Volume 3, Issue 2 (7-2005)                   IJRM 2005, 3(2): 74-78 | Back to browse issues page

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Mahmoudi R, Subhani A, Pasbakhsh P, Etesam F, Amiri I, Salehnia M et al . The Effects of cumulus cells on in vitro maturation of mouse germinal vesicle stage oocytes. IJRM 2005; 3 (2) :74-78
URL: http://ijrm.ir/article-1-39-en.html
The Effects of cumulus cells on in vitro maturation of mouse germinal vesicle stage oocytes
Reza Mahmoudi * , Aligholi Subhani , Parichehr Pasbakhsh , Farideh Etesam , Iraj Amiri , Mozhdeh Salehnia , Farid Abolhasani
Abstract:   (3175 Views)
Background: In vitro maturation (IVM) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (IVF). The pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of IVM remains a challenge. Objective: In this study, we investigated the effect of cumulus cells on maturation and fertilization rate of immature oocytes (Germinal vesicle). Materials and Methods: Germinal vesicle (GV) oocytes were recovered from 6-8 weeks old Balb C female mice 48hr after injection of 10 IU pregnant mare serum gonadotropin (PMSG). Collected oocytes were divided into two groups. Group A: GV oocytes without cumulus (denuded oocyte). Group B: GV oocytes with cumulus cells (cumulus-oocyte complex). The oocytes in both groups were cultured in TCM-199 medium in a humidified atmosphere of 5% CO2 in air at 37�C. The maturation, fertilization and developmental rates were recorded after 24hr. Results: Maturation, fertilization and developmental rates in denuded oocytes (DO) were 65.1%, 68.02%, 78.63% respectively, and in cumulus-oocyte complex (COC) were 78.20%, 85.57% and 85.05%, respectively. The maturation, fertilization and developmental rates of COC were significantly higher than those of DO (p<0.05). Conclusion: The results show that cumulus cells have beneficial effects on maturation, fertilization and cleavage rates of mice oocytes.
Keywords: Immature oocyte, In vitro maturation, Mouse, Cumulus cells
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Type of Study: Original Article |
References
1. Child TJ, Abdul-Jalil AK, Gulekli B, Tan SL. In vitro maturation and fertilization of oocytes from unstimulated normal ovaries, polycystic ovaries, and women with polycystic ovary syndrome. Fertile Sterile 2001; 76: 936-942. [DOI:10.1016/S0015-0282(01)02853-9]
2. Cha KY, Chian RC. Maturation in vitro of immature human oocytes for clinical use. Hum Reprod Update 1998; 4: 103-120. [DOI:10.1093/humupd/4.2.103] [PMID]
3. Hardy K, Wright CS, Franks S, Winston RM. In vitro maturation of oocytes. Br Med Bull 2000; 56: 588-602. [DOI:10.1258/0007142001903391] [PMID]
4. Chian RC, Lim JH, Tan SL. State of the ART in in vitro oocyte maturation. Curr Opin Obstet Gynecol 2004; 16: 211-219. [DOI:10.1097/00001703-200406000-00003] [PMID]
5. Moor RM, Dai Y, Lee C, Fulka J. Oocyte maturation and embryonic failure. Hum Reprod Update 1998; 4: 223-236. [DOI:10.1093/humupd/4.3.223] [PMID]
6. Trounson A, Anderiesz C, Jones G. Maturation of human oocytes in vitro and their developmental competence. Reproduction 2001; 121: 51-75. [DOI:10.1530/rep.0.1210051] [PMID]
7. Motlik J. Cytoplasmic aspects of oocyte growth and maturation in mammals. J Reprod Fertile 1989; 38(Suppl.): 17-25.
8. Combelles CM, Cekleniak NA, Racowsky C, Albertini DF. Assessment of nuclear and cytoplasmic maturation in in vitro matured human oocytes. Hum Reprod 2002; 17: 1006-1016. [DOI:10.1093/humrep/17.4.1006] [PMID]
9. Sutton ML, Gilchrist B, Thompson G. Effects of in-vivo and in vitro environments on the metabolism of the cumulus-oocyte complex and its influence on oocyte developmental capacity. Hum Reprod Update 2003; 9: 35-48. [DOI:10.1093/humupd/dmg009] [PMID]
10. Roberts R, Franks S, Hardy K. Culture environment modulates maturation and metabolism of human oocytes. Hum Reprod 2002;17: 2950-2956. [DOI:10.1093/humrep/17.11.2950] [PMID]
11. Albertini F, Combelles M, Benecchi E, Carabatsos MJ. Cellular basis for paracrine regulation of ovarian follicle development. Reproduction 2001; 121: 647-653. [DOI:10.1530/rep.0.1210647] [PMID]
12. Warriach M, Chohan KR. Thickness of cumulus cell layer is a significant factor in meiotic competence of buffalo oocytes. J Vet Sci 2004; 5 (3): 247-51. [DOI:10.4142/jvs.2004.5.3.247] [PMID]
13. Tanghe S, Soom AV, Nauwynck H, Coryn M, Dekruif A. Minireview: function of the cumulus ophorous during oocyte maturation, ovulation and fertilization. Mol. Repord Dev 2002; 61: 414-424. [DOI:10.1002/mrd.10102] [PMID]
14. Janssenswillen C, Nagy ZP, Van Steirteghem A. Maturation of human cumulus-free germinal vesicle-stage oocytes to metaphase II by Coculture with monolayer Vero cells. Hum Reprod 1995; 10: 375-376. [DOI:10.1093/oxfordjournals.humrep.a135947] [PMID]
15. Downs SM. A gap-junction-mediated signal, rather than an external paracrine factor, predominates during meiotic induction in isolated mouse oocytes. Zygote 2001; 9:71-82. [DOI:10.1017/S0967199401001071] [PMID]
16. Eppig JJ, Schultz RM, O'Brien M, Chesnel F. Relationship between the developmental programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Dev Biol 1994; 164: 1-9. [DOI:10.1006/dbio.1994.1175] [PMID]
17. Shimada M, Terada T. FSH and LH induce progesterone production and progesterone receptor synthesis in cumulus cells, a requirement for meiotic resumption in porcine oocytes. Mol Hum Reprod 2002; 8: 612-618. [DOI:10.1093/molehr/8.7.612] [PMID]
18. Xia GL, Kikuchi K, Noguchi J, Izaike Y. Short time priming of pig cumulus-oocyte complexes with FSH and forskolin in the presence of hypoxanthine stimulates cumulus cells to secrete a meiosis-activating substance. Theriogenology 2000; 53: 1807-1815. [DOI:10.1016/S0093-691X(00)00316-2]
19. Motlik J, Nagai T, Kikuchi K. Resumption of meiosis in pig oocytes cultured with cumulus and parietal granulosa cells: The effect of protein synthesis inhibition. J Exp Zool 1991; 259: 386-391. [DOI:10.1002/jez.1402590314] [PMID]
20. Vanderhyden BC, Armstrong, DT. Role of cumulus cells and serum on the in vitro maturation, fertilization and subsequent development of rat oocytes. Biol Reprod 1989; 40: 720-728. [DOI:10.1095/biolreprod40.4.720] [PMID]
21. Isobe N, Terada T. Effect of the factor inhibiting germinal vesicle breakdown on the disruption of gap junctions and cumulus expansion of pig cumulus-oocyte complexes cultured in vitro. Reproduction 2001; 121: 249-257. [DOI:10.1530/rep.0.1210249] [PMID]
22. De Loos, Zeinstra E, Bevers MM. Follicular wall maintains meiotic arrest in bovine oocytes cultured in vitro. Mol Reprod Dev 1994; 39: 162-165. [DOI:10.1002/mrd.1080390207] [PMID]
23. Motta PM, Makabe S, Naguro T, Correr S. Oocyte follicular cells association during development of human ovarian follicle. A study by high resolution scanning and transmission electron microscopy. Arch Histol Cytol 1994; 57: 369-394. [DOI:10.1679/aohc.57.369] [PMID]
24. Nishi Y, Takeshita T, Sato K, Araki T. Change of the mitochondrial distribution in mouse ooplasm during in vitro maturation. J Nippon Med Sch 2003; 70(5): 408-15. [DOI:10.1272/jnms.70.408] [PMID]
25. Aono N, Naganuma T, Abe Y, Hara K, Sasada H, Sato E, Yoshida H. Successful production of blastocysts following ultrarapid vitrification with step-wise equilibriation of germinal vesicle-stage mouse oocytes. J Reprod Dev 2003; 49 (6): 501-506. [DOI:10.1262/jrd.49.501] [PMID]
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