Volume 1, Issue 1 (1-2003)                   IJRM 2003, 1(1): 24-28 | Back to browse issues page

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Farsi M. Evaluation of the Predictive Value of Semen Parameters in Sperm Fertility Potential Using Intracellular Calcium Increase in Response to Progesterone. IJRM 2003; 1 (1) :24-28
URL: http://ijrm.ir/article-1-4-en.html
Evaluation of the Predictive Value of Semen Parameters in Sperm Fertility Potential Using Intracellular Calcium Increase in Response to Progesterone
Mir-Mehrdad Farsi *
Abstract:   (2689 Views)
Background: While traditional semen parameters are of significant clinical value, total fertilization failure in IVF cycles is not uncommon. Sperm function testing such as; Hamster egg penetration test has severed limitations as a clinical test. The aim of this study was to evaluate the predictive value of semen parameters by using of intracellular calcium [Ca2+]I increase in response to progesterone. Materials and Methods: The [Ca2+]i response to progesterone was measured in spermatozoa of 86 patients referring to the Assisted Conception Unit for semen analysis. The patients were divided into 3 groups; according to their semen parameters and measured intracellular [Ca2+]i increasing in response to progesterone . Results: There was no significant correlation between each individual semen parameter and [Ca2+]i elevation in response to the progesterone, but most of the patients in each group had [Ca2+]i increasing as expected based on sperm parameters. However, there were cases in groups 1 and 2 (Normal and IVF) that demonstrated [Ca2+]i increases which were poor or lower than expected. Out of the 22 patients in the normal category, 8 cases had poor response to [Ca2+]I increase and out of the 47 patients in the IVF group, 9 patients were as well. In addition we measured [Ca2+]I increases in 6 fertile donor samples for comparison purposes. Conclusion: [Ca2+]i increase in response to progesterone is related to predicting value of sperm parameters in most cases. However, the response of sperm to progesterone could be different in some cases that are expected in normal or IVF category based on our semen analysis criteria. We suggest that the [Ca2+]i measurements may perfect the sperm fertility potential.
Keywords: Ca2+]i increases, Progesterone, Spermatozoa]
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Type of Study: Original Article |
References
1. Baldi. E., Krausz. C, and Forti. G. (1995) Nonegenomic actions of progesterone on human spermatozoa. Trends Endocrinol Metab 6: 198-205. [DOI:10.1016/1043-2760(95)00083-T]
2. Barrat, C.L.R., Justin C.St John. (1998) Diagnostic tools in male fertility, Hum Reprod 13: 51-61. [DOI:10.1093/humrep/13.suppl_1.51] [PMID]
3. Barrat.C.L.R. Naeeni, M., Clements.C, Cook ID. Clinical value of sperm morphology measurements for in vitro fertility: Comparisons between World Health Organisation (WHO) criteria of 1987 and 1992. Hum Repord 1995; 10: 587 - 593. [DOI:10.1093/oxfordjournals.humrep.a135994] [PMID]
4. Barrat, C.L.R., McLeod, I.D., Dunphy, B.C. et al. prognostic significance of two putative sperm function tests: hypoosmotic swelling and bovine sperm mucus penetration test (penetraK). Hum Reprod 7: 1240-1244. [DOI:10.1093/oxfordjournals.humrep.a137834] [PMID]
5. Bostofte, E., Bagger, P., Michael, A. et al. Fertility prognosis for infertile men: results of follow up study of semen analysis in infertile men from tow different populations evaluated by the Cox regression model. Fertil Steril 1990; 54: 1100-1106. [DOI:10.1016/S0015-0282(16)54012-6]
6. Bray C, Brown JCK, Publicover S, Barratt C.L.R. Progesterone Interaction with sperm plasma membrane, calcium influx and induction of the acrosome reaction. Reprod Med Review 1999; 7: 81-93. Cambridge University Press. [DOI:10.1017/S0962279999000228]
7. De Jonge CJ. The diagnostic significance of the induced acrosome reaction. Reprod Med Rev 1994; 3: 159-78. [DOI:10.1017/S0962279900000855]
8. Kirkman-Brown, J.C, Bray, C., Stewart, P.M. Barratt C.LR and Publicover SJ. Biphasic elevation of [Ca2+] in individual human spermatozoa exposed progesterone. Dev Biol 2000; 222: 326-335. [DOI:10.1006/dbio.2000.9729] [PMID]
9. krausz C, Bonaccorsi L, Maggio P. Luconi M, Criscuoli L. Fuzzi B, Pellegrini S, Forti G, Baldi E.. Two functional assays of sperm responsiveness to progesterone and their predictive values in in vitro fertilization. Human Reprod 1996; 11: 1661-1667. [DOI:10.1093/oxfordjournals.humrep.a019466] [PMID]
10. Krausz, C., Bonaccorsi, L., Luconi, M. Fuzzi B, Criscuoli L, Pellegrini S, Forti G, Baldi E.. intracellular calcium increase and acrosome reaction in response to Progesterone in human spermatozoa are correlated with in vitro fertizalation. Hum Reprod 1995; 10: 120-124. [DOI:10.1093/humrep/10.1.120] [PMID]
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