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Volume 22, Issue 7 (July 2024)
IJRM 2024, 22(7): 515-526 |
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Akbarzadeh M, Ataee R, Nemati F, Depouri A A, Shaki F. Melatonin's protective effect against placental transfer of Methadone in mice: An experimental study. IJRM 2024; 22 (7) :515-526
URL: http://ijrm.ir/article-1-3257-en.html
URL: http://ijrm.ir/article-1-3257-en.html
Melatonin's protective effect against placental transfer of Methadone in mice: An experimental study
1- Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Qaemshahr Branch, Qaemsharh, Iran.
2- Medicinal Plants Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
3- Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Qaemshahr Branch, Qaemsharh, Iran. ,farkhondehnemati@gmail.com
4- Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
2- Medicinal Plants Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
3- Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Qaemshahr Branch, Qaemsharh, Iran. ,
4- Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Abstract: (452 Views)
Background: Methadone is a substance widely used in the substitution treatment of opiate addiction in pregnancy. The placental transfer of methadone influences oxidative stress processes. Melatonin is a hormone with antioxidant activity.
Objective: This study aimed to evaluate the protective effects of melatonin on oxidative stress induced by the transfer of transplacental methadone in mice.
Materials and Methods: In this experimental study, 36 female mice (2 months old, 20 ± 2 gr) were divided into 6 groups (n = 6/each) of control, methadone (0.3 mg/kg intraperitoneal, single dose) and melatonin (2, 4, and 6 mg/kg/day gavage) were administered 30 min before methadone, and one group received melatonin alone (0.6 mg/kg with single injection). Administration for 10 consecutive days of the pregnancy period was done. After baby mice were born, all neonatal mice were killed by beheading or sacrificing after anesthesia. The liver tissues were extracted. The samples were then sent for studying oxidative stress markers such as lipid peroxidation, glutathione, and protein carbonyl contents. Also, we have used the immunohistochemistry method for apoptotic markers such as: BAX, Bcl2, and Caspase3 for assaying apoptosis.
Results: This study has shown that methadone caused a significant decrease in glutathione concentration (p = 0.035). Also, we observed a significant increase in lipid peroxidation and protein carbonyl contents (p = 0.015, 0.025 respectively). However, melatonin treatment significantly inhibited oxidative stress markers (p = 0.025). Also, apoptosis assay has shown that melatonin could decrease BAX and Caspase 9 as apoptotic and increase Bcl2 as an antiapoptotic proteins (p = 0.015).
Conclusion: Our findings have shown that melatonin has a protective effect against oxidative stress and apoptosis induced by the placental transfer of methadone via its antioxidant effects.
Objective: This study aimed to evaluate the protective effects of melatonin on oxidative stress induced by the transfer of transplacental methadone in mice.
Materials and Methods: In this experimental study, 36 female mice (2 months old, 20 ± 2 gr) were divided into 6 groups (n = 6/each) of control, methadone (0.3 mg/kg intraperitoneal, single dose) and melatonin (2, 4, and 6 mg/kg/day gavage) were administered 30 min before methadone, and one group received melatonin alone (0.6 mg/kg with single injection). Administration for 10 consecutive days of the pregnancy period was done. After baby mice were born, all neonatal mice were killed by beheading or sacrificing after anesthesia. The liver tissues were extracted. The samples were then sent for studying oxidative stress markers such as lipid peroxidation, glutathione, and protein carbonyl contents. Also, we have used the immunohistochemistry method for apoptotic markers such as: BAX, Bcl2, and Caspase3 for assaying apoptosis.
Results: This study has shown that methadone caused a significant decrease in glutathione concentration (p = 0.035). Also, we observed a significant increase in lipid peroxidation and protein carbonyl contents (p = 0.015, 0.025 respectively). However, melatonin treatment significantly inhibited oxidative stress markers (p = 0.025). Also, apoptosis assay has shown that melatonin could decrease BAX and Caspase 9 as apoptotic and increase Bcl2 as an antiapoptotic proteins (p = 0.015).
Conclusion: Our findings have shown that melatonin has a protective effect against oxidative stress and apoptosis induced by the placental transfer of methadone via its antioxidant effects.
Type of Study: Original Article |
Subject:
Reproductive Biology
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