International Journal of
Reproductive Biomedicine
Sun, Dec 22, 2024
[Archive]
Volume 11, Issue 12 (1-2013)
IJRM 2013, 11(12): 1013-0 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Nasrolahi O, Khaneshi F, Rahmani F, Razi M. Honey and metformin ameliorated diabetes-induced damages in testes of rat; correlation with hormonal changes. IJRM 2013; 11 (12) :1013-0
URL: http://ijrm.ir/article-1-369-en.html
URL: http://ijrm.ir/article-1-369-en.html
1- Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran
2- Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran ,f.khaneshi@yahoo.com
3- Department of Comparative Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2- Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran ,
3- Department of Comparative Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abstract: (3172 Views)
Background: The global prevalence of diabetes mellitus is on rise. Diabetes-induced oxidative stress has been known to affect liver, pancreas, kidney and reproductive organs pathologically. Honey is a natural product of bee with antioxidant properties.
Objective: Current study aimed to analyze the protective effects of Metformin (MF) alone and MF+ natural honey co-administration on diabetes-induced histological derangements in testis of rats.
Materials and Methods: Thirty six, mature male Wistar rats were randomly divided into six groups including; control, honey-dosed non-diabetic, diabetes-induced (65 mg/kg, single dose), honey-administrated diabetic (1.0 g/kg/day), Metformin-received diabetic (100 mg/kg/day), Metformin and honey-co-treated diabetic which were followed 40 days. The animals were anesthetized by diethyl ether and the blood samples were collected. The serum levels of testosterone, Insulin, LH and FSH analyzed using antibody enzyme immunoassay method. The testicular tissues were dissected out and underwent to histological analyses.
Results: The biochemical analyses revealed that the diabetes resulted in significantly reduced testosterone (p<0.01), LH and FSH (P<0.01, 0.001) levels in serum. Light microscopic analyses showed remarkable (p<0.01) reduction in seminiferous tubules diameter (STD), spermiogenesis index (SPI) and thickness of the epithelium in the diabetic group versus control and co-treated groups. Simultaneous administration of the honey with MF could fairly up-regulate testosterone, LH and FSH levels. The animals in metformin and honey-treated group exhibited with improved tubules atrophy, elevated spermiogenesis index and germinal epithelium thickness.
Conclusion: Our data indicated that co-administration of Metformin and honey could inhibit the diabetes-induced damages in testicular tissue. Moreover, the simultaneous administration of metformin and honey up-regulated the diabetes-reduced insulin, LH, FSH and testosterone levels.
Objective: Current study aimed to analyze the protective effects of Metformin (MF) alone and MF+ natural honey co-administration on diabetes-induced histological derangements in testis of rats.
Materials and Methods: Thirty six, mature male Wistar rats were randomly divided into six groups including; control, honey-dosed non-diabetic, diabetes-induced (65 mg/kg, single dose), honey-administrated diabetic (1.0 g/kg/day), Metformin-received diabetic (100 mg/kg/day), Metformin and honey-co-treated diabetic which were followed 40 days. The animals were anesthetized by diethyl ether and the blood samples were collected. The serum levels of testosterone, Insulin, LH and FSH analyzed using antibody enzyme immunoassay method. The testicular tissues were dissected out and underwent to histological analyses.
Results: The biochemical analyses revealed that the diabetes resulted in significantly reduced testosterone (p<0.01), LH and FSH (P<0.01, 0.001) levels in serum. Light microscopic analyses showed remarkable (p<0.01) reduction in seminiferous tubules diameter (STD), spermiogenesis index (SPI) and thickness of the epithelium in the diabetic group versus control and co-treated groups. Simultaneous administration of the honey with MF could fairly up-regulate testosterone, LH and FSH levels. The animals in metformin and honey-treated group exhibited with improved tubules atrophy, elevated spermiogenesis index and germinal epithelium thickness.
Conclusion: Our data indicated that co-administration of Metformin and honey could inhibit the diabetes-induced damages in testicular tissue. Moreover, the simultaneous administration of metformin and honey up-regulated the diabetes-reduced insulin, LH, FSH and testosterone levels.
Type of Study: Original Article |
References
1. Erejuwa OO, Sulaiman SA, Wahab MS, Sirajudeen KNS, Salleh MS, Gurtu S. Effect of Glibenclamide alone versus Glibenclamide and Honey on Oxidative Stress in Pancreas of Streptozotocin Induced Diabetic Rats. Int J Appl Res Nat Prod 2011; 4: 1-10.
2. Rösen P, Nawroth PP, King G, Möller W, Tritschler HJ, Packer L. The role of oxidative stress in the onset and progression of diabetes and its complications: asummary of a Congress Series sponsored by UNESCO-MCBN, the American Diabetes Association and the German Diabetes Society. Diabetes Metab Res Rev 2001; 17: 189-212. [DOI:10.1002/dmrr.196]
3. Balasubramanian K, Sivashanmugam P, Thameemdheen S, Govindarajulu P. Effect of diabetes mellitus on epididymal enzymes of adult rats. Indian J Exp Biol 1991; 29: 907-909.
4. Rohrbach DH, Martin GR. Structure of basement membrane in normal and diabetic tissue. Ann NY Acad Sci 1982; 401: 203-211. [DOI:10.1111/j.1749-6632.1982.tb25719.x]
5. Erejuwa OO, Sulaiman SA, AbWahab MS, Sirajudeen KNS, Salleh MS, Gurtu S. Glibenclamide or Metformin Combined with Honey Improves Glycemic Control in Streptozotocin-Induced Diabetic Rats. Int J Biol Sci 2011; 7: 244-252. [DOI:10.7150/ijbs.7.244]
6. Erejuwa OO, Sulaiman SA, Mohd Sab. Honey: A Novel Antioxidant. Molecules 2012; 17: 4400-4423. [DOI:10.3390/molecules17044400]
7. Agarwal A, Prabakaran SA, Said TM. Prevention of oxidative stress injury to sperm. J Androl 2005; 26: 654-660. [DOI:10.2164/jandrol.05016]
8. Gheldof N, Wang XH, Engeseth NJ. Identification and Quantification of Antioxidant Components of Honeys from Various Floral Sources. J Agric Food Chem 2002; 50: 5870-5877. [DOI:10.1021/jf0256135]
9. Erejuwa OO, Sulaiman SA, Wahab MS, Salam SK, Salleh MS, Gurtu S. Hepatoprotective effect of tualang honey supplementation in streptozotocin-induced diabetic rats. Int J Appl Res Nat Prod 2012; 4: 37-41.
10. Erejuwa OO, Sulaiman SA, Wahab MS, Sirajudeen KNS, Salleh MS, Gurtu S. antioxidant protection of Malaysian tualang honey in pancreas of normal and streptozotocin-induced diabetic rats. Ann Endocrinol (Paris) 2011; 71: 291-296. [DOI:10.1016/j.ando.2010.03.003]
11. Mohamed M, Sulaiman SA, Jaafar H, Sirajudeen KN. Antioxidant protective effect of honey in cigarette smoke-induced testicular damage in rats. Int J Mol Sci 2011; 12: 5508-5521. [DOI:10.3390/ijms12095508]
12. Wu MS, Johnston P, Sheu WH, Hollenbeck CB, Jeng CY, Goldfine ID, et al. Effect of metformin on carbohydrate and lipoprotein metabolism in NIDDM patients. Diabetes Care 1990; 13: 1-8. [DOI:10.2337/diacare.13.1.1]
13. Moll E, Veen F, Wely M. The role of metformin in polycystic ovary syndrome: a systematic review. Hum Reprod 2007; 13: 527-537. [DOI:10.1093/humupd/dmm026]
14. Tartarin P, Moison D, Froment P. Metformin exposure affects human and mouse fetal testicular cells. Hum Reprod 2012; 27: 3304-3314 [DOI:10.1093/humrep/des264]
15. Soudmany S, Yuvaraj S, Malini T, Balasubramanian K. Experimental diabetes has adverse effects on the differentiation of ventral prostate during sexual maturation of rats. Anat Rec A Discov Mol Cell Evol Biol 2005; 287: 1281-1289. [DOI:10.1002/ar.a.20250]
16. Khayatnouri MH, Safavi SE, Safarmashaei S, Mikailpourardabili B, Babazadeh D. Effect of Saffron on Histomorphometric Changes of Testicular Tissue in Rat. Am J Anim Vet Sci 2011; 6: 153-159. [DOI:10.3844/ajavsp.2011.153.159]
17. Shetty G, Wilson G, Huhtaniemi I, Shuttlesworth GA, Reissmann T, Meistrich ML. Gonadotropin-releasing hormone analogs stimulate and testosterone inhibits the recovery of spermatogenesis in irradiated rat. Endocrinology 2000; 141: 1735-1745. [DOI:10.1210/endo.141.5.7446]
18. Orth JM, Murray FT, Bardin CW. Ultrastructura Changes in Leydig Cells of Streptozotocin-induced Diabetic Rats. Anat Rec 1979; 195: 415-430. [DOI:10.1002/ar.1091950302]
19. Kühn-Velten N, Waldenburger D, Staib W. Evaluation of steroid biosynthetic lesions inIsolated Leydig Cells from the Testes of Streptozotocin-Diabetic Rats. Diabetologia 1982; 23: 529-533. [DOI:10.1007/BF00254305]
20. Kiani fard D, Hasanzadeh SH, Sadrkhanluo RA, Farshid M. An investigation of ultrastructural changes in cells of seminiferous tubules of testes and alterations in gonadotropic-gonadal hormones of adult male experimentally induced diabetic rats. Urmia Med J 2011; 22: 239-248.
21. Bairyk L, Kumar G, Yeshwanth R. effect of acyclovir on the sperm parameters of albino mice. Indian J Physiol Pharmacol 2009; 53: 327-333.
22. Stefanovic A, Stevuljevic JK, Spasic S, Stanojevic NB, Bujisic N. The influence of obesity on the oxidative stress status and the concentration of leptin in type 2 diabetes mellitus patients. Diabetes Res Clin Pract 2008; 79: 156-163. [DOI:10.1016/j.diabres.2007.07.019]
23. Yang J, Zhang Y, Wang Y, Cui S. Toxiceffects of zearalenone and alpha-zearalenol on the regulation of steroidogenesis and testosterone production in mouse Leydig cells. Toxicol In Vitro 2007; 21: 558-565. [DOI:10.1016/j.tiv.2006.10.013]
24. Said TM, Aziz N, Sharma RK, Lewis-Jones I, Anthony J, Thomas Jr, et al. Novel association between sperm deformity index and oxidative stress-induced DNA damagein infertile male patients. Asian J Androl 2005; 7: 21-126. [DOI:10.1111/j.1745-7262.2005.00022.x]
25. Guneli E, Tugyan K, Ozturk H, Gmustekin M. Effect of melatonin on testicular damage in Streptozotocin-induced diabetic rats. Eur Surg Res 2008; 40: 354-360. [DOI:10.1159/000118032]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
