Volume 10, Issue 5 (10-2012)                   IJRM 2012, 10(5): 453-458 | Back to browse issues page

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Mahmoudi R, Rajaei F, Ragardi Kashani I, Abbasi M, Amidi F, Sobhani A et al . The rate of blastocysts production following vitrification with step-wise equilibration of immature mouse oocytes. IJRM 2012; 10 (5) :453-458
URL: http://ijrm.ir/article-1-310-en.html
The rate of blastocysts production following vitrification with step-wise equilibration of immature mouse oocytes
Reza Mahmoudi1 , Farzad Rajaei2 , Iraj Ragardi Kashani3 , Mehdi Abbasi3 , Fardin Amidi3 , Aligholi Sobhani3 , Iraj Amiri * 4
1- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
2- Department of Anatomy and Infertility Centre, Qazvin University of Medical Sciences, Qazvin, Iran
3- Department of Anatomy and Embryology, Tehran University of Medical Sciences, Tehran, Iran
4- Research Center for Endometr and Endometriosis, Hamedan University of Medical Sciences, Hamedan, Iran , amiri44@yahoo.com
Abstract:   (3294 Views)
Background: Cryopreservation and in vitro maturation (IVM) of oocyte is becoming an important technique in infertility treatment and fertility preservation. Also it has been proposed to establish a genetic resource bank for endangered or commercially important animal species.
Objective: The aim of this study was to evaluate viability, maturation and fertilization rate of mouse immature oocytes after single and stepwise vitrification procedure.
Materials and Methods: Oocytes were obtained from 4 weeks old female mice 48h after intraperitoneal injection of 7.5 IU pregnant mare serum gonadotropin (PMSG). Collected oocytes before vitrification were exposed to cryoprotectant, which was composed of 30% (v/v) ethylene glycol, 18% (w/v) Ficoll-70, and 0.3 M sucrose, either by single step or in a step-wise way. After vitrification and storage in liquid nitrogen, the oocytes were warmed and washed two times in medium TCM199 and then subjected to IVM, fertilization and subsequent development to blastocysts.
Results: The oocytes survival rates after vitrifying-warming (88.96%), maturation rate (73.23%), the capacity of fertilization (57.80%) and embryonic development to blastocyst (16.41%) in the step-wise exposure were significantly higher (p<0.001) compared with corresponding rate in the single step procedure.
Conclusion: The results suggest that vitrification with step-wise procedure has positive effects on maturation and developmental capacity of mice germinal vesicle oocytes in compare with single step vitrification procedure.
Keywords: Vitrification, Germinal Vesicle stage Oocyte, IVM, Mouse.
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Type of Study: Original Article |
References
1. Atabay EC, Takahashi Y, Katagiri S, Nagano M, Koga A, Kanai Y. Vitrification of bovine oocytes and its application to intergeneric somatic cell nucleus transfer. Theriogenology 2004; 61: 15-23. [DOI:10.1016/S0093-691X(03)00179-1]
2. Amorim CA, Goncàlves PB, Figueiredo JR. Cryopreservation of oocytes from pre-antral follicles. Hum Rep Update 2003; 9: 119-129. [DOI:10.1093/humupd/dmg014]
3. Paynter SJ. Current status of the cryopreservation of human unfertilized oocytes. Hum Reprod Update 2000; 6: 449-456. [DOI:10.1093/humupd/6.5.449]
4. Mahmoudi R, Amiri I, Pasbakhsh P, Ragardi Kashani I, Abbasi M, Farid Aboulhasani F, et al. The effects of vitrification on spindle apparatus of invitro matured germinal vesicle in mice. Iran J Reprod Med 2008; 6: 209-215.
5. Trounson AO, Wood C, Kausche A. IVM and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril 1994; 62: 353-362. [DOI:10.1016/S0015-0282(16)56891-5]
6. Wu J, Zhang L, Wang X. IVM, fertilization and embryo development after ultra-rapid freezing of immature human oocytes. Reproduction 2001; 121: 389-393. [DOI:10.1530/rep.0.1210389]
7. Cao Y, Xing Q, Zhang ZG, Wei ZL, Zhou P, Cong L. Cryopreservation of immature and in-vitro matured human oocytes by vitrification. Reprod Biomed Online 2009; 19: 369-373. [DOI:10.1016/S1472-6483(10)60170-8]
8. Son WY, Park SE, Lee KA, Lee WS, Ko JJ, Yoon TK, et al. Effects of 1,2-propanediol and freezing-thawing on the in vitro developmental capacity of human immature oocytes. Fertil Steril 1996; 66: 995-999. [DOI:10.1016/S0015-0282(16)58696-8]
9. Schroeder AC, Eppig JJ. The developmental capacity of mouse oocytes that matured spontaneously in vitro is normal. Dev Biol 1984; 102: 493-497. [DOI:10.1016/0012-1606(84)90215-X]
10. Mahmoudi R, Sobhani A, Pasbakhsh P, Abolhasani F, Amiri I, Salehnia M, et al. The Effects of cumulus cells on IVM of mouse germinal vesicle stage oocytes. Iran J Reprod Med 2005; 3: 74-78.
11. Mahmodi R, Abbasi M, Amiri I, Ragardi Kashani I, Pasbakhsh P, Saadipour Kh, et al. Cumulus cell role on mouse germinal vesicle oocyte maturation, fertilization, and subsequent embryo development to blastocyst stage in vitro. Cell Journal 2009; 11: 299-302.
12. Nagai T. The improvement of IVM systems for bovine and porcine oocytes. Theriogenology 2001; 55: 1291-1301. [DOI:10.1016/S0093-691X(01)00483-6]
13. Khosravi-Farsani S, Sobhani A, Amidi F, Mahmoudi R. Mouse oocyte vitrification: the effects of two methods on maturing germinal vesicle breakdown oocytes. J Assist Reprod Genet 2010; 27: 233-238. [DOI:10.1007/s10815-010-9411-x]
14. Anderiesz C, Trounson AO. The effect of testosterone on the maturation and developmental capacity of murine oocytes in vitro. Hum Reprod 1995; 10: 2377-2381. [DOI:10.1093/oxfordjournals.humrep.a136302]
15. Chang M. The maturation of rabbit oocytes in culture and their maturation, activation, fertilization, and subsequent development in the fallopian tubes. J Exp Zool 1955; 128: 378-405. [DOI:10.1002/jez.1401280207]
16. Hashimoto S, Saeki K, Nagao Y, Minami N, Yamada M, Utsumi K. Effects of cumulus cell density during IVM of the developmental competence of bovine oocytes. Theriogenology 1998; 49: 1451-1463. [DOI:10.1016/S0093-691X(98)00091-0]
17. Goud PT, Goud AP, Qian C, Laverge H, Van der Elst J, De Sutter P , et al. In-vitro maturation of human germinal vesicle stage oocytes: role of cumulus cells and epiderminal growth factor in the culture medium. Hum Reprod 1998; 13: 1638-1644. [DOI:10.1093/humrep/13.6.1638]
18. Anderiesz C, Ferraretti A, Magli C, Fiorentino A, Fortini D, Gianaroli L, et al. Effect of recombinant human gonadotropins on human, bovine and murine oocyte meiosis, fertilization and embryonic development in vitro. Hum Reprod 2000; 15: 1140-1148. [DOI:10.1093/humrep/15.5.1140]
19. Zhiguo Z, Yu Liu, Qiong X, Ping Z, Yunxia C. Cryopreservation of human failed-matured oocytes followed by in vitro maturation: vitrification is superior to the slow freezing method. Reprod Biol Endocrinol 2011; 9: 156-163. [DOI:10.1186/1477-7827-9-156]
20. Kassai M, Komi JH, Takakamo A, Tsudera H, Sakurai T. Asimple method for mouse embryo cryopreservation in low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fertil 1999; 89: 91-97. [DOI:10.1530/jrf.0.0890091]
21. Abe Y, Hara K, Matsumoto H, Kobayashi J, Sasada H, Ekwall H, et al. Feasibility of a nylon-mesh holder for vitrification of bovine germinal vesicle oocytes in subsequent production of viable blastocysts. Biol Reprod 2005; 72: 1416-1420. [DOI:10.1095/biolreprod.104.037051]
22. Cooper A, Paynter SJ, Fuller BJ, Shaw RW. Differential effects of cryopreservation on nuclear or cytoplasmic maturation in vitro in immature mouse oocytes from stimulated ovaries. Hum Reprod 1998; 13: 971-978. [DOI:10.1093/humrep/13.4.971]
23. Wongsrikeao P, Kaneshige Y, Ooki R, Taniguchi M, Agung B, Nii M, et al. Effect of the removal of cumulus cell on the nuclear maturation, fertilization and development of porcine oocytes. Reprod Dom Amin 2005; 40: 166-170. [DOI:10.1111/j.1439-0531.2005.00576.x]
24. Hochi S, Ito K, Hirabayashi M, Ueda M, Kimura K, Hanada A. Effect of nuclear stages during IVM on the survival of vitirified-warmed bovine oocytes. Theriogenology 1998; 49: 787-796. [DOI:10.1016/S0093-691X(98)00028-4]
25. Saragusty J, Arav A. Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Reproduction 2011; 141: 1-19. [DOI:10.1530/REP-10-0236]
26. Hurt AE, Landim F, Seidel GE, Squires EL. Vitrification of immature and mature equine and bovine oocytes in an ethylene glycol, ficoll and sucrose solution using open-pulled straws. Theriogenology 2000; 54: 119-128. [DOI:10.1016/S0093-691X(00)00330-7]
27. Cetin Y, Bastan A. Cryopreservation of immature bovine oocytes by vitrification in straws. Anim Reprod Sci 2006; 92: 29-36. [DOI:10.1016/j.anireprosci.2005.05.016]
28. Aono N, Naganuma T, Abe Y, Hara K, Sasada H, Sato E, et al. Successful production of blastocysts following ultrarapid vitrification with step-wise equilibriation of germinal vesicle-stage mouse oocytes. J Reprod Dev 2003; 49: 501-506. [DOI:10.1262/jrd.49.501]
29. Aono N, Abe Y, Hara K, Sasada H, Sato E, Yoshida H. Production of live offspring from mouse germinal vesicle-stage oocytes vitrified by a modified stepwise method, SWEID. Fertil Steril 2005; 84 (Suppl.): 1078-1082. [DOI:10.1016/j.fertnstert.2005.03.077]
30. Kuwayama M. Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology 2007; 67: 73-80. [DOI:10.1016/j.theriogenology.2006.09.014]
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