Volume 11, Issue 11 (12-2013)                   IJRM 2013, 11(11): 925-0 | Back to browse issues page

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Nabi A, Khalili M A, Halvaei I, Ghasemzadeh J, Zare E. Seminal bacterial contaminations: Probable factor in unexplained recurrent pregnancy loss. IJRM 2013; 11 (11) :925-0
URL: http://ijrm.ir/article-1-361-en.html
Seminal bacterial contaminations: Probable factor in unexplained recurrent pregnancy loss
Ali Nabi1 , Mohammad Ali Khalili * 2, Iman Halvaei1 , Jalal Ghasemzadeh1 , Ehsan Zare1
1- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Khalili59@hotmail.com
Abstract:   (2969 Views)
Background: It is estimated that about 50% of causes of recurrent pregnancy loss (RPL) cases remain unknown. Sperm factors are suggested to have probable role in cases with RPL.
Objective: The goal was to determine the possible relationship between semen bacterial contaminations with unexplained RPL. Also, the correlation between number of bacterial colony and sperm chromatin condensation was examined.
Materials and Methods: This study consisted of 30 fertile men (group A) and 30 infertile (group B) patients with unknown RPL. Semen collection and analysis were done according to WHO manuals. Sperm count and motility were evaluated by Makler chamber. Eosin-Nigrosin and Papanicolaou staining methods were applied for viability and morphology assessment, respectively. The semen samples from both groups were cultured for aerobic bacteria. Aniline blue (AB) and toluidine blue (TB) staining methods were applied for evaluating sperm chromatin condensation.
Results: The numbers of colonies were significantly higher in group B when compared to group A. Also, S. aureus and E. coli showed significant differences between two groups. Both AB+ and TB+ sperm cells showed significant increase in group B compared to group A. There was a significant negative correlation between colony number and progressive motility (p=0.01), sperm viability (p=0.007). In addition, positive correlations were found between colony number and AB (p=0.001) and TB (p=0.004) as well.
Conclusion: Bacterial contaminations in semen of men from RPL couples had significantly higher levels when compared to fertile controls. Presence of microorganisms in semen may be correlated with irregular sperm parameters and quality.
Keywords: Recurrent embryo loss, Bacteria, Semen
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Type of Study: Original Article |
References
1. Pellati D, Mylonakis I, Bertoloni G, Fiore C, Andrisani A, Ambrosini G, et al. Genital tract infections and infertility. Eur J Obstet Gynecol Reprod Biol 2008; 140: 3-11. [DOI:10.1016/j.ejogrb.2008.03.009]
2. Purvis K, Christiansen E. Infection in the male reproductive tract. Impact, diagnosis and treatment in relation to male infertility. Int J Androl 1993; 16: 1-13. [DOI:10.1111/j.1365-2605.1993.tb01146.x]
3. ASRM. Definitions of infertility and recurrent pregnancy loss: a committee opinion. Fertil Steril 2013; 99: 22.
4. Gupta S, Agarwal A, Banerjee J, Alvarez JG. The role of oxidative stress in spontaneous abortion and recurrent pregnancy loss: a systematic review. Obstet Gynecol Surv 2007; 62: 335-347. [DOI:10.1097/01.ogx.0000261644.89300.df]
5. Puscheck EE, Jeyendran RS. The impact of male factor on recurrent pregnancy loss. Curr Opin Obstet Gynecol 2007; 19: 222-228. [DOI:10.1097/GCO.0b013e32813e3ff0]
6. Nanassy L, Carrell DT. Paternal effects on early embryogenesis. J Exp Clin Assist Reprod 2008; 5: 2. [DOI:10.1186/1743-1050-5-2]
7. Hill JA, Anderson DJ, Polgar K, Abbott AF, Politch JA. Pregnancy: Seminal white blood cells and recurrent abortion. Hum Reprod 1994; 9: 1180-1183. [DOI:10.1093/oxfordjournals.humrep.a138655]
8. Sbracia M, Cozza G, Grasso J, Mastrone M, Scarpellini F. Semen parameters and sperm morphology in men in unexplained recurrent spontaneous abortion, before and during a 3 year follow-up period. Hum Reprod 1996; 11: 117-120. [DOI:10.1093/oxfordjournals.humrep.a019002]
9. Saxena P, Misro MM, Chaki SP, Chopra K, Roy S, Nandan D. Is abnormal sperm function an indicator among couples with recurrent pregnancy loss? Fertil Steril 2008; 90: 1854-1858. [DOI:10.1016/j.fertnstert.2007.09.031]
10. Gil-Villa AM, Cardona-Maya W, Agarwal A, Sharma R, Cadavid Á. Assessment of sperm factors possibly involved in early recurrent pregnancy loss. Fertil Steril 2010; 94: 1465-1472. [DOI:10.1016/j.fertnstert.2009.05.042]
11. Ombelet W, Bosmans E, Janssen M, Cox A, Vlasselaer J, Gyselaers W, et al. Semen parameters in a fertile versus subfertile population: a need for change in the interpretation of semen testing. Hum Reprod 1997; 12: 987-993. [DOI:10.1093/humrep/12.5.987]
12. Talebi A, Vahidi S, Aflatoonian A, Ghasemi N, Ghasemzadeh J, Firoozabadi R, et al. Cytochemical evaluation of sperm chromatin and DNA integrity in couples with unexplained recurrent spontaneous abortions. Andrologia 2012; 44: 462-470. [DOI:10.1111/j.1439-0272.2011.01206.x]
13. Halvaei I, Sadeghipour Roodsari HR, Naghibi Harat Z. Acute Effects of Ruta graveolens L. on Sperm Parameters and DNA Integrity in Rats. J Reprod Infertil 2012; 13: 33-38.
14. Nabi A, Khalili M, Halvaei I, Roodbari F. Prolonged incubation of processed human spermatozoa will increase DNA fragmentation. Andrologia 2013 . DOI: 10.1111/and.12088 available at: http://onlinelibrary. wiley.com/doi/10.1111/and.12088/.
15. WHO. WHO laboratory manual for the Examination and processing of human semen. 5th Ed: Cambridge University Press; 2010.
16. Khalili MA, Mojibian M, Sultan AM. Role of oocyte morphology on fertilization and embryo formation in assisted reproductive techniques. Mid East Fertil Soc J 2005; 10: 72-77.
17. Halvaei I, Khalili MA, Soleimani M, Razi MH. Evaluating the role of first polar body morphology on rates of fertilization and embryo development in ICSI cycles. Int J Fertil Steril 2011; 5: 110-115.
18. Auger J, Mesbah M, Huber C, Dadoune J. Aniline blue staining as a marker of sperm chromatin defects associated with different semen characteristics discriminates between proven fertile and suspected infertile men. Int J Androl 1990; 13: 452-462. [DOI:10.1111/j.1365-2605.1990.tb01052.x]
19. Rosenborg L, Rao K, Björndahlt L, Kvist U, Pousette A, Ákerlof E, et al. Changes in human sperm chromatin stability during preparation for in‐vitro fertilization. Int J Androl 1990; 13: 287-296. [DOI:10.1111/j.1365-2605.1990.tb01034.x]
20. Talebi A, Moein M, Tabibnejad N, Ghasemzadeh J. Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. Andrologia 2008; 40: 245-251. [DOI:10.1111/j.1439-0272.2008.00852.x]
21. Kazerooni T, Asadi N, Jadid L, Kazerooni M, Ghanadi A, Ghaffarpasand F, et al. Evaluation of sperm's chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion. J Assist Reprod Genet 2009; 26: 591-596. [DOI:10.1007/s10815-009-9361-3]
22. Tremellen K. Oxidative stress and male infertility-a clinical perspective. Hum Reprod Update 2008; 14: 243-258. [DOI:10.1093/humupd/dmn004]
23. Villegas J, Schulz M, Soto L. Bacteria induce expression of apoptosis in human spermatozoa. Apoptosis 2005; 10: 105-110. [DOI:10.1007/s10495-005-6065-8]
24. Fujita K, Yokota T, Oguri T, Fujime M, Kitagawa R. In vitro adherence of Staphylococcus saprophyticus, Staphylococcus epidermidis, Staphylococcus haemolyticus, and Staphylococcus aureus to human ureter. Urol Res 1992; 20: 399-402. [DOI:10.1007/BF00294495]
25. Nunez-Calonge R, Caballero P, Redondo C, Baquero F, Martinez-Ferrer M, Meseguer M. Ureaplasma urealyticum reduces motility and induces membrane alterations in human spermatozoa. Hum Reprod 1998; 13: 2756-2761. [DOI:10.1093/humrep/13.10.2756]
26. Prabha V, Sandhu R, Kaur S, Kaur K, Sarwal A, Mavuduru RS, et al. Mechanism of sperm immobilization by Escherichia coli. Adv Urol 2010; 2010: 240268. [DOI:10.1155/2010/240268]
27. Rose B, Scott B. Sperm motility, morphology, hyperactivation, and ionophore-induced acrosome reactions after overnight incubation with mycoplasmas. Fertil Steril 1994; 61: 341-348. [DOI:10.1016/S0015-0282(16)56529-7]
28. Khalili M, Sharifi-Yazdi M. The effect of bacterial infection on the quality of human's spermatozoa. Iran J public Health 2001; 35: 62-67.
29. Khalili M, Pourshafie M, Saifi M, Khalili M. Bacterial infection of the reproductive tract of infertile men in Iran. Mid East Fertil Soc J 2000; 5: 126-131.
30. De Lamirande E, Gagnon C. Reactive oxygen species and human spermatozoa. II. Depletion of adenosine triphosphate plays an important role in the inhibition of sperm motility. J Androl 1991; 13: 379-386.
31. Trum JW, Mol BW, Pannekoek Y, Spanjaard L, Wertheim P, Bleker OP, et al. Value of detecting leukocytospermia in the diagnosis of genital tract infection in subfertile men. Fertil Steril 1998; 70: 315-319. [DOI:10.1016/S0015-0282(98)00163-0]
32. Rybar R, Prinosilova P, Kopecka V, Hlavicova J, Veznik Z, Zajicova A, et al. The effect of bacterial contamination of semen on sperm chromatin integrity and standard semen parameters in men from infertile couples. Andrologia 2012; 44: 410-418. [DOI:10.1111/j.1439-0272.2011.01198.x]
33. Gallegos G, Ramos B, Santiso R, Goyanes V, Gosálvez J, Fernández JL. Sperm DNA fragmentation in infertile men with genitourinary infection by Chlamydia trachomatis and Mycoplasma. Fertil Steril 2008; 90: 328-334. [DOI:10.1016/j.fertnstert.2007.06.035]
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