Volume 11, Issue 6 (9-2013)                   IJRM 2013, 11(6): 459-0 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Sohrabipour S, Jafari A, Kamalinejad M, Sarrafnejd A, Shahrestany T, Sadeghipour H. The role of flaxseed and vitamin E on oxidative stress in prepubertal rats with experimental varicocele: An experimental study. IJRM 2013; 11 (6) :459-0
URL: http://ijrm.ir/article-1-429-en.html
The role of flaxseed and vitamin E on oxidative stress in prepubertal rats with experimental varicocele: An experimental study
Shahla Sohrabipour1 , Adele Jafari2 , Mohamad Kamalinejad3 , Abdolfatah Sarrafnejd4 , Taherah Shahrestany4 , Hamid-Reza Sadeghipour * 5
1- Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
2- Department of Physiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Department of Pharmacogenosy, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Department of Pathobiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran , sadeghipour@tums.ac.ir
Abstract:   (3005 Views)
Background: Prepubertal varicocele has the most devastating effects on the testes. Oxidative stress is the major cause leading to infertility in varicocele. The antioxidant properties of Flaxseed (FS) treatment in some oxidative diseases have been reported.
Objective: This study aimed to evaluate the antioxidant effect of FS in prepubertal rats with experimental varicocele.
Materials and Methods: Forty two male prepubertal rats were divided into 6 groups: the varicocele group were either fed with 10% FS, or with regular diet, or with Vit E, the group with sham operation fed with 10% FS, or had regular diet, and control rats who had not been operated but received regular diet. Varicocele was created by Koksal method. After 6 weeks sperm superoxide anion and H2O2 were evaluated by flowcytometery. Semen total antioxidant capacity (TAC) by Koracevic method and testes malondialdehyde (MDA) by thiobarbituric acid with spectrophotometry was measured.
Results: While superoxide anion and H2O2 were significantly higher in varicocele grop with regular diet (p=0.0001), FS significantly decreased the previously-mentioned parameters (p=0.0001). There were no significant differences for seminal TAC between 6 groups (p=0.07). Left testicular MDA concentration were lower in varicocele or group that were fed with 10% FS compared with other groups (p=0.001).
Conclusion: Reactive oxygen species (ROS) may cause sperm oxidative damage. FS as a fat soluble antioxidant can scavenge intracellular ROS production in varicocele.
Keywords: Varicocele, Flax, Oxidative stress, Antioxidants
Full-Text [PDF 580 kb]   (539 Downloads) |   |   Full-Text (HTML)  (295 Views)  
Type of Study: Original Article |
References
1. Shiraishi K, Takihara H, Matsuyama H. Effects of grade 1 varicocele detected in the pediatric age-group on testicular development. J Pediatric Surg 2009; 44: 1995-1998. [DOI:10.1016/j.jpedsurg.2009.04.008]
2. Razi M, Sadrkhanloo RA, Malekinejad H, Sarrafzadeh-Rezaei F. Testicular biohistochemical alterations following experimental varicocele in rats. Iran J Reprod Med 2012; 10: 209-218.
3. Moein MR, Khalili MA, Davoudi A. The effect of oral administration of Pentoxifylline on sperm motility of asthenozoospermic ejaculates from men with or without testicular varicoceles. Iran J Reprod Med 2005; 3: 25-29.
4. Moein MR, Soleimani M, Tabibnejad N. Reactive oxygen species (ROS) production in seminal fluid correlates with the severity of varicocele in infertile men. Iran J Reprod Med 2008; 6: 65-69.
5. Amirzargar MA, Yavangi M, Basiri A, Hosseini Moghaddam SM, Babbolhavaeji H, Amirzargar N, et al. Comparison of recombinant human follicle stimulating hormone (rhFSH), human chorionic gonadotropin (HCG) and human menopausal gonadotropin (HMG) on semen parameters after varicocelectomy: a randomized clinical trial. Iran J Reprod Med 2012; 10: 441-452.
6. Abdel Aziz MT, Mostafa T, Atta H, Kamal O, Kamel M, Hosni H, et al. Heme oxygenase enzyme activity in seminal plasma of oligoasthenoteratozoospermic males with varicocele. Andrologia 2010; 42: 236-241. [DOI:10.1111/j.1439-0272.2009.00983.x]
7. Cocuzza M, Athayde KS, Alvarenga C, Srougi M, Hallak J. Grade 3 varicocele in fertile men: a different entity. J Urol 2012; 187:1363-1368. [DOI:10.1016/j.juro.2011.11.114]
8. Zini A, Dohle G. Are varicocele associated with increased deoxyribonucleic acid fragmentation?. Fertil Stril 2011; 96: 1283-1286. [DOI:10.1016/j.fertnstert.2011.10.016]
9. Fideleff H, Boquete H, Suarez M, Ruibal G, Sobrado P, Azaretsky M, et al. Controversies in the evolution of paediatric-adolescent varicocele: clinical, biochemical and histological studies. Eur J Endocrinol 2000; 143: 775-781. [DOI:10.1530/eje.0.1430775]
10. Giagulli VA, Carbone MD. Varicocele correction for infertility: which patients to treat? Int J Androl 2010; 34: 236-341. [DOI:10.1111/j.1365-2605.2010.01081.x]
11. Keene D JB, Sajad Y, Rakoczy G, Cervellione RM. Testicular volume and semen parameters in patients aged 12 to 17 years with idiopathic varicocele. J pediatr surg 2012; 47: 383-385. [DOI:10.1016/j.jpedsurg.2011.11.035]
12. Zampieri N, Pellegrino M, Ottolenghi A, Camoglio FS. Effects of bioflavonoids in the management of subclinical varicocele. Pediatr Surg Int 2010; 26: 505-508. [DOI:10.1007/s00383-010-2574-9]
13. Paradiso Galatioto G, Gravina GL, Angelozzi G, Sacchtti A, Innominato PF, Pace G, et al. May antioxidant therapy improve sperm parameters of men with persistent oligospermia after retrograde embolization for varicocele? World J Urol 2008; 26: 97-102. [DOI:10.1007/s00345-007-0218-z]
14. Basch E, Steve Bent M, Collins J, Dacey C, Hammerness P, Harrison M, et al. Flax and Flaxseed Oil (Linum usitatissimum): A Review by the Natural Standard Research Collaboration. J Soc Integ Oncol 2007; 5: 92-105. [DOI:10.2310/7200.2007.005]
15. Adolphe JL, Whiting SJ, Juurlink BH, Thorpe LU, Alcorn J. Health effects with consumption of the flax lignan secoisolariciresinol diglucoside. Br J Nutr 2010; 103: 929-938. [DOI:10.1017/S0007114509992753]
16. Lee J, Krochak R, Blouin A, Kanterakis S, Chatterjee S, Arguiri E, et al. Dietary flaxseed prevents radiation-induced oxidative lung damage, inflammation and fibrosis in a mouse model of thoracic radiation injury. Cancer Biol Ther 2009; 8: 47-53. [DOI:10.4161/cbt.8.1.7092]
17. Kinniry P, Amrani Y, Vachani A, Solomides C, Arguiri E, Workman A, et al. Dietary flaxseed supplementation ameliorates inflammation and oxidative tissue damage in experimental models of acute lung injury in mice. J Nutr 2006; 136: 1545-1551. [DOI:10.1093/jn/136.6.1545]
18. Lee J, Bhora F, Sun J, Cheng G, Arguiri E, Solomides C, et al. Dietary flaxseed enhances antioxidant defenses and is protective in a mouse model of lung ischemia-reperfusion injury. Am J Physiol Lung Cell Mol Physiol 2008; 294: 255-265. [DOI:10.1152/ajplung.00138.2007]
19. Chen J, Hui E, Ip T, Thompson LU. Dietary flaxseed enhances the inhibitory effect of tamoxifen on the growth of estrogen-dependent human breast cancer (mcf-7) in nude mice. Clin Cancer Res 2004; 10: 7703-7711. [DOI:10.1158/1078-0432.CCR-04-1130]
20. Hosseinian FS, Muir AD, Westcott ND, Krol ES. AAPH-mediated antioxidant reactions of secoisolariciresinol and SDG. Organ Biomol Chem 2007; 5: 644-654. [DOI:10.1039/b617426d]
21. Hajiani M, Golestani A, Shariftabrizi A, Rastegar R, Payabvash S, Hassanzadeh A, et al. Dose-dependent modulation of systemic lipid peroxidation and activity of anti-oxidant enzymes by vitamin E in the rat. Redox Report 2008; 13: 60-66. [DOI:10.1179/135100008X259114]
22. Chen J, Tan KP, Ward WE, Thompson LU. Exposure to flaxseed or its purified lignan during suckling inhibits chemically induced rat mammary tumorigenesis. Exp Biol Med 2003; 228: 951-958. [DOI:10.1177/153537020322800811]
23. Christofidou-Solomidou M, Tyagi S, Tan KS, Hagan S, Pietrofesa R, Dukes F, et al. Dietary flaxseed administered post thoracic radiation treatment improves survival and mitigates radiation-induced pneumonopathy in mice. BMC Cancer 2011; 11: 269-284. [DOI:10.1186/1471-2407-11-269]
24. Razi SS, Latif MJ, Li X, Afthinos JN, Ippagunta N, Schwartz G, et al. Dietary Flaxseed Protects Against Lung Ischemia Reperfusion Injury Via Inhibition of Apoptosis and Inflammation in a Murine Model. J Surg Res 2011; 171:113-121. [DOI:10.1016/j.jss.2011.06.017]
25. Koksal IT, Tefekli A, Usta M, Erol H, Abbasoqlu S, Kadioqlu A. The role of reactive oxygen species in testicular dysfunction associated with varicocele. BJU 2000; 86: 261-265. [DOI:10.1046/j.1464-410X.2000.00755.x]
26. Jafari A, Zahmatkesh M, Sadeghipour H, Kajbafzadeh A, Sarrafnejd A, Shahrestany T, et al. Flow Cytometric Evaluation of Sperm Superoxide Anion Production in Rats With Experimental Varicocele. Urology 2010; 75: 217-222. [DOI:10.1016/j.urology.2009.03.040]
27. Guthrie H, Welch G. Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry. J Anim Sci 2006; 84: 2089-2100. [DOI:10.2527/jas.2005-766]
28. Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 2001; 54: 356-361. [DOI:10.1136/jcp.54.5.356]
29. Esterbauer H, Cheeseman K. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol 1990; 186: 407-421. [DOI:10.1016/0076-6879(90)86134-H]
30. Rhee Y, Brunt A. Flaxseed supplementation improved insulin resistance in obese glucose intolerant people: a randomized crossover design. Nutr J 2011; 10: 44-49. [DOI:10.1186/1475-2891-10-44]
31. MacDonald-Wicks LK, Garg ML. Modulation of carbon tetrachloride-induced oxidative stress by dietary fat in rats. J Nutr Biochem 2002; 13: 87-95. [DOI:10.1016/S0955-2863(01)00199-1]
32. Salama N, Bergh A, Damber JE. The changes in testicular vascular permeability during progression of the experimental varicocele. Eur Urol 2003; 43: 84-91. [DOI:10.1016/S0302-2838(02)00501-8]
33. Elsayed NM, Mustafa MG, Mead JF. Increased vitamin E content in the lung after ozone exposure: A possible mobilization in response to oxidative stress. Arch Biochem Biophys 1990; 282: 263-269. [DOI:10.1016/0003-9861(90)90115-F]
34. Smith R, Kaune H, Parodi D, Madariaga M, Rios R, Morales I, et al. Increased sperm DNA damage in patients with varicocele: relationship with seminal oxidative stress. Hum Reprod 2006; 21: 986-993. [DOI:10.1093/humrep/dei429]
35. Naqshbandi A, Khan W, Rizwan S, Khan F. Studies on the protective effect of flaxseed oil on cisplatin-induced hepatotoxicity. Hum Exp Toxicol 2012; 31: 364-375. [DOI:10.1177/0960327111432502]
36. Agarwal A, Sharma R, Desai N ,Prabakaran S, Tavares A, Sabaneqh E. Role of oxidative stress in pathogenesis of varicocele and infertility. Urology 2009; 73: 461-469. [DOI:10.1016/j.urology.2008.07.053]
37. Nesbitt PD-Lam Y, Thompson LU. Human metabolism of mammalian lignan precursors in raw and processed flaxseed. Am J Clin Nutr 1999; 69: 549-555. [DOI:10.1093/ajcn/69.3.549]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb