Volume 3, Issue 2 (7-2005)                   IJRM 2005, 3(2): 79-82 | Back to browse issues page

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Karimpor Malekshah A, Esmailnejad Moghaddam A. The effect of culture medium volume on in vitro development of mouse embryos. IJRM 2005; 3 (2) :79-82
URL: http://ijrm.ir/article-1-36-en.html
The effect of culture medium volume on in vitro development of mouse embryos
Abbasali Karimpor Malekshah * , Amir Esmailnejad Moghaddam
Abstract:   (2178 Views)
Background: In the field of mammalian embryo culture, the putative influence of autocrine/ paracrine factor(s), produce by the embryos itself, is under investigation. A smaller medium drop can prevent dilution of this factor(s). Objective: The objective of this study was to examine the effect of culture medium volume on in vitro development of mouse 2-cell embryos. Materials and Methods: The embryos were obtained from female NMRI mice. To evaluate the effect of medium volume, groups of 16-20 late 2-cell embryos were cultured in 2, 5, 10, 20, 50 and 100 �l of drops of Ham�s F10 medium for 72 h. Results: Development to blastocyst stage in 50 and 100 �l of drop were significantly higher than this in any other volume (p<0.001). Almost a similar pattern was also observed for hatched blastocyst formation. However, the total number of cells in blastocysts, developing in different volumes, were not significantly different. Conclusion: These results indicate that the optimal volumes of Ham�s F10 medium for mouse early embryo development are 50 to 100 �l. However, volumes as small as 2 �l can successfully support mouse 2-cell embryo development to blastocyst and hatching stages.
Keywords: Mouse embryo, Culture medium, Incubation volume
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Type of Study: Original Article |
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