International Journal of
Reproductive Biomedicine
Wed, May 14, 2025
[Archive]
Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 288-288 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Akhavansales Zh, Ashrafzadeh HR, Montazeri F, Fakhteh Yavari S, Sharbati A, Tahoori M, et al . P-121 Investigation of association between rs5030772 polymorphism of FasL gene with premature ovarian failure. IJRM 2021; 19 (5) :288-288
URL: http://ijrm.ir/article-1-3032-en.html
URL: http://ijrm.ir/article-1-3032-en.html
Akhavansales Zh1 
, Ashrafzadeh HR2 , Montazeri F2 , Fakhteh Yavari S3 , Sharbati A4 , Tahoori M5 , Bitaraf Sanie M6 , Mirabutalebi SHR2 , Mohamadi M2 , Ghasemi N *7
, Ashrafzadeh HR2 , Montazeri F2 , Fakhteh Yavari S3 , Sharbati A4 , Tahoori M5 , Bitaraf Sanie M6 , Mirabutalebi SHR2 , Mohamadi M2 , Ghasemi N *7
1- Department of Immunology, Faculty of Medicine, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4- Department of Medical Surgical Nursing, School of Nursing and Midwifery, Iran University of Medical Sciences, Tehran, Iran.
5- Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Department of Animal Science Research, Yazd Agriculture and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Yazd, Iran.
7- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,nghasemi479@gmail.com
2- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3- Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4- Department of Medical Surgical Nursing, School of Nursing and Midwifery, Iran University of Medical Sciences, Tehran, Iran.
5- Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
6- Department of Animal Science Research, Yazd Agriculture and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Yazd, Iran.
7- Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ,
Abstract: (211 Views)
Background: Premature ovarian failure (POF), the pathogenesis might be indiscernible for a long time, is related with problems in fertility. In 74-90% of cases, POF is idiopathic but it could be familial (4-33%) or sporadic. The main recognized causes of POF are as follows: Autoimmune ovarian damage, genetic aberrations and environmental factors (i.e. viruses, toxins, etc). Genetic mechanisms of POF are decreased gene dosage as well as non-specific chromosome effect that disrupts meiosis, reducing the pool of primordial follicles along with increasing atresia because of apoptosis or impaired maturation of follicle. Fas ligand (FasL) is a mediator of apoptosis that plays a role in the differentiation of cells and the development of embryo. Polymorphism of FasL gene could be involved in the disease process.
Objective: Considering the importance of apoptosis in homeostasis of normal tissues as well as in disease conditions, we evaluated the impact of a common FasL polymorphism and its relationship with POF.
Materials and Methods: In this case-control study, the polymorphisms of FASLIVS2nt_124A/G were analyzed in Iranian women suffering from POF and healthy controls. Isolation of DNA was done by salting out method and genotype analysis was performed for all the subjects using PCR-RFLP (restriction fragment length-polymerase chain reaction) method.
Results: Statistical analysis revealed no differences in codominant or other models of genotype nor allele frequencies between cases and controls (p > 0.05).
Conclusion: It appears that FasL INV2nt_124A/G rs5030722 SNPs (Single nucleotide polymorphisms) have no difference between POF patients and healthy subjects.
Objective: Considering the importance of apoptosis in homeostasis of normal tissues as well as in disease conditions, we evaluated the impact of a common FasL polymorphism and its relationship with POF.
Materials and Methods: In this case-control study, the polymorphisms of FASLIVS2nt_124A/G were analyzed in Iranian women suffering from POF and healthy controls. Isolation of DNA was done by salting out method and genotype analysis was performed for all the subjects using PCR-RFLP (restriction fragment length-polymerase chain reaction) method.
Results: Statistical analysis revealed no differences in codominant or other models of genotype nor allele frequencies between cases and controls (p > 0.05).
Conclusion: It appears that FasL INV2nt_124A/G rs5030722 SNPs (Single nucleotide polymorphisms) have no difference between POF patients and healthy subjects.
Type of Study: Congress Abstract |
Subject:
Perinatology
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
