Volume 11, Issue 9 (12-2013)                   IJRM 2013, 11(9): 747-0 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hoseinzadeh-Sani S K, Barati F, Khaksary Mahabady M. The effects of ex vivo cold-storage on cryopreservation of the goat (Caprus hircus) epididymal sperm. IJRM 2013; 11 (9) :747-0
URL: http://ijrm.ir/article-1-462-en.html
1- Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2- Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran , fabrtir@yahoo.com
3- Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:   (2279 Views)
Background: There are many studies focused on long or short storage time of epididymal sperm (EPS) of different species. There are limited studies on preservation or cryopreservation of the domestic goat EPS.
Objective: The aim of the present study was to evaluate the effects of ex vivo cold-storage on freezing of EPS from goat (Capra hircus).
Materials and Methods: In a split-plot design the caprine testes-epididymides (40 pairs) were divided to 4 storage-time groups equally (0, 24, 48 and 72 h), then subjected to cryopreservation using Bioxell. Sperm parameters were analyzed before and after freezing.
Results: Duration of cold-storage as well as freezing at all storage-time points reduced sperm viability and progressive motility while increased sperm tail abnormalities (p<0.0001). Freezing reduced the percentage of cytoplasmic droplets (p<0.0001). The percentage of detached heads was increased at all storage-time points following freezing (p=0.0019), except at time 0 h.
Conclusion: It can be concluded that cold storage [in refrigerator (4oC) for 72 h] of epididymides efficiently protected the goat EPS in terms of progressive motility and viability. However, cold-storage may not protect the goat EPS against cryopreservation with Bioxell.
Full-Text [PDF 164 kb]   (750 Downloads) |   |   Full-Text (HTML)  (409 Views)  
Type of Study: Original Article |

1. Fernández-Santos MR, Soler AJ, Ramón M, Ros-Santaella JL, Maroto-Morales A, García-Álvarez O, et al. Effect of post-mortem time on post-thaw characteristics of Spanish ibex (Capra pyrenaica) spermatozoa. Anim Reprod Sci 2011; 129: 56-66. [DOI:10.1016/j.anireprosci.2011.09.011]
2. Azambuja R, Badalotti M, Teloken C, Michelon J, Petracco A. Successful birth after injection of frozen human oocytes with frozen epididymal spermatozoa. Reprod Biomed Online 2005; 11: 449-451. [DOI:10.1016/S1472-6483(10)61138-8]
3. Talebi AR, Khalili MA, Nahangi H, Abbasi A, Anvari M. Evaluation of epididymal necrospermia following experimental chronic spinal cord injury in rat. Iran J Reprod Med 2007; 5: 171-176.
4. Dehghan MH, Martin T, Dehghanan R. Antifertility effect of Iranian neem seed alcoholic extract on epididymal sperm of mice. Iran J Reprod Med 2005; 2: 83-89.
5. Barati F, Papahn AA, Afrough M, Barati M. Effects of Tyrode's solution osmolarities and milk on bull sperm storage above zero temperatures. Iran J Reprod Med 2011; 9: 25-30.
6. Ganan N, Gomendio M, Roldan ER. Effect of storage of domestic cat (Felis catus) epididymides at 5 degrees C on sperm quality and cryopreservation. Theriogenology 2009; 72: 1268-1277. [DOI:10.1016/j.theriogenology.2009.07.023]
7. Barati F, Khaksary mahabady M, Mohammadi GhA. Cryopreservation of in situ cool stored buffalo (Bubalus bubalis) epididymal sperm. Iran J Vet Res 2009; 10: 339-345.
8. Hallak J, Mahran A, Chae J, Agarwal A. Poor semen quality from patients with malignancies does not rule out sperm banking. Urol Res 2000; 28: 281-284. [DOI:10.1007/s002400000129]
9. Kaabi M, Paz P, Alvarez M, Anel E, Boixo JC, Rouissi H, et al. Effect of epididymis handling conditions on the quality of ram spermatozoa recovered post-mortem. Theriogenology 2003; 60: 1249-1259. [DOI:10.1016/S0093-691X(03)00139-0]
10. Ehling C, Rath D, Struckmann C, Frenzel A, Schindler L, Niemann H. Utilization of frozen-thawed epididymal ram semen to preserve genetic diversity in Scrapie susceptible sheep breeds. Theriogenology 2006; 66: 2160-2164. [DOI:10.1016/j.theriogenology.2006.07.003]
11. Malo C, Gil L, Cano R, Martinez F, Gale I. Antioxidant effect of rosemary (Rosmarinus officinalis) on boar epididymal spermatozoa during cryopreservation. Theriogenology 2011; 75: 1735-1741. [DOI:10.1016/j.theriogenology.2011.01.013]
12. Datta U, Chandra Sekar M, Hembram ML, Dasgupta R. Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10oC with electrolyte free medium. J Assist Reprod Genet 2009; 26: 467-473. [DOI:10.1007/s10815-009-9344-4]
13. Herold FC, Aurich JE, Gerber D. Epididymal sperm from the African buffalo (Syncerus caffer) can be frozen successfully with AndroMed® and with Triladyl™ but the addition of bovine seminal plasma is detrimental. Theriogenology 2004; 61: 715-724. [DOI:10.1016/S0093-691X(03)00256-5]
14. Axnér E, Hermansson U, Linde-Forsberg C. The effect of Equex STM paste and sperm morphology on post-thaw survival of cat epididymal spermatozoa. Anim Reprod Sci 2004; 84: 179-191. [DOI:10.1016/j.anireprosci.2003.11.003]
15. Morton KM, Bathgate R, Evans G, Maxwell WM. Cryopreservation of epididymal alpaca (Vicugna pacos) sperm: a comparison of citrate-, Tris- and lactose-based diluents and pellets and straws. Reprod Fertil Dev 2007; 19: 792-796. [DOI:10.1071/RD07049]
16. Graham JK. Effect of seminal plasma on the motility of epididymal and ejaculated spermatozoa of the ram and bull during the cryopreservation process. Theriogenology 1994; 41: 1151-1162. [DOI:10.1016/S0093-691X(05)80037-8]
17. Herold FC, de Haas K, Colenbrander B, Gerber D. Comparison of equilibration times when freezing epididymal sperm from African buffalo (Syncerus caffer) using Triladyl™ or AndroMed®. Theriogenology 2006; 66: 1123-1130. [DOI:10.1016/j.theriogenology.2006.03.007]
18. SAS Institute Inc. SAS/STAT 9.1 Usere's guide. Cary NC; SAS Institue Inc 2004: 1731-1907.
19. Saragusty J, Gacitua H, King R, Arav A. Post-mortem semen cryopreservation and characterization in two different endangered gazelle species (Gazella gazella and Gazella dorcas) and one subspecies (Gazella gazelle acaiae). Theriogenology 2006; 66: 775-784. [DOI:10.1016/j.theriogenology.2006.01.055]
20. Hori T, Uehara Y, Kawakami E, Tsutsui T. Influence of the time between removal and cooling of the canine epididymis on post-thaw caudal epididymal sperm quality. J Vet Med Sci 2009; 71: 811-815. [DOI:10.1292/jvms.71.811]
21. Blash S, Melican D, Gavin W. Cryopreservation of epididymal sperm obtained at necropsy from goats. Theriogenology 2000; 54: 899-905. [DOI:10.1016/S0093-691X(00)00400-3]
22. Kundu CN, Chakrabarty J, Dutta P, Bhattacharyya D, Ghosh A, Majumder GC. Effect of dextrans on cryopreservation of goat cauda epididymal spermatozoa using a chemically defined medium. Reproduction 2002; 123: 907-913. [DOI:10.1530/rep.0.1230907]
23. Kundu CN, Chakraborty J, Dutta P, Bhattacharyya D, Ghosh A, Majumder GC. Development of a simple sperm cryopreservation model using a chemically defined medium and goat cauda epididymal spermatozoa. Cryobiology 2000; 40: 117-125. [DOI:10.1006/cryo.2000.2230]
24. Kundu CN, Das K, Majumder GC. Effect of amino acids on goat cauda epididymal sperm cryopreservation using a chemically defined model system. Cryobiology 2001; 42: 21-27. [DOI:10.1006/cryo.2001.2296]
25. Santiago-Moreno J, Toledano-Díaz A, Pulido-Pastor A, Dorado J, Gómez-Brunet A, López-Sebastián A. Effect of egg yolk concentration on cryopreserving Spanish ibex (Capra pyrenaica) epididymal spermatozoa. Theriogenology 2006; 66: 1219-1226. [DOI:10.1016/j.theriogenology.2006.03.031]
26. Santiago-Moreno J, Toledano-Diaz A, Dorado J, Pulido-Pastor A, Coloma MA, Lopez-Sebastian A. Recovery and cryopreservation of Spanish ibex epididymal spermatozoa. Arch Androl 2007; 53: 309-316. [DOI:10.1080/01485010701730674]
27. Yu WJ, Lee BJ, Nam SY, Ahn B, Hong JT, Do JC, et al. Reproductive disorders in pubertal and adult phase of the male rats exposed to vinclozolin during puberty. J Vet Med Sci 2004; 66: 847-853. [DOI:10.1292/jvms.66.847]
28. Chandiran IS, Vrushabendra Swamy BM, Kumar BP, Narayanan V, Kumar SV, Muralidharan P, Studies on spermatotoxic effect of ethanolic extract of root of "Caesalpinia digyna (Rottler)". Pharmacol Online 2008; 2: 790-795.
29. Boockfor FR, Blake CA. Chronic administration of 4-tert-octylphenol to adult male rats causes shrinkage of the testes and male accessory sex organs, disrupts spermatogenesis, and increases the incidence of sperm deformities. Biol Reprod 1997; 57: 267-277. [DOI:10.1095/biolreprod57.2.267]
30. Baccetti B, Capitani S, Collodel G, Di Cairano G, Gambera L, Moretti E, et al. Genetic sperm defects and consanguinity. Hum Reprod 2001; 16: 1365-1371. [DOI:10.1093/humrep/16.7.1365]
31. Collodel G, Moretti E. Sperm morphology and aneuploidies: Defects of supposed genetic origin. Andrologia 2006; 38: 208-215. [DOI:10.1111/j.1439-0272.2006.00742.x]
32. Pant HC, Mittal AK, Kasiraj R, Prabhakar JH, Misra AK. Abnormal detached heads: A characteristic morphological abnormality in spermatozoa of Holstein Friesian x Sahiwal crossbred bulls. Indian J Anim Sci 2002; 72: 316-318.
33. Barth AD. Sperm accumulation in the ampullae and cauda epididymides of bulls. Anim Reprod Sci 2007; 102: 238-246. [DOI:10.1016/j.anireprosci.2006.11.005]
34. Young RJ. Cross-link formation at the head-tail junction of mammalian spermatozoa during aging is dependent on sperm motility. Arch Androl 1985; 14: 15-19. [DOI:10.3109/01485018508987273]
35. Nöthling JO, Volkmann DH. Case report Dilatation of the ampullae and an increased incidence of loose sperm heads after bilateral vesiculectomy in a bull. Reprod Domes Anim 1997; 32: 321-324. [DOI:10.1111/j.1439-0531.1997.tb01303.x]
36. Cooper TG. The epididymis, cytoplasmic droplets and male fertility. Asian J Androl 2011; 13: 130-138. [DOI:10.1038/aja.2010.97]

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb