International Journal of
Reproductive Biomedicine
Wed, Jul 1, 2026
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Volume 24, Issue 5 (May 2026)
IJRM 2026, 24(5): 421-432 |
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Ethics code: IR.UMSHA.REC.1402.227
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Pilehvari S, Ghorbani M, Babalhavaeji H, Talebi-Ghane E, Fazli F. The effect of intratesticular autologous platelet-rich plasma injection on sperm DNA fragmentation in men with oligoasthenoteratozoospermia: An RCT. IJRM 2026; 24 (5) :421-432
URL: http://ijrm.ir/article-1-3640-en.html
URL: http://ijrm.ir/article-1-3640-en.html
Shamim Pilehvari1 
, Marzieh Ghorbani2 , Hooshang Babalhavaeji3 , Elaheh Talebi-Ghane4 , Faezeh Fazli *5
, Marzieh Ghorbani2 , Hooshang Babalhavaeji3 , Elaheh Talebi-Ghane4 , Faezeh Fazli *5
1- Fertility and Infertility Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran. & Clinical Research Development Unit of Fatemieh Hospital, Department of Gynecology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
2- Fertility and Infertility Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran. & Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
3- Fertility and Infertility Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran.
4- Modeling of Noncommunicable Diseases Research Center, Institute of Health Sciences and Technologies, Avicenna Health Research Institute, Hamadan University of Medical Sciences, Hamadan, Iran.
5- Fertility and Infertility Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran. & Clinical Research Development Unit of Fatemieh Hospital, Department of Gynecology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. ,f.fazly@umsha.ac.ir
2- Fertility and Infertility Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran. & Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
3- Fertility and Infertility Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran.
4- Modeling of Noncommunicable Diseases Research Center, Institute of Health Sciences and Technologies, Avicenna Health Research Institute, Hamadan University of Medical Sciences, Hamadan, Iran.
5- Fertility and Infertility Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran. & Clinical Research Development Unit of Fatemieh Hospital, Department of Gynecology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. ,
Abstract: (7 Views)
Background: Oligoasthenoteratozoospermia (OAT) is one of the most common multifactorial causes of male infertility. Platelet-rich plasma (PRP), a biological product, has emerged as a promising regenerative therapy in various fields of medicine, including reproductive health. Therefore, we investigated the effect of PRP administration on sperm DNA fragmentation in OAT men.
Objective: The aim was to evaluate the effect of intratesticular injection of PRP on DNA fragmentation index (DFI) in men with idiopathic OAT and assess its potential as a novel therapeutic intervention to enhance sperm DNA integrity.
Materials and Methods: This randomized controlled trial study involved 160 infertile men diagnosed with OAT without underlying diseases who were referred to the infertility center of Fatemieh Infertility Center, Hamadan, Iran, from July 2023 to July 2024. The participants were randomly assigned to intervention and control groups using a block randomization method. The intervention group received 1-2 cc of PRP in each testicle. Sperm parameters and DFI were measured; a hormonal study was also performed before and after the procedure.
Results: The results revealed a significant difference in concentration (p = 0.030), progressive motility (p < 0.001), DNA fragmentation (p < 0.001), normal morphology (p < 0.001), and also testosterone levels (p < 0.001) between groups after PRP injection.
Conclusion: PRP Injection into the testis appears to be a safe, minimally invasive, and potentially effective therapeutic approach in enhancing sperm quality and reducing DFI in men with OAT.
Objective: The aim was to evaluate the effect of intratesticular injection of PRP on DNA fragmentation index (DFI) in men with idiopathic OAT and assess its potential as a novel therapeutic intervention to enhance sperm DNA integrity.
Materials and Methods: This randomized controlled trial study involved 160 infertile men diagnosed with OAT without underlying diseases who were referred to the infertility center of Fatemieh Infertility Center, Hamadan, Iran, from July 2023 to July 2024. The participants were randomly assigned to intervention and control groups using a block randomization method. The intervention group received 1-2 cc of PRP in each testicle. Sperm parameters and DFI were measured; a hormonal study was also performed before and after the procedure.
Results: The results revealed a significant difference in concentration (p = 0.030), progressive motility (p < 0.001), DNA fragmentation (p < 0.001), normal morphology (p < 0.001), and also testosterone levels (p < 0.001) between groups after PRP injection.
Conclusion: PRP Injection into the testis appears to be a safe, minimally invasive, and potentially effective therapeutic approach in enhancing sperm quality and reducing DFI in men with OAT.
Keywords: Male infertility, Oligoasthenoteratozoospermia, Platelet-rich plasma, Sperm DNA fragmentation.
Type of Study: Original Article |
Subject:
Fertility & Infertility
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