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Reproductive Biomedicine
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Volume 12, Issue 11 (12-2014)
IJRM 2014, 12(11): 765-0 |
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Mazaheri M, Shahdadi V, Nazari Boron A. Molecullar and biochemical effect of alcohlic extract of Alpinia galanga on rat spermatogenesis process. IJRM 2014; 12 (11) :765-0
URL: http://ijrm.ir/article-1-488-en.html
URL: http://ijrm.ir/article-1-488-en.html
Abstract: (2471 Views)
Background: Alpinia galanga (A. galanga) belongs to the Zingiberaceae family has anti-oxidant effects in animals and humans body and often is used as medicament or part of medicaments in Asian folk medicine for various applications.
Objective: The objective of this study was to investigate the molecular and biochemical influence of alcoholic extract from the rhizomes of A. galangal on the spermatogenesis process in male rat.
Materials and Methods: Forty five Wistar male rats were divided into three groups, control (n=15) and two tested groups (n=30). Alcoholic extract (5%) of plant was given by oral route at doses of 100 and 300 mg/day for 56 days and spermatogenesis parameters, hormone changes and expression level of the cAMP-responsive element modulator (CREM) gene were assessed.
Results: Methanol extract of A. galanga increased serum testosterones level significantly in both treated groups in comparison with control group (p<0.05). Besides, the percentage of sperm viability and motility in both tested groups were significantly increased. Follicle stimulating hormone FSH hormone, morphology and weight were affected in both treated groups. With 300 mg/day an increase in sperm count was observed. Sperm motility was increased in two treated groups whereas testis weight was decreased in treated groups. Real time analysis of treated cells of testis showed increase level of mRNA related to CREM gene involved in spermatogenesis process after 56 days induction.
Conclusion: It is concluded that application of ethanolic extract of A. galanga significantly increased sperm percentage, viability, motility and testosterone hormone. This suggested that this plant may be promising in enhancing sperm healthy parameters.
Objective: The objective of this study was to investigate the molecular and biochemical influence of alcoholic extract from the rhizomes of A. galangal on the spermatogenesis process in male rat.
Materials and Methods: Forty five Wistar male rats were divided into three groups, control (n=15) and two tested groups (n=30). Alcoholic extract (5%) of plant was given by oral route at doses of 100 and 300 mg/day for 56 days and spermatogenesis parameters, hormone changes and expression level of the cAMP-responsive element modulator (CREM) gene were assessed.
Results: Methanol extract of A. galanga increased serum testosterones level significantly in both treated groups in comparison with control group (p<0.05). Besides, the percentage of sperm viability and motility in both tested groups were significantly increased. Follicle stimulating hormone FSH hormone, morphology and weight were affected in both treated groups. With 300 mg/day an increase in sperm count was observed. Sperm motility was increased in two treated groups whereas testis weight was decreased in treated groups. Real time analysis of treated cells of testis showed increase level of mRNA related to CREM gene involved in spermatogenesis process after 56 days induction.
Conclusion: It is concluded that application of ethanolic extract of A. galanga significantly increased sperm percentage, viability, motility and testosterone hormone. This suggested that this plant may be promising in enhancing sperm healthy parameters.
Type of Study: Original Article |
References
1. Damti O, Sarid O, Sheiner E, Zilberstein T, Cwikel J. Stress and distress in infertility among women. Harefuah 2008; 147: 256-260.
2. Blumer CG, Fariello RM, Restelli AE, Spaine DM, Bertolla RP, Cedenho AP. Sperm nuclear DNA fragmentation and mitochondrial activity in men with varicocele. Fertil steril 2008; 90: 1716-1722. [DOI:10.1016/j.fertnstert.2007.09.007]
3. Mosher WD, Pratt WF. Fecundity and infertility in the United States: incidence and trends. Fertil Steril 1991; 56: 192-3.
4. Lu P, Lai S, Liang P, Chen T, Shun SQ. Antioxidation activity and protective effection of ginger oil on DNA damage in vitro. Zhongguo Zhong Yao Za Zhi 2003; 28: 873-875. (In Chinese)
5. Mayachiew P, Devahastin S. Antimicrobial and antioxidant activities of Indian gooseberry and galangal extracts. LWT Food Sci Technol 2008; 41: 1153-1159. [DOI:10.1016/j.lwt.2007.07.019]
6. Ghasemzadeh A, Jaafar E, Rahmat A. Antioxidant Activities, Total Phenolics and Flavonoids Content in Two Varieties of Malaysia Young Ginger (Zingiber officinale Roscoe). Molecules 2010; 15: 4324-4333. [DOI:10.3390/molecules15064324]
7. Chudiwal A, Jain D, Somani R. Alpinia galanga Willd. An overview on phyto-pharmacological properties Indian J Nat Prod Resour 2010; 1: 143-149.
8. Kaushik D, Yadav J, Kaushik P, Sacher D, Rani R. Current pharmacological and phytochemical studies of the plant Alpinia galanga. Zhong Xi Yi Jie He Xue Bao 2011; 9: 1061-1065. [DOI:10.3736/jcim20111004]
9. Khaki A, Fathiazad F, Nouri M, Khaki AA, Ozanci Chelar C, Ghafari-Novin M, et al. The effects of Ginger on spermatogenesis and sperm parameters of rat. Iran J Reprod Med 2009; 7: 7-12.
10. Steger K, Behr R, Kleiner I, Weinbauer GF, Bergmann M. Expression of activator of CREM in the testis (ACT) during normal and impaired spermatogenesis: correlation with CREM expression. Mol Hum Reprod 2004; 10: 129-135. [DOI:10.1093/molehr/gah012]
11. Blocher S, Fink L, Bohle RM, Bergmann M, Steger K. CREM activator and repressor isoform expression in human male germ cells. Int J Androl 2005; 28: 213-215. [DOI:10.1111/j.1365-2605.2005.00532.x]
12. Gonzales GF, Rubio J, Chung A, Gasco M, Villegas L. Effect of alcoholic extract of Lepidium meyenii (Maca) on testicular function in male rats. Asian J Androl 2003; 5: 349-352.
13. Padmalatha RS, Vijayalaxmi K. amoxifen citrate induced sperm shape abnormalities in the in vitro mouse Mutat Res. 492, 1-6.es of selected testis components, emphasizing those related to the Sertoli cell. Am J Anat 2001; 188: 21-30.
14. Padmalatha RS, Vijayalaxmi K. Tamoxifen citrate induced sperm shape abnormalities in the in vitro mouse. Mutat Res 2001; 492: 1-6. [DOI:10.1016/S1383-5718(00)00160-1]
15. Ptaffi MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acid Res 2001; 29: 2002-2007.
16. Estakhr J, Javadian N, Motalleb G, Sanchooli N, Marzban N, Shams Lahijani M. Evaluation of the effects of Salvia hypoleuca on the cAMP-responsive element modulator (CREM) gene expression and spermatogenesis in rat. Middle East Fertil Soc J 2010; 15: 274-277. [DOI:10.1016/j.mefs.2010.08.002]
17. Qureshi S, Shah AH, Ageel AM. Toxicity studies on Alpinia galanga and Curcuma longa. Planta Med 1992; 58: 124-127. [DOI:10.1055/s-2006-961412]
18. Esposito G, Jaiswal BS, Xie F, Krajnc-Franken MA, Robben TJ, Strik AM, et al. Mice deficient for soluble adenylyl cyclase are infertile because of a severe sperm-motility defect. Proc Natl Acad Sci USA 2004; 101: 2993-2998. [DOI:10.1073/pnas.0400050101]
19. Chandiran S, Swamy B, Kumar BP, Narayanan V, Kumar SV, Muralidharan P, et al. Studies on spermatotoxic effect of ethanolic extract of root of 'Caesalpinia digyna (Rottler). Pharmacologyonline 2008; 2: 790-795.
20. Al-Qarawi AA. Stimulatory effect of the aqueous extract of Ruta chalepensis on the sex organs and hormones of male rats. J Appl Res 2005; 5: 206-211.
21. Srividya A, Dhana
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