International Journal of
Reproductive Biomedicine
Mon, Dec 30, 2024
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Volume 22, Issue 7 (July 2024)
IJRM 2024, 22(7): 527-538 |
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Chegini R, Sadeghi M, Shirian S, Sabbaghziarani F, Aali E, Soleimani P, et al . Effects of combination of melatonin and L-carnitine on in vitro maturation in mouse oocytes: An experimental study. IJRM 2024; 22 (7) :527-538
URL: http://ijrm.ir/article-1-3240-en.html
URL: http://ijrm.ir/article-1-3240-en.html
Raziye Chegini1 
, Morteza Sadeghi2 , Sadegh Shirian3 , Fatemeh Sabbaghziarani4 , Ehsan Aali5 , Pouriya Soleimani6 , Mohammad Reza Ashtari Majelan5 , Fariba Zafari * 7, Shahram Darabi5
, Morteza Sadeghi2 , Sadegh Shirian3 , Fatemeh Sabbaghziarani4 , Ehsan Aali5 , Pouriya Soleimani6 , Mohammad Reza Ashtari Majelan5 , Fariba Zafari * 7, Shahram Darabi5
1- Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
2- Human Genetic Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
3- Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran. & Shiraz Molecular Pathology Research Center, Dr. Daneshbod Path Lab, Shiraz, Iran.
4- Cellular and Molecular Research Center, Research Institute for Prevention of Non-communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran. & Shiraz Molecular Pathology Research Center, Dr. Daneshbod Path Lab, Shiraz, Iran.
5- Cellular and Molecular Research Center, Research Institute for Prevention of Non-communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran.
6- Department of Nursing, Faculty of Nursing and Midwifery, Qazvin University of Medical Sciences, Qazvin, Iran.
7- Cellular and Molecular Research Center, Research Institute for Prevention of Non-communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran. ,f.zafari@qums.ac.ir
2- Human Genetic Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
3- Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran. & Shiraz Molecular Pathology Research Center, Dr. Daneshbod Path Lab, Shiraz, Iran.
4- Cellular and Molecular Research Center, Research Institute for Prevention of Non-communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran. & Shiraz Molecular Pathology Research Center, Dr. Daneshbod Path Lab, Shiraz, Iran.
5- Cellular and Molecular Research Center, Research Institute for Prevention of Non-communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran.
6- Department of Nursing, Faculty of Nursing and Midwifery, Qazvin University of Medical Sciences, Qazvin, Iran.
7- Cellular and Molecular Research Center, Research Institute for Prevention of Non-communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran. ,
Abstract: (497 Views)
Background: Melatonin and L-carnitine are free radical scavengers with antiapoptotic and antioxidant properties that improve oocyte development.
Objective: This study aimed to find the possible effect of combining 2 antioxidant agents of melatonin and L-carnitine on oocyte morphology, maturation, apoptosis, and expression of bone morphogenetic protein 15 (BMP-15) and growth differentiation factor 9 (GDF-9) genes in a mice model.
Materials and Methods: To overstimulation, 60 female NMRI mice were injected intraperitoneally using mare serum gonadotropin. On day 2 post-injection, 70 cumulus-oocyte complexes were collected from each mouse. The collected oocytes randomly were then divided into 4 groups including, the control, melatonin, L-carnitine, melatonin + L-carnitine groups. The morphology and maturation rate of the oocytes was evaluated using a light microscope. Apoptosis was identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and the expression of BMP-15 and growth and differentiation factor GDF-9 genes was also evaluated by real-time polymerase chain reaction.
Results: Oocyte diameter significantly was increased in combination treatment of L-carnitine and melatonin compared to other groups (p < 0.05). L-carnitine group showed the highest mean percentage of oocyte cytoplasmic pattern. Results of the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling indicated that the lowest apoptosis rate belonged to the melatonin + L-carnitine group. Moreover, the combination groups showed the highest number of oocytes and maturation rate. The BMP-15 and GDF-9 genes were significantly upregulated in all treatment groups compared to the control group.
Conclusion: Our results suggested a combination of melatonin + L-carnitine administration as a more effective choice for in vitro promotion of oocyte maturation.
Objective: This study aimed to find the possible effect of combining 2 antioxidant agents of melatonin and L-carnitine on oocyte morphology, maturation, apoptosis, and expression of bone morphogenetic protein 15 (BMP-15) and growth differentiation factor 9 (GDF-9) genes in a mice model.
Materials and Methods: To overstimulation, 60 female NMRI mice were injected intraperitoneally using mare serum gonadotropin. On day 2 post-injection, 70 cumulus-oocyte complexes were collected from each mouse. The collected oocytes randomly were then divided into 4 groups including, the control, melatonin, L-carnitine, melatonin + L-carnitine groups. The morphology and maturation rate of the oocytes was evaluated using a light microscope. Apoptosis was identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and the expression of BMP-15 and growth and differentiation factor GDF-9 genes was also evaluated by real-time polymerase chain reaction.
Results: Oocyte diameter significantly was increased in combination treatment of L-carnitine and melatonin compared to other groups (p < 0.05). L-carnitine group showed the highest mean percentage of oocyte cytoplasmic pattern. Results of the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling indicated that the lowest apoptosis rate belonged to the melatonin + L-carnitine group. Moreover, the combination groups showed the highest number of oocytes and maturation rate. The BMP-15 and GDF-9 genes were significantly upregulated in all treatment groups compared to the control group.
Conclusion: Our results suggested a combination of melatonin + L-carnitine administration as a more effective choice for in vitro promotion of oocyte maturation.
Type of Study: Original Article |
Subject:
Assisted Reproductive Technologies
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