Volume 11, Issue 11 (12-2013)                   IJRM 2013, 11(11): 919-0 | Back to browse issues page

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Shapouri F, Saeidi S, Ashrafi Kakhki S, Pouyan O, Amirchaghmaghi E, Aflatoonian R. The expression of TLRs in testicular cancer: A case control study. IJRM 2013; 11 (11) :919-0
URL: http://ijrm.ir/article-1-360-en.html
The expression of TLRs in testicular cancer: A case control study
Farnaz Shapouri1 , Shaghayegh Saeidi1 , Sara Ashrafi Kakhki2 , Omid Pouyan3 , Elham Amirchaghmaghi1 , Reza Aflatoonian * 4
1- Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
2- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3- Department of Urology, Tehran University of Medical Sciences, Tehran, Iran
4- Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , R.Aflatoonian@gmail.com
Abstract:   (2626 Views)
Background: It has been suggested that malfunction of immune system may cause testicular cancer. Recently, our understanding of innate immune system has been expanded, by discovery of “Toll- like receptors” (TLRs). Some studies have shown that polymorphisms of TLR2 and 4 can effect on the risk of cancer. Also, the role of TLRs 3and 9 have been shown in apoptosis of cancer cells and metastasis in animal models.
Objective: Little information is available about the influence of innate immunity on testicular malignancy. Therefore, expression of TLRs 2, 3, 4 and 9 as main components of innate immunity has been investigated in this study.
Materials and Methods: In this case control study, TLRs gene expression was examined by RT-PCR in normal testis and testicular cancer tissues. Real time quantitative PCR (Q-PCR) analysis was used to compare the relative expression of TLRs between the samples.
Results: mRNAs of TLR 2, 3, 4 and 9 were expressed in all normal and cancer samples. Q-PCR reveals that cancer samples had stronger expression of these genes in compared with normal ones.
Conclusion: It seems that the different TLRs expression in testicular cancer cells may contribute to extensive signaling pathways involved in carcinogenesis.
Keywords: TLRs (Toll- like Receptors), Testicular Cancer, Innate Immunity, NF-kappaB
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Type of Study: Original Article |
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