep.a136120] [PMID]
12. Nikas, G, Drakakis P, Loutradus D, Mara-Shoufari C, Koumantakis E, Michala S. (1995) Uterine pinopodes as markers of the " nidation Window" in cycling women receiving exogenous oestradiol and progesterone. Hum Reprod 10: 1208-1213. [DOI:10.1093/oxfordjournals.humrep.a136120] [PMID]
13. Nilsson O. (1958) Ultrastructure of mouse uterine surface epithelium under different estrogenic influences. 2. Early effect of estrogen administrered to spayed animals. J Ultrastruct Res 2: 73-95. [DOI:10.1016/S0022-5320(58)90048-0]
14. Paulson R.J., Saner M.V., Lobo R.A. (1990) Embryo implantation after human in vitro fertilization: importance of endometrial receptivity. Fertil Steril 53: 870-874. [DOI:10.1016/S0015-0282(16)53524-9]
15. Paulson RJ, Sauer MV and Lobo RA. (1997) Potential enhancement of endometrial receptivity in cycles using controlled ovarian hyperstimulation with antiprogestins: a hypthesis. Fertil Steril 67:321-325. [DOI:10.1016/S0015-0282(97)81918-8]
16. Psychoyos A, Nikas G. (1994) Uterine pinopodes as markers of uterine receptivity. Assist Reprod Rev 4: 26-32.
17. Simon C., Cano F., Valbuena D. (1995) Clinical evidence for a detrimental effect on uterine receptivity of high serum estradiol levels in high and normal responder patients. Hum Reprod 10: 2432-2437. [DOI:10.1093/oxfordjournals.humrep.a136313] [PMID]
18. Singh M.M., Chaluhan S.C., Trivedi R. N., Maitra S.C, Kamboj V.P. (1996) Correlation of pinopod development on uterine luminal epithelial surface with hormonal events and endometrial sensitivity in rat. Eur Endocrinol 35: 107-117. [DOI:10.1530/eje.0.1350107] [PMID]
19. Stavreus-Evers A., Nikas G., Sahlin L., Eriksson H., Landgren B.M. (2001) Formation of pinopodes in human endometrium is associated with the concentrations of progesterone and progesterone receptors. Fertil Steril 76: 782-791. [DOI:10.1016/S0015-0282(01)01993-8]
20. Valbuena D, Jasper M, Remon J, Pellicer A, Sima C. (1999) Ovarian stimulation and endometrial receptivity. Hum Reprod 1: 2107-2111. [DOI:10.1093/humrep/14.suppl_2.107] [PMID]
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