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Salehnia M, Arianmanesh M, Beigi M. The impact of ovarian stimulation on mouse endometrium: a morphometrical study. IJRM 2006; 4 (1) :7-11
URL: http://ijrm.ir/article-1-45-en.html
The impact of ovarian stimulation on mouse endometrium: a morphometrical study
Mojdeh Salehnia * 1, Mitra Arianmanesh2 , Mandana Beigi2
1- Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran , mogdeh@dr.com
2- Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran
Abstract:   (2641 Views)
Background: The preparation of endometrium for embryo reception is dependent on the ovarian hormones, which are affected by ovarian hyperstimulation procedure.
Objective: The aim of this study was to evaluate the changes in morphometrical indices of endometrium by the daily injection of progesterone after mouse ovarian induction.
Materials and Methods: Adult virgin female mice were selected and divided into control and experimental groups. Experimental groups were superovulated using human menopasual gonadotropic hormone (HMG), and human chorionic gonadotropic hormone (HCG), then they, were subdivided into two groups, which one group was also injected daily by progesterone. All control and hyperstimulated groups were rendered pseudopregnant by cervical stimulation. Three and four days after the HCG injection, the samples of uterine horns were aparted and processed for light microscopic studies.
Results: Our results showed that in the progesterone-injected group, the height of surface and glandular epithelium was decreased on day three (17.6±3.55, 10.02±2.6) and day four (16.9±4.24, 1.6±0.84) respectively, and it had low columnar morphology in comparison with the hyperstimulated and control groups. Also the intercellular spaces of stroma in progesterone-injected group were narrower than these in the other groups.
Conclusion: Ovarian hyperstimulation followed by progesterone injection alter the morphometrical indices of surface and glandular epithelium of endometrium, which could affect on its receptivity.
Keywords: Endometrium, Morphometry, Ovarian stimulation, Progesterone
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Type of Study: Original Article |
References
1. Leesey BA. The role of the endometrium during embryo implantation. Hum Reprod 2001; 15, 36-50.
2. Nikas G. Endometrial receptivity: changes in cell- surface morphology. Semin Repord Med 2000; 18, 229-235. [DOI:10.1055/s-2000-12561]
3. Creus M, Ordi J, Fabregues F, Casamitjana R, Carmona F, Cardesa A, et al. The effect of different hormone therapies on integrin expression and pinopode formation in the human endometrium: a controlled study. Hum Reprod 2003; 18, 683-693. [DOI:10.1093/humrep/deg177]
4. Nikas G, Makrigiannakis A. Endometrial pinopodes and uterine receptivity. Ann N K Acad Sci 2003; 997, 120-123. [DOI:10.1196/annals.1290.042]
5. Murphy CR. The plasma membrane transformation: a key concept in uterine receptivity. Rep Med Rev 2001; 9, 197-208. [DOI:10.1017/S0962279901000321]
6. Hewitt SC, Korach KS. Progesterone action and responses in the αERKO mouse. Steroid 2000; 65, 551-557. [DOI:10.1016/S0039-128X(00)00113-6]
7. Sengupta J, Ghosh D. Role of peri-implasntation stage endometrium-embryo interaction in the primate. Steroid 2000; 45, 753-762. [DOI:10.1016/S0039-128X(00)00191-4]
8. Bourgain C, Devroey P. The endometrium in stimulated cycles for IVF. Hum Reprod Update 2003; 9, 515-522. [DOI:10.1093/humupd/dmg045]
9. Fossum GT, Davidson A, Paulson RJ. Ovarian hyperstimulation inhibits embryo implantation in the mouse. In Vitro Fert Embryo Transfer 1989; 6, 7-10. [DOI:10.1007/BF01134574]
10. Basir GH, Wai-sum O, Hung Yu Ng E, Chung Ho P. Morphometric analysis of prei-implantation endometrium in patients having excessively high oestradiol concentration after ovarian stimulation. Hum Rreprod 2002; 16, 435-440. [DOI:10.1093/humrep/16.3.435]
11. Ertzeid G, Storeng R. The impact of ovarion stimulation on implantation and fetal development in mice. Hum Reprod 2001; 16, 221-225. [DOI:10.1093/humrep/16.2.221]
12. Kramer B, Stein BA, Van der Walt LA. Exogenous gonadotropins-serum oestrogen and progesterone and the effect on endometrial morphology in the rat. J Anat 1990; 173, 177-189.
13. Biegy M, Salehnia M, Tiraihi T. Delayed decidulaization and ultrastructural changes of mouse endometrium after mouse ovarian hyperstimulation at the implantation time. Middle East Fertil Soc J 2003; 8, 229-234.
14. Pellicer A, Valbuena D, Cano F, Remohi J, Simon C. Lower implantation rates in high responders evidence for an altered endocrine milieu during the implantation period. Fertil Steril 1996; 65, 1190-1195. [DOI:10.1016/S0015-0282(16)58337-X]
15. Thomas K, Thomson AJ, Sephton V, Cowan C, Wood S, Vince G, et al. The effect of gonadotrophic stimulation on integrin expression in the endometrium. Hum Reprod 2002; 17, 63-68. [DOI:10.1093/humrep/17.1.63]
16. Murdoch RN, Kay DH, Cross M. Activity and subcellular distribution of mouse uterine alkaline phosphatase during pregnancy and pseudopregnancy. J Reprod Fertil 1978; 54, 293-300. [DOI:10.1530/jrf.0.0540293]
17. Miller BG, Delayed interactions between progesterone a low doses of 17 β-estradiol in the mouse uterus. Endocrinol 1979; 104, 26-33. [DOI:10.1210/endo-104-1-26]
18. Stein B, Kramer B. The effectof exogenous gonadotropichormones on the endometrium of the rat. H Anat 1989; 164: 123-140.
19. Risek B, Klier F G, Phillips A, Hahn D W, Gilula N B. Gap junction regulation in the uterus and ovaries of immature rats by estrogen and progesterone. J Cell Sci 1995; 108, 1017-1032.
20. Dursun A, Sendag F, Terek M C, Yilmaz H, Oztekin K, Baka M, Tanyalcin T. Morphometric changes in the endometrium and serum leptin levels during the implantation period of the embryo in the rat in response to exogenous ovarian stimulation. Fertil Steril 2004; 82 1121-1126. [DOI:10.1016/j.fertnstert.2004.04.039]
21. Kramer B. Changes in vascular permeability and decidoma formation during the peri-implantation period of the rat in responsee to exogenous gonadotropins. Anat Record 1997; 247, 20-24. https://doi.org/10.1002/(SICI)1097-0185(199701)247:1<20::AID-AR3>3.0.CO;2-J [DOI:10.1002/(SICI)1097-0185(199701)247:13.0.CO;2-J]
22. McRae AC, Heap PB. Uterine vascular permeability, blood flow and extracellular fluid space during implantation in rates. J Reprod Fertil 1988; 82, 617-625. [DOI:10.1530/jrf.0.0820617]
23. Kolb BA, Najmabadi S, Paulson RJ. Ultrastructural characteristics of luteal phase endometrium in patients undergoing controlled ovarian hyperstimulation. Fertil Steril 1997; 67, 625-630. [DOI:10.1016/S0015-0282(97)81356-8]
24. Emadi M, Salehnia M. The morphological expression of endometrial pinopodes during implantation in mice after ovarian stimulation and progesterone injection. Yakhteh Med J 2004; 5, 140-145.
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