Volume 10, Issue 6 (4-2012)                   IJRM 2012, 10(6): 571-580 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Soleimani Mehranjani M, Taefi R. The protective role of vitamin E on the testicular tissue in rats exposed to sodium arsenite during the prenatal stage till sex maturity: A stereological analysis. IJRM 2012; 10 (6) :571-580
URL: http://ijrm.ir/article-1-320-en.html
The protective role of vitamin E on the testicular tissue in rats exposed to sodium arsenite during the prenatal stage till sex maturity: A stereological analysis
Malek Soleimani Mehranjani * 1, Rezvan Taefi2
1- Department of Biology, Faculty of Sciences, Arak University, Arak, Iran , M-soleimani@araku.ac.ir
2- Department of Biology, Faculty of Sciences, Arak University, Arak, Iran
Abstract:   (2815 Views)
Background: Vitamin E is an effective antioxidant, protecting cells against oxidative stress.
Objective: In this investigation the protective effect of vitamin E on the testis during development and spermatogenesis in rats exposed to sodium arsenite was evaluated.
Materials and Methods: Pregnant Wistar rats were divided into 4 groups (n=8) control, sodium arsenite (8 mg/kg/day), sodium arsenite+vitamin E (100 mg/kg/day) and vitamin E. Treatment was carried out from day seven of pregnancy till 90 days. Finally the right testis was stereologically studied. The obtained data was analyzed using one way ANOVA and Tukey's test and the means difference was considered significant at p<0.05.
Results: The weight and volume of testis, volume of seminiferous tubules and its diameter, volume of interstitial tissue, height of germinal epithelium and the total number of types A and B spermatogonia, spermatocyte, spermatid and sertoli cells reduced significantly in sodium arsenite group compared to the control. Co-administration of vitamin E and sodium arsenite compensated the adverse effects of sodium arsenite on the above parameters.
Conclusion: We concluded co-treatment of rats with sodium arsenite and vitamin E could prevent the adverse effects of sodium arsenite exposure on the testicular tissue during the prenatal stage till sex maturity.
Keywords: Sodium arsenite, Vitamin E, Stereology, Testis, Rat
Full-Text [PDF 586 kb]   (701 Downloads) |   |   Full-Text (HTML)  (327 Views)  
Type of Study: Original Article |
References
1. Pant N, Murthy RC, Srivastava SP. Male reproductive toxicity of sodium arsenite in mice. Hum Exp Toxicol 2004; 23: 399-403. [DOI:10.1191/0960327104ht467oa]
2. Liu SX, Athar M, Lippai I, Waldren C, Hei TK. Induction of oxyradicals by arsenic: implication for mechanism of genotoxicity. Proc Natl Acad Sci USA 2001; 98: 1643-1648. [DOI:10.1073/pnas.98.4.1643]
3. Jana K, Jana S, Samanta PK. Effects of chronic exposure to sodium arsenite on hypothalamo-pituitary-testicular activities in adult rats: possible an estrogenic mode of action. Reprod Biol Endocrinol 2006; 4: 9. [DOI:10.1186/1477-7827-4-9]
4. Tseng CH, Tseng CP, Chiou HY, Hsueh YM, Chong CK, Chen CJ. Epidemiologic evidence of diabetogenic effect of arsenic. Toxicol Lett 2002; 133: 69-76. [DOI:10.1016/S0378-4274(02)00085-1]
5. Goebel HH, Schmidt PF, Bohl J, Tettenborn B, Kramer G, Gutmann L. Polyneuropathy due to acute arsenic intoxication: biopsy studies. J Neuropathol Exp Neurol 1990; 49: 137-149. [DOI:10.1097/00005072-199003000-00006]
6. Neiger RD, Osweiler GD. Effect of subacute low level dietary sodium arsenite on dogs. Fundam Appl Toxicol 1989; 13: 439-451. [DOI:10.1016/0272-0590(89)90281-9]
7. Pant N, Shankar R, Srivastava SP. In utero and lactational exposure of carbofuran to rats: effect on testes and sperm. Hum Exp Toxicol 1997; 16: 267-272. [DOI:10.1177/096032719701600506]
8. Biswas R, Poddar S, Mukherjee A. Investigation on the genotoxic effects of long-term administration of sodium arsenite in bone marrow and testicular cells in vivo using the comet assay. J Environ Pathol Toxicol Oncol 2007; 26: 29-37. [DOI:10.1615/JEnvironPatholToxicolOncol.v26.i1.40]
9. Sarkar M, Chaudhuri GR, Chattopadhyay A, Biswas NM. Effect of sodium arsenite on spermatogenesis, plasma gonadotrophins and testosterone in rats. Asian J Androl 2003; 5: 27-31.
10. Dhar P, Somesh M, Kaushal P, Seghal R, Mehra R. Effects of arsenic exposure durnig early posnatal period on Leydig cells of rats testis. Toxicol Environmen Chem 2010; 92: 1157-1168. [DOI:10.1080/02772240903306334]
11. Papadopooulus V. Environmental factors that disrupt Leydig cell streoidogenesis. Springer; 2007.
12. Hood RD, Vedel GC, Zaworotko MJ, Tatum FM, Meeks RG. Uptake, distribution, and metabolism of trivalent arsenic in the pregnant mouse. J Toxicol Environ Health 1988; 25: 423-434. [DOI:10.1080/15287398809531221]
13. Holson JF, Stump DG, Ulrich CE, Farr CH. Absence of prenatal developmental toxicity from inhaled arsenic trioxide in rats. Toxicol Sci 1999; 51: 87-97. [DOI:10.1093/toxsci/51.1.87]
14. Stump DG, Holson JF, Fleeman TL, Nemec MD, Farr CH. Comparative effects of single intraperitoneal or oral doses of sodium arsenate or arsenic trioxide during in utero development. Teratology 1999; 60: 283-291. https://doi.org/10.1002/(SICI)1096-9926(199911)60:5<283::AID-TERA9>3.0.CO;2-7 [DOI:10.1002/(SICI)1096-9926(199911)60:53.0.CO;2-7]
15. Chen A, Cao EH, Zhang TC, Qin JF. Arsenite-induced reactive oxygen species and the repression of alpha-tocopherol in the MGC-803 cells. Eur J Pharmacol 2002; 448: 11-18. [DOI:10.1016/S0014-2999(02)01901-5]
16. El-Demerdash FM. Antioxidant effect of vitamin E and selenium on lipid peroxidation, enzyme activities and biochemical parameters in rats exposed to aluminium. J Trace Elem Med Biol 2004; 18: 113-121. [DOI:10.1016/j.jtemb.2004.04.001]
17. Traber MG, Atkinson J. Vitamin E, antioxidant and nothing more. Free Radic Biol Med 2007; 43: 4-15. [DOI:10.1016/j.freeradbiomed.2007.03.024]
18. Chitra KC, Mathur PP. Vitamin E prevents nonylphenol-induced oxidative stress in testis of rats. Ind J Exp Biol 2004; 42: 220-223.
19. Sahinturk V, Guclu C, Baycu C. Protective effects of vitamin E on ethane dimethane sulfonate-induced testicular toxicity in rats. Asian J Androl 2007; 9: 117-124. [DOI:10.1111/j.1745-7262.2007.00229.x]
20. Chinoy N, Sharma A, Patel T, Memon R, Jhala D. Recovery from fluoride and aluminum induced free radical liver toxicity in mice. Fluoride 2004; 37: 257-263.
21. Zhou DX, Qiu SD, Zhang J, Tian H, Wang HX. The protective effect of vitamin E against oxidative damage caused by formaldehyde in the testes of adult rats. Asian J Androl 2006; 8: 584-588. [DOI:10.1111/j.1745-7262.2006.00198.x]
22. Chen H, Liu J, Luo L, Baig MU, Kim JM, Zirkin BR. Vitamin E, aging and Leydig cell steroidogenesis. Exp Gerontol 2005; 40: 728-736. [DOI:10.1016/j.exger.2005.06.004]
23. Butkevich IP, Vershinina EA. [Nociceptive sensitivity to longterm exposure to an irritant in formaline test of males and females of rat in postnatal development]. Zh Evol Biokhim Fiziol 2005; 41: 76-81.
24. de Jager C, Bornman MS, Oosthuizen JM. The effect of p-nonylphenol on the fertility potential of male rats after gestational, lactational and direct exposure. Andrologia 1999; 31: 107-113. [DOI:10.1046/j.1439-0272.1999.00246.x]
25. de Jager C, Bornman MS, van der Horst G. The effect of p-nonylphenol, an environmental toxicant with oestrogenic properties, on fertility potential in adult male rats. Andrologia 1999; 31: 99-106. [DOI:10.1046/j.1439-0272.1999.00245.x]
26. Acharya UR, Mishra M, Patro J, Panda MK. Effect of vitamins C and E on spermatogenesis in mice exposed to cadmium. Reprod Toxicol 2008; 25: 84-88. [DOI:10.1016/j.reprotox.2007.10.004]
27. Mandarim-de-Lacerda CA. Stereological tools in biomedical research. An Acad Bras Cienc 2003; 75: 469-486. [DOI:10.1590/S0001-37652003000400006]
28. Latendresse JR, Warbrittion AR, Jonassen H, Creasy DM. Fixation of testes and eyes using a modified Davidson's fluid: comparison with Bouin's fluid and conventional Davidson's fluid. Toxicol Pathol 2002; 30: 524-533. [DOI:10.1080/01926230290105721]
29. Zhang H, Zeng X, Cheng W, Wu D. [Adverse effects of nonylphenol on the reproductive function of adult male SD rats]. Sichuan Da Xue Xue Bao Yi Xue Ban 2003; 34: 295-297.
30. Zhang H, Long DM, Zhan L, Wu DS. [Effects of nonylphenol on testis tissue development and apoptosis of F1 generation male SD rats in weaning]. Sichuan Da Xue Xue Bao Yi Xue Ban 2006; 37: 421-423.
31. Latchoumycandane C, Mathur PP. Effects of vitamin E on reactive oxygen species-mediated 2,3,7,8-tetrachlorodi-benzo-p-dioxin toxicity in rat testis. J Appl Toxicol 2002; 22: 345-351. [DOI:10.1002/jat.866]
32. Ahmad I, Hussain T, Akthar K. Arsenic induced microscopic changes in rat testis. Prof Med J 2008; 15: 287-291.
33. Manna P, Sinha M, Sil PC. Protection of arsenic-induced testicular oxidative stress by arjunolic acid. Redox Rep 2008; 13: 67-77. [DOI:10.1179/135100008X259169]
34. Sanghamitra S, Hazra J, Upadhyay SN, Singh RK, Amal RC. Arsenic induced toxicity on testicular tissue of mice. Ind J Physiol Pharmacol 2008; 52: 84-90.
35. Chang SI, Jin B, Youn P, Park C, Park JD, Ryu DY. Arsenic-induced toxicity and the protective role of ascorbic acid in mouse testis. Toxicol Appl Pharmacol 2007; 218: 196-203. [DOI:10.1016/j.taap.2006.11.009]
36. Waalkes MP, Ward JM, Liu J, Diwan BA. Transplacental carcinogenicity of inorganic arsenic in the drinking water: induction of hepatic, ovarian, pulmonary, and adrenal tumors in mice. Toxicol Appl Pharmacol 2003; 186: 7-17. [DOI:10.1016/S0041-008X(02)00022-4]
37. Dalgaard M, Pilegaard K, Ladefoged O. In utero exposure to diethylstilboestrol or 4-n-nonylphenol in rats: number of sertoli cells, diameter and length of seminiferous tubules estimated by stereological methods. Pharmacol Toxicol 2002; 90: 59-65. [DOI:10.1034/j.1600-0773.2002.900202.x]
38. Mittal M, Flora SJ. Vitamin E supplementation protects oxidative stress during arsenic and fluoride antagonism in male mice. Drug Chem Toxicol 2007; 30: 263-281. [DOI:10.1080/01480540701380075]
39. Pal S, Chatterjee AK. Possible beneficial effects of melatonin supplementation on arsenic-induced oxidative stress in Wistar rats. Drug Chem Toxicol 2006; 29: 423-433. [DOI:10.1080/01480540600837993]
40. El-Demerdash FM, Yousef MI, Radwan FM. Ameliorating effect of curcumin on sodium arsenite-induced oxidative damage and lipid peroxidation in different rat organs. Food Chem Toxicol 2009; 47: 249-254. [DOI:10.1016/j.fct.2008.11.013]
41. Suzuki S, Arnold LL, Ohnishi T, Cohen SM. Effects of inorganic arsenic on the rat and mouse urinary bladder. Toxicol Sci 2008; 106: 350-363. [DOI:10.1093/toxsci/kfn184]
42. Balakumar B, Ramanathan K, Kumaresan S, Swesh R. DNA damage by sodium arsenite in experimental rats: ameliorative effects of antioxidant vitamins C and E. Ind J Sci Tech 2010; 3: 322-327.
43. Liu SX, Davidson MM, Tang X, Walker WF, Athar M, Ivanov V, et al. Mitochondrial damage mediates 1. Pant N, Murthy RC, Srivastava SP. Male reproductive toxicity of sodium arsenite in mice. Hum Exp Toxicol 2004; 23: 399-403. [DOI:10.1191/0960327104ht467oa]
44. Del Razo LM, Styblo M, Cullen WR, Thomas DJ. Determination of trivalent methylated arsenicals in biological matrices. Toxicol Appl Pharmacol 2001; 174: 282-293. [DOI:10.1006/taap.2001.9226]
45. Gavazza M, Catala A. The effect of alpha-tocopherol on the lipid peroxidation of mitochondria and microsomes obtained from rat liver and testis. Mol Cell Biochem 2001; 225: 121-128. [DOI:10.1023/A:1012274206337]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb