جمعه 16 مرداد 1405
[Archive]
دوره 24، شماره 6 - ( 3-1405 )
جلد 24 شماره 6 صفحات 0-0 |
برگشت به فهرست نسخه ها
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ghaedrahmati A, Zandi M. Advanced three-dimensional culture technologies in reproductive medicine: A narrative review. IJRM 2026; 24 (6)
URL: http://ijrm.ir/article-1-3768-fa.html
URL: http://ijrm.ir/article-1-3768-fa.html
فناوریهای پیشرفته کشت سهبعدی جنین در پزشکی تولیدمثل: یک مقاله مروری. International Journal of Reproductive BioMedicine. 1405; 24 (6)
چکیده: (14 مشاهده)
پیشرفتهای اخیر در سیستمهای کشت سهبعدی برای فولیکولها، تخمکها و جنینها، نشاندهنده تحولی بنیادین در فناوریهای کمکباروری و زیستشناسی تولیدمثل است. این پلتفرمهای نوین با شبیهسازی دقیقتر محیط فیزیولوژیکی طبیعی در مقایسه با سیستمهای دوبعدی متعارف، مستقیماً به محدودیتهای حیاتی پروتکلهای فعلی تولید آزمایشگاهی جنین میپردازند. این مرور جامع، طیف وسیعی از راهبردهای نوظهور سهبعدی شامل دستگاههای میکروفلوییدیک، شرایط کشت پویا، مدلهای اسفروئیدی/ارگانوئیدی و سیستمهای پیشرفته کشت مشترک با سلولهای سوماتیک و بنیادی پشتیبان را بررسی میکند. شواهد فزاینده نشان میدهد که این فناوریها (با حفظ ساختار سهبعدی فولیکولی و جنینی، بهبود تبادل مواد مغذی و گاز و بازیابی تعاملات حیاتی سلول-سلول و سلول-ماتریکس) به طور قابل توجهی نرخ بلوغ تخمک، شایستگی تکوینی جنین و پیامدهای کلی لقاح آزمایشگاهی را بهبود میبخشند. با این وجود، چالشهایی مانند بهینهسازی وابسته به گونه، مقیاسپذیری، هزینههای بالا و ضرورت استانداردسازی بالینی همچنان موانع مهمی محسوب میشوند. راهکارهای آینده برای غلبه بر این موانع شامل توسعه مواد زیستی هوشمند و پاسخگو به فیزیولوژی، تلفیق فناوریهای پیشرفته مانند هوش مصنوعی و پلتفرمهای ارگان-روی-چیپ، و ایجاد سیستمهای مقرونبهصرفه برای کاربرد گسترده است. این مرور بر پتانسیل تحولآفرین سیستمهای کشت سهبعدی به عنوان یک رویکرد جدید اساسی در تحقیقات تولیدمثلی و درمانهای ناباروری تأکید میکند.
نوع مطالعه: Review article |
فهرست منابع
1. Ferronato GDA, Dos Santos CM, Rosa PMDS, Bridi A, Perecin F, Meirelles FV, et al. Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system. PLoS One 2023; 18: e0284809. [DOI:10.1371/journal.pone.0284809] [PMID] [PMCID]
2. Brison DR. IVF children and healthy aging. Nat Med 2022; 28: 2476-2477. [DOI:10.1038/s41591-022-02098-2] [PMID]
3. Zandi M, Ghaedrahmati A, Stroebech L. The developmental competence of the bovine embryos processed in IVC-stroebech medium vs. mCR2aa medium. Iran J Appl Anim Sci 2024; 14: 11-16.
4. Sartori R, Prata AB, Figueiredo ACS, Sanches BV, Pontes GCS, Viana JHM, et al. Update and overview on assisted reproductive technologies (ARTs) in Brazil. Anim Reprod 2016; 13: 300-312. [DOI:10.21451/1984-3143-AR873]
5. Canovas S, Ross PJ, Kelsey G, Coy P. DNA methylation in embryo development: Epigenetic impact of ART (assisted reproductive technologies). Bioessays 2017; 39: 1700106. [DOI:10.1002/bies.201700106] [PMID]
6. Yusuf AM. Promise of in vitro embryo production technology for improvement of cattle reproductive potential. EAS J Vet Med Sci 2024; 6: 44-58. [DOI:10.36349/easjvms.2024.v06i02.003]
7. Podda A, Dujíčková L, Ariu F, Leoni GG, Izquierdo D, Paramio M-T, et al. Effect of liquid marble 3d culture system on in vitro maturation and embryo development of prepubertal goat oocytes. Animals (Basel) 2025; 15: 188. [DOI:10.3390/ani15020188] [PMID] [PMCID]
8. De Vos M, Smitz J, Thompson JG, Gilchrist RB. The definition of IVM is clear-variations need defining. Hum Reprod 2016; 31: 2411-2415. [DOI:10.1093/humrep/dew208] [PMID]
9. Chavoshinezhad N, Niknafs B. Innovations in 3D ovarian and follicle engineering for fertility preservation and restoration. Mol Bio Rep 2024; 51: 1004. [DOI:10.1007/s11033-024-09783-0] [PMID]
10. Ghaedrahmati A, Mamouei M, Zandi M. Comparison of single-step and sequential embryo culture systems: Replacement of serum with platelet lysate. Gene Cell Tissue 2024; 11: e136416. [DOI:10.5812/gct-146911]
11. Shen P, Xu J, Wang P, Zhao X, Huang B, Wu F, et al. A new three‐dimensional glass scaffold increases the in vitro maturation efficiency of buffalo (Bubalus bubalis) oocyte via remodelling the extracellular matrix and cell connection of cumulus cells. Reprod Domest Anim 2020; 55: 170-180. [DOI:10.1111/rda.13602] [PMID]
12. Junior JPR, Rocha CD, Pires JVG, Alves KA, Marquini GV, Goulart LR. Effect of three-dimensional collagen membrane culture and presence of cumulus cells on maturation of bovine oocytes. JBRA Assist Reprod 2023; 27: 587-593. [DOI:10.5935/1518-0557.20230017] [PMID] [PMCID]
13. Ren H, Zhang Y, Zhang Y, Qiu Y, Chang Q, Yu X, et al. Optimized study of an in vitro 3D culture of preantral follicles in mice. J Vet Sci 2023; 24: e4. [DOI:10.4142/jvs.22223] [PMID] [PMCID]
14. Candelaria JI, Botigelli RC, Guiltinan C, Shikanov A, Denicol AC. Three-dimensional culture in a bioengineered matrix and somatic cell complementation to improve growth and survival of bovine preantral follicles. J Assist Reprod Genet 2025; 42: 1509-1523. [DOI:10.1007/s10815-025-03497-3] [PMID] [PMCID]
15. Ledda S, Idda A, Kelly J, Ariu F, Bogliolo L, Bebbere D. A novel technique for in vitro maturation of sheep oocytes in a liquid marble microbioreactor. J Assist Reprod Genet 2016; 33: 513-518. [DOI:10.1007/s10815-016-0666-8] [PMID] [PMCID]
16. Bebbere D, Nieddu SM, Ariu F, Piras D, Ledda S. 3D liquid marble microbioreactors support in vitro maturation of prepubertal ovine oocytes and affect expression of oocyte-specific factors. Biology (Basel) 2021; 10: 1101. [DOI:10.3390/biology10111101] [PMID] [PMCID]
17. Delaroche L, Besnard L, Ouary V, Bazin F, Cassuto G. Disposables used cumulatively in routine IVF procedures could display toxicity. Hum Reprod 2024; 39: 936-954. [DOI:10.1093/humrep/deae028] [PMID] [PMCID]
18. Bagheri MJ, Valojerdi MR, Salehnia M. Formation of ovarian organoid by co-culture of human endometrial mesenchymal stem cells and mouse oocyte in 3-dimensional culture system. Cytotechnology 2024; 76: 571-584. [DOI:10.1007/s10616-024-00639-w] [PMID] [PMCID]
19. Urzì O, Gasparro R, Costanzo E, De Luca A, Giavaresi G, Fontana S, et al. Three-dimensional cell cultures: The bridge between in vitro and in vivo models. Int J Mol Sci 2023; 24: 12046. [DOI:10.3390/ijms241512046] [PMID] [PMCID]
20. Francés-Herrero E, Juárez-Barber E, Campo H, López-Martínez S, de Miguel-Gómez L, Faus A, et al. Improved models of human endometrial organoids based on hydrogels from decellularized endometrium. J Pers Med 2021; 11: 504. [DOI:10.3390/jpm11060504] [PMID] [PMCID]
21. Wang Y, Liu M, Zhang Y, Liu H, Han L. Recent methods of droplet microfluidics and their applications in spheroids and organoids. Lab Chip 2023; 23: 1080-1096. [DOI:10.1039/D2LC00493C] [PMID]
22. Sargazi Z, Zavareh S, Salehnia M. Differentiation of human endometrial mesenchymal cells to epithelial and stromal cells by seeding on the decellularized endometrial scaffold. In Vitro Cell Dev Biol Anim 2023; 59: 443-454. [DOI:10.1007/s11626-023-00779-x] [PMID]
23. Domnina A, Alekseenko L, Kozhukharova I, Lyublinskaya O, Shorokhova M, Zenin V, et al. Generation of therapeutically potent spheroids from human endometrial mesenchymal stem/stromal cells. J Pers Med 2021; 11: 466. [DOI:10.3390/jpm11060466] [PMID] [PMCID]
24. Zhang W, Cheng Y, Zhang S, Wei R, Zou K. Application of Matrigel in the 3-dimension culture of female germline stem cells. Reprod Biol 2023; 23: 100769. [DOI:10.1016/j.repbio.2023.100769] [PMID]
25. Krotz SP, Robins JC, Ferruccio T-M, Moore R, Steinhoff MM, Morgan JR, et al. In vitro maturation of oocytes via the pre-fabricated self-assembled artificial human ovary. J Assist Reprod Genet 2010; 27: 743-750. [DOI:10.1007/s10815-010-9468-6] [PMID] [PMCID]
26. Jeon MJ, Choi YS, Kim ID, Criswell T, Atala A, Yoo JJ, et al. Engineering functional rat ovarian spheroids using granulosa and theca cells. Reprod Sci 2021; 28: 1697-1708. [DOI:10.1007/s43032-020-00445-7] [PMID]
27. Li X, Zheng M, Xu B, Li D, Shen Y, Nie Y, et al. Generation of offspring-producing 3D ovarian organoids derived from female germline stem cells and their application in toxicological detection. Biomaterials 2021; 279: 121213. [DOI:10.1016/j.biomaterials.2021.121213] [PMID]
28. Luo H, Li X, Tian GG, Li D, Hou C, Ding X, et al. Offspring production of ovarian organoids derived from spermatogonial stem cells by defined factors with chromatin reorganization. J Adv Res 2021; 33: 81-98. [DOI:10.1016/j.jare.2021.03.006] [PMID] [PMCID]
29. Haider S, Beristain AG. Human organoid systems in modeling reproductive tissue development, function, and disease. Hum Reprod 2023; 38; 1449-1463. [DOI:10.1093/humrep/dead085] [PMID]
30. Saadeldin IM, Ehab S, Noreldin AE, Swelum AA-A, Bang S, Kim H, et al. Current strategies using 3D organoids to establish in vitro maternal-embryonic interaction. J Vet Sci 2024; 25: e40. [DOI:10.4142/jvs.24004] [PMID] [PMCID]
31. Kagawa H, Javali A, Khoei HH, Sommer TM, Sestini G, Novatchkova M, et al. Human blastoids model blastocyst development and implantation. Nature 2022; 601: 600-605. [DOI:10.1038/s41586-021-04267-8] [PMID] [PMCID]
32. Kwong J, Chan FL, Wong K-K, Birrer MJ, Archibald KM, Balkwill FR, et al. Inflammatory cytokine tumor necrosis factor α confers precancerous phenotype in an organoid model of normal human ovarian surface epithelial cells. Neoplasia 2009; 11: 529-541. [DOI:10.1593/neo.09112] [PMID] [PMCID]
33. Pors SE, Ramløse M, Nikiforov D, Lundsgaard K, Cheng J, Andersen CY, et al. Initial steps in reconstruction of the human ovary: survival of pre-antral stage follicles in a decellularized human ovarian scaffold. Hum Reprod 2019; 34: 1523-1535. [DOI:10.1093/humrep/dez077] [PMID]
34. Luddi A, Pavone V, Semplici B, Governini L, Criscuoli M, Paccagnini E, et al. Organoids of human endometrium: A powerful in vitro model for the endometrium-embryo cross-talk at the implantation site. Cells 2020; 9: 1121. [DOI:10.3390/cells9051121] [PMID] [PMCID]
35. Combelles CMH, Fissore RA, Albertini DF, Racowsky C. In vitro maturation of human oocytes and cumulus cells using a co-culture three-dimensional collagen gel system. Hum Reprod 2005; 20: 1349-1358. [DOI:10.1093/humrep/deh750] [PMID]
36. Shirazi A, Motaghi E. The in vitro fertilization of ovine oocytes in the presence of oviductal cells and its effect on the expression of zygote arrest 1 (Zar1) and subsequent embryonic development. J Reprod Infertil 2013; 14: 8-16.
37. Taheri Moghadam M, Saki Gh, Nikbakht R, Eftekhari Moghadam AR. Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cells derived from human follicular fluid to oocyte-like cell. Cell Biol Int 2021; 45: 127-139. [DOI:10.1002/cbin.11475] [PMID]
38. Zolfaghar M, Mirzaeian L, Beiki B, Naji T, Moini A, Eftekhari-Yazdi P, et al. Wharton's jelly derived mesenchymal stem cells differentiate into oocyte like cells in vitro by follicular fluid and cumulus cells conditioned medium. Heliyon 2020; 6: e04992. [DOI:10.1016/j.heliyon.2020.e04992] [PMID] [PMCID]
39. Piechota S, Marchante M, Giovannini A, Paulsen B, Potts KS, Rockwell G, et al. Human-induced pluripotent stem cell-derived ovarian support cell co-culture improves oocyte maturation in vitro after abbreviated gonadotropin stimulation. Hum Reprod 2023; 38: 2456-2469. [DOI:10.1093/humrep/dead205] [PMID] [PMCID]
40. Hsuuw Y-D, Su Y-T, Chan W-H, Wu C-C, Tsai Y-C, Chen H-C, et al. Co-culture with adipose mesenchymal stem cells promotes blastocyst formation and gene expression in embryos from aged mice. Regen Ther 2025; 29: 319-327. [DOI:10.1016/j.reth.2025.03.017] [PMID] [PMCID]
41. Amit M, Shariki C, Margulets V, Itskovitz-Eldor J. Feeder layer- and serum-free culture of human embryonic stem cells. Biol Reprod 2004; 70: 837-845. [DOI:10.1095/biolreprod.103.021147] [PMID]
42. de Almeida Monteiro Melo Ferraz M, Ferronato GA. Opportunities involving microfluidics and 3D culture systems to the in vitro embryo production. Anim Reprod 2023; 20: e20230058. [DOI:10.1590/1984-3143-ar2023-0058] [PMID] [PMCID]
43. Xu S, Lei Y, Wei M, Fang Z, Cheng Y, Zhao G, et al. Transparent alumina ceramics‐based microfluidic chip enables on‐chip cryopreservation for mouse oocyte. Adv Funct Mater 2025; 35: 2503296. [DOI:10.1002/adfm.202503296]
44. Wang M, Zhu T, Liu C, Jin L, Fei P, Zhang B. Oviduct-mimicking microfluidic chips decreased the ROS concentration in the in vitro fertilized embryos of CD-1 mice. Biomed Pharmacother 2022; 154: 113567. [DOI:10.1016/j.biopha.2022.113567] [PMID]
45. Angione SL, Oulhen N, Brayboy LM, Tripathi A, Wessel GM. Simple perfusion apparatus for manipulation, tracking, and study of oocytes and embryos. Fertil Steril 2015; 103: 281-290. [DOI:10.1016/j.fertnstert.2014.09.039] [PMID] [PMCID]
46. Karcz A, Van Soom A, Smits K, Van Vlierberghe S, Verplancke R, Pascottini OB, et al. Development of a microfluidic chip powered by EWOD for in vitro manipulation of bovine embryos. Biosensors (Basel) 2023; 13: 419. [DOI:10.3390/bios13040419] [PMID] [PMCID]
47. Ferraz MAMM, Henning HHW, Costa PF, Malda J, Melchels FP, Wubbolts R, et al. Improved bovine embryo production in an oviduct-on-a-chip system: Prevention of poly-spermic fertilization and parthenogenic activation. Lab Chip 2017; 17: 905-916. [DOI:10.1039/C6LC01566B] [PMID]
48. Mancini V, McKeegan PJ, Schrimpe-Rutledge AC, Codreanu SG, Sherrod SD, McLean JA, et al. Probing morphological, genetic and metabolomic changes of in vitro embryo development in a microfluidic device. Biotechnol Prog 2021; 37: e3194. [DOI:10.1002/btpr.3194] [PMID] [PMCID]
49. Zeringue HC, Rutledge JJ, Beebe DJ. Early mammalian embryo development depends on cumulus removal technique. Lab Chip 2005; 5: 86-90. [DOI:10.1039/b316494m] [PMID]
50. Weng L, Lee GY, Liu J, Kapur R, Toth TL, Toner M. On-chip oocyte denudation from cumulus-oocyte complexes for assisted reproductive therapy. Lab Chip 2018; 18: 3892-3902. [DOI:10.1039/C8LC01075G] [PMID] [PMCID]
51. Iwasaki W, Yamanaka K, Sugiyama D, Teshima Y, Briones-Nagata MP, Maeki M, et al. Simple separation of good quality bovine oocytes using a microfluidic device. Sci Rep 2018; 8: 14273. [DOI:10.1038/s41598-018-32687-6] [PMID] [PMCID]
52. Dessouky AH, El-Hussieny H, El-Sherry TM, Parque V, Fath El-Bab AMR. Development of a low-cost microfluidic chip for hyaluronidase-free oocyte denudation in mammals. Biotechnol Notes 2025; 6: 133-142. [DOI:10.1016/j.biotno.2025.03.001] [PMID] [PMCID]
53. Shi J, Tong W, Yu Z, Ran B, Wang L, Chen H, et al. Quantification of elastic modulus variations during zebrafish embryo development using a 3D-printed microfluidic platform. Sens Actuators B Chem 2025; 423: 136691. [DOI:10.1016/j.snb.2024.136691]
54. Guerrero-Sánchez J, Fernández-Blanco G, Lamas-Toranzo I, González-Brusi L, Pérez-Gómez A, Galiano-Cogolludo B, et al. A novel microfluidics-based device achieves automatic cumulus cells removal and optimal embryo development in cattle. Anim Reprod Sci 2025; 279: 107953. [DOI:10.1016/j.anireprosci.2025.107953] [PMID]
55. Alegretti JR, Rocha AMD, Nogueira-de-Souza NC, Kato N, Barros BC, Motta ELA, et al. controlled dynamic microfluidic culture of murine, bovine, and human embryos improves development: Proof-of-concept studies. Cells 2024; 13: 2080. [DOI:10.3390/cells13242080] [PMID] [PMCID]
56. Shen J, Yu Z, Li W, Zhou X. Oocytes vitrification using automated equipment based on microfluidic chip. Ann Biomed Eng 2025; 53: 471-480. [DOI:10.1007/s10439-024-03623-9] [PMID]
57. Liu J, Lee GY, Biggers JD, Toth TL, Toner M. Low cryoprotectant concentration rapid vitrification of mouse oocytes and embryos. Cryobiology 2021; 98: 233-238. [DOI:10.1016/j.cryobiol.2020.10.016] [PMID]
58. Miao S, Guo C, Jiang Z, Wei H-X, Jiang X, Gu J, et al. Development of an open microfluidic platform for oocyte one-stop vitrification with cryotop method. Biosensors (Basel) 2022; 12: 766. [DOI:10.3390/bios12090766] [PMID] [PMCID]
59. Heo YS, Lee H-J, Hassell BA, Irimia D, Toth TL, Elmoazzen H, et al. Controlled loading of cryoprotectants (CPAs) to oocyte with linear and complex CPA profiles on a microfluidic platform. Lab Chip 2011; 11: 3530-3537. [DOI:10.1039/c1lc20377k] [PMID] [PMCID]
60. Lai D, Ding J, Smith GW, Smith GD, Takayama S. Slow and steady cell shrinkage reduces osmotic stress in bovine and murine oocyte and zygote vitrification. Hum Reprod 2014; 30: 37-45. [DOI:10.1093/humrep/deu284] [PMID] [PMCID]
61. Zhan L, Hinnen H, Gopinathan KA, Toner M. Autonomous cryoprotectant loading of the oocyte using microfluidic transistors. Device 2025; 3: 100715. [DOI:10.1016/j.device.2025.100715] [PMID] [PMCID]
62. Barbato V, Genovese V, De Gregorio V, Di Nardo M, Travaglione A, De Napoli L, et al. Dynamic in vitro culture of bovine and human ovarian tissue enhances follicle progression and health. Sci Rep 2023; 13: 11773. [DOI:10.1038/s41598-023-37086-0] [PMID] [PMCID]
63. Wang S, Larina IV. In vivo dynamic 3D imaging of oocytes and embryos in the mouse oviduct. Cell Rep 2021; 36: 109382. [DOI:10.1016/j.celrep.2021.109382] [PMID] [PMCID]
64. Hatekar P, Tsiartas P, Gómez LM, Mateoiu C, Sehic E, Hellström M, et al. Ovarian function and response to gonadotropins after prolonged perfusion of whole ewe ovaries in a bioreactor. J Assist Reprod Genet 2025; 42: 1491-1507. [DOI:10.1007/s10815-025-03432-6] [PMID] [PMCID]
65. Franko R, De Almeida Monteiro Melo Ferraz M. OoTrap: Enhancing oocyte collection and maturation with a field-deployable fluidic device. Lab Chip 2025; 25: 187-200. [DOI:10.1039/D4LC00660G] [PMID]
66. Fragomeni G, De Napoli L, De Gregorio V, Genovese V, Barbato V, Serratore G, et al. Enhanced solute transport and steady mechanical stimulation in a novel dynamic perifusion bioreactor increase the efficiency of the in vitro culture of ovarian cortical tissue strips. Front Bioeng Biotechnol 2024; 12: 1310696. [DOI:10.3389/fbioe.2024.1310696] [PMID] [PMCID]
67. Zhang S, Zheng D, Wu Y, Lin W, Chen Z, Meng L, et al. Simulated microgravity using a rotary culture system compromises the in vitro development of mouse preantral follicles. PloS One 2016; 11: e0151062. [DOI:10.1371/journal.pone.0151062] [PMID] [PMCID]
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
