International Journal of
Reproductive Biomedicine
Tue, Dec 3, 2024
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Volume 22, Issue 1 (January 2024)
IJRM 2024, 22(1): 17-30 |
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Ethics code: IR.SBMU.ACE.1401.013
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Aghajanpour F, Soltani R, Afshar A, Abbaszadeh H, Fadaei Fathabadi F, Moeinian N, et al . Sertoli cell-conditioned medium can improve blood-testis-barrier function and spermatogenesis in azoospermia mice induced by scrotal hyperthermia: An experimental study. IJRM 2024; 22 (1) :17-30
URL: http://ijrm.ir/article-1-3259-en.html
URL: http://ijrm.ir/article-1-3259-en.html
Fakhroddin Aghajanpour1 
, Reza Soltani1 , Azar Afshar1 , Hojjat-Allah Abbaszadeh2 , Fatemeh Fadaei Fathabadi2 , Nafiseh Moeinian2 , Abbas Aliaghaei2 , Ali Dehghani Nejad3 , Reza Mastery Farahani2 , Mohsen Norouzian * 4, Mohammad-Amin Abdollahifar2
, Reza Soltani1 , Azar Afshar1 , Hojjat-Allah Abbaszadeh2 , Fatemeh Fadaei Fathabadi2 , Nafiseh Moeinian2 , Abbas Aliaghaei2 , Ali Dehghani Nejad3 , Reza Mastery Farahani2 , Mohsen Norouzian * 4, Mohammad-Amin Abdollahifar2
1- Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Student Research Committee, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2- Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3- Department of Biology and Anatomical Sciences, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
4- Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. ,norozian93@gmail.com
2- Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3- Department of Biology and Anatomical Sciences, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
4- Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. ,
Abstract: (432 Views)
Background: An increase in the temperature of the testis is associated with damage to the epithelium of seminiferous tubules and disruption of sperm production.
Objective: The current study aimed to investigate the effect of the Sertoli cell-conditioned medium (SCCM) on the blood-testis-barrier associated genes and spermatogenesis process following scrotal hyperthermia.
Materials and Methods: In this experimental study, 40 adult NMRI mice (8 wk, 25-30 gr) were allocated into 4 groups: I) control, II) DMEM (10 μl Dulbecco's Modified Eagle Medium), III) scrotal hyperthermia, and IV) scrotal hyperthermia+SCCM (10 μl SCCM). Hyperthermia was induced by placing the mice scrotum in water at 43°C for 20 min every other day for 10 days. Mice were treated every other day for 5 wk. Then the animals were euthanized, and the tails of epididymis were removed to analyze sperm parameters, testis were taken for stereological assessment, reactive oxygen spices and glutathione levels, and the expression of Ocln, Gja1, Cdh2, and Itgb1.
Results: The results of sperm analysis indicated that SCCM-treated mice significantly increased sperm count and motility and reduced DNA fragmentation. In addition, histological and molecular findings showed that the volume of testicular tissue, the number of germ cells, the glutathione level, and the expression of Ocln, Gja1, Cdh2, and Itgb1 genes were significantly increased in the SCCM-treated mice.
Conclusion: Findings suggest that growth factors of SCCM stimulate the proliferation and differentiation of germ cells through paracrine effects and upregulate the blood-testis-barrier-associated genes in mice subjected to scrotal hyperthermia.
Objective: The current study aimed to investigate the effect of the Sertoli cell-conditioned medium (SCCM) on the blood-testis-barrier associated genes and spermatogenesis process following scrotal hyperthermia.
Materials and Methods: In this experimental study, 40 adult NMRI mice (8 wk, 25-30 gr) were allocated into 4 groups: I) control, II) DMEM (10 μl Dulbecco's Modified Eagle Medium), III) scrotal hyperthermia, and IV) scrotal hyperthermia+SCCM (10 μl SCCM). Hyperthermia was induced by placing the mice scrotum in water at 43°C for 20 min every other day for 10 days. Mice were treated every other day for 5 wk. Then the animals were euthanized, and the tails of epididymis were removed to analyze sperm parameters, testis were taken for stereological assessment, reactive oxygen spices and glutathione levels, and the expression of Ocln, Gja1, Cdh2, and Itgb1.
Results: The results of sperm analysis indicated that SCCM-treated mice significantly increased sperm count and motility and reduced DNA fragmentation. In addition, histological and molecular findings showed that the volume of testicular tissue, the number of germ cells, the glutathione level, and the expression of Ocln, Gja1, Cdh2, and Itgb1 genes were significantly increased in the SCCM-treated mice.
Conclusion: Findings suggest that growth factors of SCCM stimulate the proliferation and differentiation of germ cells through paracrine effects and upregulate the blood-testis-barrier-associated genes in mice subjected to scrotal hyperthermia.
Type of Study: Original Article |
Subject:
Reproductive Biology
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