Volume 12, Issue 4 (5-2014)                   IJRM 2014, 12(4): 249-256 | Back to browse issues page

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Khodayari Naeini Z, Hassani Bafrani H, Nikzad H. Evaluation of ebselen supplementation on cryopreservation medium in human semen. IJRM 2014; 12 (4) :249-256
URL: http://ijrm.ir/article-1-532-en.html
Evaluation of ebselen supplementation on cryopreservation medium in human semen
Zohreh Khodayari Naeini1 , Hassan Hassani Bafrani * 2, Hossein Nikzad1
1- Kashan Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
2- Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran , hasanhasani@yahoo.com
Abstract:   (2675 Views)
Background: An effect of cryopreservation on human sperm is sublethal cryodamage, in which cell viability post-thaw is lost more rapidly at later times than in fresh cells.
Objective: This study examined whether the addition of an antioxidant to cryopreservation medium could improve the post-thaw parameters and evaluation of sperm chromatin quality of cryopreserved human spermatozoa from men with normal semen parameters.
Materials and Methods: Semen samples (n=35) were collected by masturbation and assessed following WHO standards. Individual samples were classified as two portions. One portion (n=10) was for elucidate the concentration of ebselen.Then the samples(n=25) were divided in to 5groups.The first aliquot remained fresh.The second aliquots was mixed with cryopreservation medium.The third aliquots were mixed with cryopreservation medium containing solvent of ebselen.The forth and fifth aliquots were mixed with cryopreservation medium containing 1.25 and 2.5 µm of ebselen.Samples were frozen and thawed samples were assessed for sperm parameters.Three-way ANOVA Multivariate measures were used to assess. According to this assesment the differences are observed in existent groups in post-thaw count, motility index, vitality staining, and morphology and DNA fragmentation.
Results: After freezing the media containing of ebselen, DNA fragmentation is significantly different in comparison with control group. ebselen with 1.25 µm dose was significantly associated with post-thaw DNA fragmentation (p=0.047). Similarly ebselen with 2.5 µm dose was significantly associated with post-thaw DNA fragmentation (p=0.038). But other parameters were not altered.
Conclusion: These results suggest that the addition of ebselen to cryopreservation medium doesnot improve post-thaw parameters and DNA fragmentation of sperm
Keywords: Cryopreservation, Ebselen, DNA fragmentation, Human sperm.
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Type of Study: Original Article |
References
1. Anger JT, Gilbert BR, Goldstein M. Cryopreservation of sperm: indications, methods and results. J Urol 2003;170: 1079-1084. [DOI:10.1097/01.ju.0000084820.98430.b8]
2. Tournaye H, Goossens E, Verheyen G, Frederickx V, De Block G, Devroey P, et al. Preserving the reproductive potential of men and boys with cancer: current concepts and future prospects. Hum Reprod Update 2004; 10: 525-532. [DOI:10.1093/humupd/dmh038]
3. Ball BA, Vo AT, Baumber J. Generation of reactive oxygen species by equine spermatozoa. Am J Veterinar Res 2001; 62: 508-515. [DOI:10.2460/ajvr.2001.62.508]
4. Baumber J, Ball BA, Linfor JJ, Meyers SA. Reactive oxygen species and cryopreservation promote DNA fragmentation in equine spermatozoa. J Androl 2003; 24: 621. [DOI:10.1002/j.1939-4640.2003.tb02714.x]
5. Chatterjee S, Gagnon C. Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Mol Reprod Dev 2001; 59: 451-458. [DOI:10.1002/mrd.1052]
6. Critser J, Huse-Benda A, Aaker D, Arneson B, Ball G. Cryopreservation of human spermatozoa. III. The effect of cryoprotectants on motility. Fertil Steril 1988; 50: 314. [DOI:10.1016/S0015-0282(16)60079-1]
7. Kalthur G, Adiga SK, Upadhya D, Rao S, Kumar P. Effect of cryopreservation on sperm DNA integrity in patients with teratospermia. Fertil Steril 2008; 89: 1723-1727. [DOI:10.1016/j.fertnstert.2007.06.087]
8. Kim JG, Parthasarathy S, editors. Oxidation and the spermatozoa. Georg Thieme Verlag, 1998.
9. Bansal A, Bilaspuri G. Effect of manganese on bovine sperm motility, viability, and lipid peroxidation in vitro. Anim Reprod CBRA 2008; 5: 90-96.
10. Kumar H, Mahmood S. The use of fast acting antioxidants for the reduction of cow placental retention and subsequent endometritis. Indian J Anim Sci 2001; 71: 650-653.
11. Miller J, Brzezinska-Slebodzinska E, Madsen F. Oxidative stress, antioxidants, and animal function. J Dairy Sci 1993; 76: 2812-2823. [DOI:10.3168/jds.S0022-0302(93)77620-1]
12. Askari H, Check J, Peymer N, Bollendorf A. Effect of natural antioxidants tocopherol and ascorbic acids in maintenance of sperm activity during freeze-thaw process. Syst Biol Reprod Med 1994; 33: 11-15. [DOI:10.3109/01485019408987797]
13. Roca J, Rodriguez MJ, Gil MA, Carvajal G, Garcia EM, Cuello C, et al. Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and/or catalase. J Androl 2005; 26: 15.
14. Bhabak KP, Mugesh G. Synthesis, characterization, and antioxidant activity of some ebselen analogues. Chemistry-A Eur J 2007; 13: 4594-4601. [DOI:10.1002/chem.200601584]
15. Parnham M, Sies H. Ebselen: prospective therapy for cerebral ischaemia. Expert Opin Invest Drugs 2000; 9: 607-619. [DOI:10.1517/13543784.9.3.607]
16. Saito I, Asano T, Sano K, Takakura K, Abe H, Yoshimoto T, et al. Neuroprotective effect of an antioxidant, ebselen, in patients with delayed neurological deficits after aneurysmal subarachnoid hemorrhage. Neurosurgery 1998; 42: 269. [DOI:10.1097/00006123-199802000-00038]
17. Imai H, Graham DI, Masayasu H, Macrae IM. Antioxidant ebselen reduces oxidative damage in focal cerebral ischemia. Free Rad Biol Med 2003; 34: 56-63. [DOI:10.1016/S0891-5849(02)01180-2]
18. Kil J, Pierce C, Tran H, Gu R, Lynch ED. Ebselen treatment reduces noise induced hearing loss via the mimicry and induction of glutathione peroxidase. Hearing Res 2007; 226: 44-51. [DOI:10.1016/j.heares.2006.08.006]
19. Maiorino M, Roveri A, Coassin M, Ursini F. Kinetic mechanism and substrate specificity of glutathione peroxidase activity of ebselen (PZ51). Biochem Pharmacol 1988; 37: 2267-2271. [DOI:10.1016/0006-2952(88)90591-6]
20. Organization WH. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge university press; 1999.
21. Sherman J. Cryopreservation of human semen. CRC Handbook of the Laboratory Diagnosis and Treatment of Infertility. Edited by BA Keel, Webster BW. Boston, CRC Press Inc; 1990: 229.
22. Fernandez JL, Muriel L, Rivero MT, Goyanes V, Vazquez R, Alvarez JG. The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation. J Androl 2003; 24: 59.
23. Medeiros C, Forell F, Oliveira A, Rodrigues J. Current status of sperm cryopreservation: why isn't it better? Theriogenology 2002; 57: 327-344. [DOI:10.1016/S0093-691X(01)00674-4]
24. Bilodeau JF, Chatterjee S, Sirard MA, Gagnon C. Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol Reprod Dev 2000; 55: 282-288. https://doi.org/10.1002/(SICI)1098-2795(200003)55:3<282::AID-MRD6>3.0.CO;2-7 [DOI:10.1002/(SICI)1098-2795(200003)55:33.0.CO;2-7]
25. Gadea J, Sellés E, Marco MA, Coy P, Matás C, Romar R, et al. Decrease in glutathione content in boar sperm after cryopreservation: Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology 2004; 62: 690-701. [DOI:10.1016/j.theriogenology.2003.11.013]
26. Chi H, Kim J, Ryu C, Lee J, Park J, Chung D, et al. Protective effect of antioxidant supplementation in sperm-preparation medium against oxidative stress in human spermatozoa. Hum Reprod 2008; 23: 1023-1028. [DOI:10.1093/humrep/den060]
27. Thuwanut P, Chatdarong K, Techakumphu M, Axnér E. The effect of antioxidants on motility, viability, acrosome integrity and DNA integrity of frozen-thawed epididymal cat spermatozoa. Theriogenology 2008; 70: 233-240. [DOI:10.1016/j.theriogenology.2008.04.005]
28. Rossi T, Mazzilli F, Delfino M, Dondero F. Improved human sperm recovery using superoxide dismutase and catalase supplementation in semen cryopreservation procedure. Cell and Tissue Banking 2001; 2: 9-13. [DOI:10.1023/A:1011592621487]
29. Maiorino M, Roveri A, Coassin M, Ursini F. Kinetic mechanism and substrate specificity of glutathione peroxidase activity of ebselen (PZ51). Biochem Pharmacol 1988; 37: 2267-2271. [DOI:10.1016/0006-2952(88)90591-6]
30. Li Z, Lin Q, Liu R, Xiao W, Liu W. Protective effects of ascorbate and catalase on human spermatozoa during cryopreservation. J Androl 2010; 31: 437. [DOI:10.2164/jandrol.109.007849]
31. Donnelly ET, Steele EK, McClure N, Lewis SEM. Assessment of DNA integrity and morphology of ejaculated spermatozoa from fertile and infertile men before and after cryopreservation. Hum Reprod 2001; 16: 1191-1199. [DOI:10.1093/humrep/16.6.1191]
32. Donnelly ET, McClure N, Lewis SEM. Antioxidant supplementation in vitro does not improve human sperm motility. Fertil Steril 1999; 72: 484-495. [DOI:10.1016/S0015-0282(99)00267-8]
33. Branco CS, Garcez ME, Pasqualotto FF, Erdtman B, Salvador M. Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen. Cryobiology 2010; 60: 235-237. [DOI:10.1016/j.cryobiol.2009.10.012]
34. Taylor K, Roberts P, Sanders K, Burton P. Effect of antioxidant supplementation of cryopreservation medium on post-thaw integrity of human spermatozoa. Reprod Biomed Online 2009; 18: 184-189. [DOI:10.1016/S1472-6483(10)60254-4]
35. Garcez ME, dos Santos Branco C, Lara LV, Pasqualotto FF, Salvador M. Effects of resveratrol supplementation on cryopreservation medium of human semen. Fertil Steril 2010; 94: 2118-2121. [DOI:10.1016/j.fertnstert.2010.01.058]
36. Iwanier K, Zachara BA. Selenium supplementation enhances the element concentration in blood and seminal fluid but does not change the spermatozoal quality characteristics in subfertile men. J Androl 1995; 16: 441-447.
37. Azad GK, Balkrishna SJ, Narayanan S, Kumar S, Tomar RS. Multifunctional ebselen drug function through the activation of DNA damage response and alterations in nuclear proteins. Biochem Pharmacol 2011.
38. Ford W, Whittington K. Antioxidant treatment for male subfertility: a promise that remains unfulfilled. Hum Reprod 1998; 13: 1416-1419. [DOI:10.1093/oxfordjournals.humrep.a019707]
39. Lenzi A, Gandini L, Picardo M. A rationale for glutathione therapy. Hum Reprod 1998; 13: 1419-1422. [DOI:10.1093/oxfordjournals.humrep.a019708]
40. Tarin J, Brines J, Cano A. Antioxidants may protect against infertility. Hum Reprod 1998; 13: 1415-1416. [DOI:10.1093/humrep/13.6.1415]
41. Martin-Du Pan R, Sakkas D. Is antioxidant therapy a promising strategy to improve human reproduction? Are anti-oxidants useful in the treatment of male infertility? Hum Reprod 1998; 13: 2984-2985. [DOI:10.1093/humrep/13.11.2984]
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