International Journal of
Reproductive Biomedicine
Fri, May 30, 2025
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Volume 23, Issue 3 (March 2025 2025)
IJRM 2025, 23(3): 275-288 |
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Ethics code: IR.IAU.BABOL.REC.1399.032
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Mojibi F, Fattahi E, Jorsaraei S G A, Kazemi S, Gholamitabar Tabari M. Chrysin’s protective effect on the expression of Protamine, Tsga10, Dazl and Akap4 Genes against diazinon toxin in male rats: An experimental study. IJRM 2025; 23 (3) :275-288
URL: http://ijrm.ir/article-1-3465-en.html
URL: http://ijrm.ir/article-1-3465-en.html
Fatemeh Mojibi1 
, Esmail Fattahi *2 , Seyed Gholam Ali Jorsaraei3 , Sohrab Kazemi4 , Maryam Gholamitabar Tabari5
, Esmail Fattahi *2 , Seyed Gholam Ali Jorsaraei3 , Sohrab Kazemi4 , Maryam Gholamitabar Tabari5
1- Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
2- Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran. ,fatahi@iau.ac.ir
3- Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
4- Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
5- Health Reproductive Research Center, Sari Branch, Islamic Azad University, Sari, Iran.
2- Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran. ,
3- Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
4- Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
5- Health Reproductive Research Center, Sari Branch, Islamic Azad University, Sari, Iran.
Abstract: (18 Views)
Background:Environmental pollution with pesticides is a major health problem worldwide that causes toxicity. Chrysin (C) is a flavonoid that reduces the adverse effects caused by diazinon (D).
Objective:To investigate the protective effects of Chrysin on the expression of Protamine, Tsga10(Testis-specific gene antigen 10), Dazl (deleted azoospermia-like gene), and Akap4(A-Kinase anchoring protein 4) genes in the testicular tissue of rats receiving D.
Materials and methods:In this experimental study, 42 Wistar rats(7-8 wk, 180-250 gr) were selected. This study was conducted in 2021 at the Pasteur Institute of Iran, North Amol Research Center. Injections were performed 5 days a week for 4 wk. Random grouping of rats: 1. Control:without any injection. 2. Sham: Injection of 10% Tween 80 solution as D solvent. 3. D: Injection at a concentration of 20 mg/kg/BW. 4 and 5. C with low dose(C10 mg/kg/BW) and high dose(C20 mg/kg/BW). 6 and 7: D with low dose of C (D 20 mg/kg/BW/C10 mg/kg/BW) and D with high dose: (D 20 mg/kg/BW/C20 mg/kg/BW). Finally, the testes were examined in terms of histology and gene expression.
Results: Histological results showed that D improved spermatogenesis after C intervention by reducing germinal cells, diameter, and lumen area. SPSS results showed that D reduced the expression of Dazl(p < 0.0001), Tsga10(p = 0.01), and Protamine(p < 0.0001) genes, but increased the expression of Akap4 gene(p < 0.0001).
Conclusion: D has a negative effect on the expression of meiotic genes in rat testicular tissue,while C greatly reduces the adverse effects caused by D.
Objective:To investigate the protective effects of Chrysin on the expression of Protamine, Tsga10(Testis-specific gene antigen 10), Dazl (deleted azoospermia-like gene), and Akap4(A-Kinase anchoring protein 4) genes in the testicular tissue of rats receiving D.
Materials and methods:In this experimental study, 42 Wistar rats(7-8 wk, 180-250 gr) were selected. This study was conducted in 2021 at the Pasteur Institute of Iran, North Amol Research Center. Injections were performed 5 days a week for 4 wk. Random grouping of rats: 1. Control:without any injection. 2. Sham: Injection of 10% Tween 80 solution as D solvent. 3. D: Injection at a concentration of 20 mg/kg/BW. 4 and 5. C with low dose(C10 mg/kg/BW) and high dose(C20 mg/kg/BW). 6 and 7: D with low dose of C (D 20 mg/kg/BW/C10 mg/kg/BW) and D with high dose: (D 20 mg/kg/BW/C20 mg/kg/BW). Finally, the testes were examined in terms of histology and gene expression.
Results: Histological results showed that D improved spermatogenesis after C intervention by reducing germinal cells, diameter, and lumen area. SPSS results showed that D reduced the expression of Dazl(p < 0.0001), Tsga10(p = 0.01), and Protamine(p < 0.0001) genes, but increased the expression of Akap4 gene(p < 0.0001).
Conclusion: D has a negative effect on the expression of meiotic genes in rat testicular tissue,while C greatly reduces the adverse effects caused by D.
Type of Study: Original Article |
Subject:
Fertility & Infertility
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