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Volume 11, Issue 12 (1-2013)
IJRM 2013, 11(12): 1033-0 |
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Dayer D, Nikbakht R, Kadkhodai Elyaderani M. Comparison of leptin concentrations between infertile women with polycystic ovary syndrome and fertile women. IJRM 2013; 11 (12) :1033-0
URL: http://ijrm.ir/article-1-372-en.html
URL: http://ijrm.ir/article-1-372-en.html
1- Department of Laboratory Sciences, Para Medical Faculty, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , dayer86@gmail.com
2- Department of Obstetrics and Gynecology, Fertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3- Department of Biochemistry, Medicine Faculty, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2- Department of Obstetrics and Gynecology, Fertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3- Department of Biochemistry, Medicine Faculty, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Abstract: (1825 Views)
Background: Some studies propose that changes in leptin concentrations (above or under the normal range) result in infertility. Therefore, we investigated serum and follicular fluid leptin concentrations in infertile women with polycystic ovary syndrome (PCOS).
Objective: To study serum and follicular fluid leptin concentrations in infertile women with PCOS.
Materials and Methods: We conducted a case-control study. The case group consisted of 30 infertile women with PCOS who were admitted to the Infertility Department of Imam Khomainy Hospital in Ahvaz, Iran. The control group consisted of 30 healthy fertile women adjusted for age and body mass index (BMI) with the case group. On day 14 of the menstrual cycle, 5 ml of blood was obtained from subjects in both groups. Serum and follicular fluid leptin concentrations were determined by the enzyme linked immunosorbent assay (ELISA). A Biovendor kit was used for the measurement of leptin concentrations. All data were analyzed using statistical package for the social sciences (SPSS) software (version 17.0, Nie, Bent & Hull, USA).
Results: There was a significant correlation between BMI and serum leptin concentrations in both the control (p=0.005, r=0.516) and case groups (p=0.006, r=0.547). In the case group, serum leptin concentrations were consistent with follicular fluid leptin concentrations (p<0.001, r=0.839). Comparison of serum leptin concentrations between the case and control groups revealed no significant difference (p=0.56).
Conclusion: Infertility among women with PCOS was not a consequence of changes in leptin concentrations.
Objective: To study serum and follicular fluid leptin concentrations in infertile women with PCOS.
Materials and Methods: We conducted a case-control study. The case group consisted of 30 infertile women with PCOS who were admitted to the Infertility Department of Imam Khomainy Hospital in Ahvaz, Iran. The control group consisted of 30 healthy fertile women adjusted for age and body mass index (BMI) with the case group. On day 14 of the menstrual cycle, 5 ml of blood was obtained from subjects in both groups. Serum and follicular fluid leptin concentrations were determined by the enzyme linked immunosorbent assay (ELISA). A Biovendor kit was used for the measurement of leptin concentrations. All data were analyzed using statistical package for the social sciences (SPSS) software (version 17.0, Nie, Bent & Hull, USA).
Results: There was a significant correlation between BMI and serum leptin concentrations in both the control (p=0.005, r=0.516) and case groups (p=0.006, r=0.547). In the case group, serum leptin concentrations were consistent with follicular fluid leptin concentrations (p<0.001, r=0.839). Comparison of serum leptin concentrations between the case and control groups revealed no significant difference (p=0.56).
Conclusion: Infertility among women with PCOS was not a consequence of changes in leptin concentrations.
Type of Study: Original Article |
References
1. Vahidi S, Ardalan A, mohammad K. Prevalence of Primary Infertility in the Islamic Republic of Iran in 2004-2005. Asia Pac J Public Health 2009; 21: 287-293. [DOI:10.1177/1010539509336009]
2. Tehrani FR, Simbar M, Tohidi M, Hosseinpanah F, Azizi F. The prevalence of polycystic ovary syndrome in a community sample of Iranian population: Iranian PCOS prevalence study. Reprod Biol Endocrinol 2011; 9: 39. [DOI:10.1186/1477-7827-9-39]
3. Moschos S, Chan JL. Leptin and reproduction: a review. Fertil Steril 2002; 77: 433-444. [DOI:10.1016/S0015-0282(01)03010-2]
4. Sabogal JC, Munoz L. Leptin in obstetrics and gynecology: a review. Obstet Gynecol Surv 2001; 56: 225-230. [DOI:10.1097/00006254-200104000-00023]
5. Chow TK, Phoon MC. Measurement of serum leptin concentrations in university undergraduates by competitive elisa reveals correlations with body mass index and sex. Adv Physiol Educ 2003; 27: 70-77. [DOI:10.1152/advan.00001.2003]
6. Zhong N, Wu XP, Xu ZR, Wang AH, Luo XH, Cao XZ, et al. Relationship of serum leptin with age, body weight, body mass index, and bone mineral density in healthy mainland Chinese women. Clin Chim Acta 2005; 351: 161-168. [DOI:10.1016/j.cccn.2004.09.003]
7. Pehlivanov B, Mitko M. Serum leptin levels correlate with clinical and biochemical indices of insulin resistance in women with polycystic ovary syndrome. Eur J Contraceptreprod Health Care 2009; 14: 153-159. [DOI:10.1080/13625180802549962]
8. Mantzoros CS. Role of leptin in reproduction. Ann NY Acad Sci 2000; 900: 174-183. [DOI:10.1111/j.1749-6632.2000.tb06228.x]
9. Fedorcsak P, Storeng R, Dale PO, Tanbo T, Torjesen P, Urbancsek J, et al. Leptin and leptin binding activity in the preovulatory follicle of polycystic ovary syndrome patients. Scand J Clin Lab Invest 2000; 60: 649-655. [DOI:10.1080/00365510050216376]
10. Lu XP, Wang B, Huang HF. [Expression of leptin mRNA in luteinized granulosa cells and leptin levels in serum and follicular fluid of non-obese infertile patients with polycystic ovary syndrome]. Zhonghua Yi Xue Za Zhi 2005; 85: 88-91. (In Chinese)
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