Volume 22, Issue 7 (July 2024)                   IJRM 2024, 22(7): 553-566 | Back to browse issues page

Ethics code: IR.ARAKU.REC.1400.003


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shariatzadeh S M A, Salmani F, Moghanlo H, Mahmoodi M. Stereological and biochemical effects of thymoquinone on ovarian tissue toxicity induced by silver nanoparticles in NMRI mice: An experimental study. IJRM 2024; 22 (7) :553-566
URL: http://ijrm.ir/article-1-3301-en.html
1- Department of Biology, Faculty of Sciences, Arak University, Arak, Iran. , s-shariatzadeh@araku.ac.ir
2- Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
Abstract:   (1482 Views)
Background: The toxicity of silver nanoparticles (AgNPs) has been proven in the female reproductive system. Thymoquinone (TQ) is a natural antioxidant and bioactive component of Nigella sativa.
Objective: We evaluated the efficacy of TQ on ovarian tissue following toxicity induced by AgNPs in female mice.
Materials and Methods: 24 female NMRI mice (5-6 wk, an average weight of 33 gr) were randomly divided into 4 groups (n = 6/each): control, AgNPs (500 mg/kg, gavage), TQ (2.5 mg/kg, intraperitoneal injection), and TQ+AgNPs. Mice were treated every day for 35 days. Serum levels of malondialdehyde (MDA), total antioxidant capacity (TAC), luteinizing hormone, and follicle-stimulating hormone were measured. The optical disector and stereological techniques were utilized to estimate the follicular count, their volume at different developmental stages, and the structure of ovarian tissue.
Results: In the AgNPs group, the serum concentrations of TAC (p = 0.01), luteinizing hormone (p < 0.001), follicle-stimulating hormone, the volume of corpus luteum (p < 0.001), and the number of follicles decreased significantly compared to the control group. Nevertheless, AgNPs significantly increased the MDA level. In the TQ+AgNPs group compared to the AgNPs group, a significant decrease in MDA level (p < 0.001) and a significant improvement in TAC (p = 0.03), and hormonal levels, the number of primary, preantral, and antral follicles (p = 0.04), and the volume of corpus luteum (p = 0.01) were observed.
Conclusion: TQ improved the number of follicles by reducing oxidative stress and lipid peroxidation in AgNPs-damaged ovarian tissue.
Full-Text [PDF 3407 kb]   (1282 Downloads)    
Type of Study: Original Article | Subject: Reproductive Biology

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb