International Journal of
Reproductive Biomedicine
Sat, Dec 21, 2024
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Volume 22, Issue 10 (October 2024)
IJRM 2024, 22(10): 781-792 |
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Ethics code: IR.IUMS.REC.1402.1191
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Torkashvand H, Shabani R, Artimani T, Pilehvari S, Moghimi M, Mehdizadeh M. Follicular fluid meiosis activating sterol supplementation enhances oocyte maturation and fertilization in a microfluidic system: A lab trial study. IJRM 2024; 22 (10) :781-792
URL: http://ijrm.ir/article-1-3407-en.html
URL: http://ijrm.ir/article-1-3407-en.html
Hossein Torkashvand1 
, Ronak Shabani2 , Tayebe Artimani3 , Shamim Pilehvari3 , Mahdi Moghimi4 , Mehdi Mehdizadeh * 5
, Ronak Shabani2 , Tayebe Artimani3 , Shamim Pilehvari3 , Mahdi Moghimi4 , Mehdi Mehdizadeh * 5
1- Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
2- Reproductive Sciences and Technology Research Center, Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
3- Fertility and Infertility Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
4- School of Mechanical Engineering, Iran University of Science and Technology, Tehran, Iran.
5- Reproductive Sciences and Technology Research Center, Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. ,Mehdizadeh.m@iums.ac.ir
2- Reproductive Sciences and Technology Research Center, Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
3- Fertility and Infertility Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
4- School of Mechanical Engineering, Iran University of Science and Technology, Tehran, Iran.
5- Reproductive Sciences and Technology Research Center, Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. ,
Abstract: (121 Views)
Background: In vitro maturation (IVM) is a promising technique in assisted reproductive technologies, offering benefits such as reducing the risk of ovarian hyperstimulation syndrome.
Objective: This study aimed to evaluate the effects of timed follicular fluid meiosis-activating sterol (FF-MAS) supplementation on the IVM of germinal vesicle oocytes using a dynamic microfluidic system.
Materials and Methods: In this lab trial study, 266 germinal vesicle oocytes were collected from the Infertility Center of Fatemieh hospital, Hamedan, Iran between June 2023 and January 2024. The oocytes were allocated into 3 groups for dynamic microfluidic culture. Each group received culture medium at a flow rate of 0.36 µL/min for 24 hr through inlet A and FF-MAS supplementation through inlet B for 1, 2, and 6 hr. The study evaluated maturation and fertilization rates, embryo development, and mitochondrial status, which was assessed using the JC-1 mitochondrial membrane potential assay.
Results: Maturation rates were significantly higher in the medium-term FF-MAS exposure (MTG) and long-term FF-MAS exposure groups compared to the short-term FF-MAS group (STG) (p < 0.05). Fertilization rates were also higher in the MTG and long-term FF-MAS group compared to the STG (p < 0.05). Embryo formation rates and the proportion of good-quality embryos were higher in the MTG compared to the STG (100% vs. 75%; p = 0.03) and (83.3% vs. 33.3%; p = 0.01), respectively. Mitochondrial peripheral distribution was significantly higher in the MTG than in the STG (p = 0.04).
Conclusion: Optimizing FF-MAS exposure duration enhances IVM efficiency, offering a promising strategy to increase oocyte utilization in in vitro fertilization programs.
Objective: This study aimed to evaluate the effects of timed follicular fluid meiosis-activating sterol (FF-MAS) supplementation on the IVM of germinal vesicle oocytes using a dynamic microfluidic system.
Materials and Methods: In this lab trial study, 266 germinal vesicle oocytes were collected from the Infertility Center of Fatemieh hospital, Hamedan, Iran between June 2023 and January 2024. The oocytes were allocated into 3 groups for dynamic microfluidic culture. Each group received culture medium at a flow rate of 0.36 µL/min for 24 hr through inlet A and FF-MAS supplementation through inlet B for 1, 2, and 6 hr. The study evaluated maturation and fertilization rates, embryo development, and mitochondrial status, which was assessed using the JC-1 mitochondrial membrane potential assay.
Results: Maturation rates were significantly higher in the medium-term FF-MAS exposure (MTG) and long-term FF-MAS exposure groups compared to the short-term FF-MAS group (STG) (p < 0.05). Fertilization rates were also higher in the MTG and long-term FF-MAS group compared to the STG (p < 0.05). Embryo formation rates and the proportion of good-quality embryos were higher in the MTG compared to the STG (100% vs. 75%; p = 0.03) and (83.3% vs. 33.3%; p = 0.01), respectively. Mitochondrial peripheral distribution was significantly higher in the MTG than in the STG (p = 0.04).
Conclusion: Optimizing FF-MAS exposure duration enhances IVM efficiency, offering a promising strategy to increase oocyte utilization in in vitro fertilization programs.
Type of Study: Original Article |
Subject:
Assisted Reproductive Technologies
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