Volume 11, Issue 10 (12-2013)                   IJRM 2013, 11(10): 807-0 | Back to browse issues page

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Roshangar L, Soleimani-Rad J, Rashidi B, Mazochian H, Nikzad B, Soleimani Rad S. Ultrastructural and morphometrical study of preimplantation endometrium in superovulated mice treated with progesterone or Sildenafil. IJRM 2013; 11 (10) :807-0
URL: http://ijrm.ir/article-1-354-en.html
Ultrastructural and morphometrical study of preimplantation endometrium in superovulated mice treated with progesterone or Sildenafil
Leila Roshangar1 , Jafar Soleimani-Rad * 2, Bahman Rashidi3 , Hossein Mazochian4 , Behzad Nikzad5 , Sara Soleimani Rad6
1- Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2- Drug Applied Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , Lroshangar@yahoo.com
3- Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4- Medical Education Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5- Department of Neuroscience, Tabriz University, Tabriz, Iran
6- Faculty of medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract:   (2342 Views)
Background: Endometrial development has an important role in blastocyst adhesion and implantation. During IVF cycles, endometrial development is enhanced by progesterone.
Objective: The aim of this study was to compare ultrastructural and morphometrical characteristics of mice uterine endometrium in natural cycle with those in superovulated cycles received progesterone or Sildenafil.
Materials and Methods: In This study, 60 female bulb/c mice were divided into 4 groups: a control and 3 experimental; gonadotropin, gonadotropin+ Sildenafil and gonadotropin+ progesterone. In experimental groups the mice superovulated mated. In the gonadotropin+ progesterone and gonadotropin+ Viagra groups, the mice respectively received 1mg progesterone and 3 mg Sildenafil citrate. Their uterine specimens were prepared for morphometrical and ultrastructural study. Height of the epithelial cells was measured, using motic software. Statistical analysis was performed using ANOVA.
Results: Microscopy revealed that in control group the cells had numerous apical microvilli and the height of the cells was 20.52±2.43 μm. In gonadotropin+ progesterone group, the granules were found in basal and apical portions and cellular height were 17.91±2.78 μm which were significantly shorter than in the control and gonadotropin groups (p<0.001). In this group, the apical membrane also contained pinopodes. In gonadotropin +Sildenafil group, the granules were found in both apical and basal portions and the height of the cells were 17.60±2.49 μm which were significantly shorter than in the control and gonadotropin groups (p<0.001). In this group, pinopodes appeared slightly extensive than the other groups.
Conclusion: It is concluded that superovulatory drugs in mice stimulate endometrial maturation but injection of Sildenafil is nearly more positive.
Keywords: Implantation, Progesterone, Sildenafil, Mice, Uterine
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Type of Study: Original Article |
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