Volume 12, Issue 5 (6-2014)                   IJRM 2014, 12(5): 327-0 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Amirjannati N, Yaghmaei F, Akhondi M M, Nasiri M, Heidari-Vala H, Sehhat Z. Molecular and serologic diagnostic approaches; the prevalence of herpes simplex in idiopathic men infertile. IJRM 2014; 12 (5) :327-0
URL: http://ijrm.ir/article-1-540-en.html
Molecular and serologic diagnostic approaches; the prevalence of herpes simplex in idiopathic men infertile
Nasser Amirjannati1 , Farhad Yaghmaei * 2, Mohammad Mehdi Akhondi1 , Mahboubeh Nasiri3 , Hamed Heidari-Vala4 , Zahra Sehhat1
1- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
2- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , yaghmaie@avicenna.ac.ir
3- Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
4- Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Abstract:   (2440 Views)
Background: Human pathogens that can cause infertility may also affect sperm count and quality. Viral infections can be considered as direct and/or indirect cause of male factor infertility.
Objective: Our goal was to investigate the prevalence of herpes simplex virus in the semen of infertile men attending the Avicenna Infertility Clinic, and to compare it with the herpes virus serology results.
Materials and Methods: This cross sectional study was conducted during 2009-2010. Infertile men participating without any clinical signs of infection with herpes simplex virus, and no obvious cause for their infertility were included. Semen and blood samples were used for Polymerase Chain Reaction (PCR) and serologic testing for these people. Two samples were collected: one ml semen sample to verify the existence of genital herpes simplex virus in infertile men, and blood samples of 217 individuals tested for antibodies to herpes simplex virus. Data were analyzed by SPSS 16.
Results: According to the PCR results of semen samples the prevalence of herpes simplex in semen was 12% and serologic test showed 3.2% prevalence within blood. Nine to 10% of IgM negative were PCR positive and only 2-3% of IgM positive were PCR positive. Between herpes serologic studies with positive controls and negative controls by using both tests, there was a significant positive relationship (r=0.718 and p<0.001). The relationship between semen PCR test results and serological survey of herpes patients with a negative control in both Pearson and Spearman tests was positive and significant (r=0.229 and p=0.001). Correlation between the PCR results of semen samples with two positive control subjects and a positive IgM test was statistically confirmed (r=0.235 and p<0.001).
Conclusion: We recommend that if there is suspicion to herpes simplex as a microorganism that theoretically could impact semen parameters and cause infertility it is prudent to use PCR technique on semen sample rather than ELISA on serum.
Keywords: Herpes virus, Infertility, IgM, Polymerase Chain Reaction, Semen, Serologic, Sexually transmitted diseases (STD).
Full-Text [PDF 186 kb]   (714 Downloads) |   |   Full-Text (HTML)  (402 Views)  
Type of Study: Original Article |
References
1. Mohammad K, Ardalan A. An Overview of the Epidemiology of Primary Infertility in Iran. J Reprod Infertil 2009; 10: 213-216.
2. Kapranos N, Petrakou E, Anastasiadou C, Kotronias D. Detection of herpes simplex virus, cytomegalovirus, and Epstein-Barr virus in the semen of men attending an infertility clinic. Fertil Steril 2003; 79: 1566-1570. [DOI:10.1016/S0015-0282(03)00370-4]
3. Mortimer D. The future of male infertility management and assisted reproduction technology. Hum Reprod 2000; 15 (Suppl): 98-110. [DOI:10.1093/humrep/15.suppl_5.98]
4. Neofytou E, Sourvinos G, Asmarianaki M, Spandidos DA, Makrigiannakis A. Prevalence of human herpes virus types 1-7 in the semen of men attending an infertility clinic and correlation with semen parameters. Fertil Steril 2009; 91: 2487-2494. [DOI:10.1016/j.fertnstert.2008.03.074]
5. Leruez-Ville M, Galimand J, Ghosn J, Briat A, Delaugerre C, Chaix ML. Male genital tract infection: the point of view of the virologist. Gynecol Obstet Fertil 2005; 33: 684-690. [DOI:10.1016/j.gyobfe.2005.07.011]
6. Vayena E, Rowe P, Griffin P. Current practices and controversies in assisted reproduction: Report of a meeting on "Medical, Ethical and Social Aspects of Assisted Reproduction" held at WHO. 1st Ed. Geneva, World Health Organization; 2002.
7. Radchenko OR. Gender approach to studying reproductive aims in young people. Gig Sanit 2011; 4: 70-73.
8. Zong HT, Peng XX, Yang CC, Zhang Y. Impacts of different transurethral prostatic resection procedures on male sexual function: meta-analysis of randomized controlled trials. Zhonghua Nan Ke Xue 2011; 17: 1014-1018.
9. Hafez E, Hafez S. Atlas of Clinical Andrology. 1st Ed. London, Taylor & Francis Group; 2005. [DOI:10.1201/b14619]
10. Lédée N, Rahmati M. Human infertility: management of the first consultation. Rev Prat 2011; 61: 983-987.
11. Eke AC, Okafor CI, Ezebialu IU. Male infertility management in a Nigerian tertiary hospital. Int J Gynaecol Obstet 2011; 114: 85-86. [DOI:10.1016/j.ijgo.2011.01.023]
12. Bayasgalan G, Naranbat D, Radnaabazar J, Lhagvasuren T, Rowe PJ. Male infertility: risk factors in Mongolian men. Asian J Androl 2004; 6: 305-311.
13. Dejucq-Rainsford N, Jegou B. Viruses in semen and male genital tissues-consequences for the reproductive system and therapeutic perspectives. Curr Pharm Des 2004; 10: 557-575 . [DOI:10.2174/1381612043453225]
14. WHO. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction, 4th Ed. London, Cambridge University Press; 1999.
15. Pallier C, Tebourbi L, Chopineau-Proust S, Schoevaert D, Nordmann P, Testart J, et al. Herpesvirus, cytomegalovirus, human sperm and assisted fertilization. Hum Reprod 2002; 17: 1281-1287. [DOI:10.1093/humrep/17.5.1281]
16. Marconi M, Pilatz A, Wagenlehner F, Diemer T, Weidner W. Impact of infection on the secretory capacity of the male accessory glands. Int Braz J Urol 2009; 35: 299-308. [DOI:10.1590/S1677-55382009000300006]
17. Dejucq N, Jegou B. Viruses in the mammalian male genital tract and their effects on the reproductive system. Microbiol Mol Biol Rev 2001; 65: 208-231. [DOI:10.1128/MMBR.65.2.208-231.2001]
18. Foresta C, Pizzol D, Bertoldo A, Menegazzo M, Barzon L, Garolla A. Semen washing rocedures do not eliminate human papilloma virus sperm infection in infertile patients. Fertil Steril 2011; 96: 1077-1082. [DOI:10.1016/j.fertnstert.2011.04.009]
19. Dulioust E, Du AL, Costagliola D, Guibert J, Kunstmann JM, Heard I, et al. Semen alterations in HIV-1 infected men. Hum Reprod 2002; 17: 2112-2118. [DOI:10.1093/humrep/17.8.2112]
20. Dias CF, Nunes CC, Freitas IO, Lamego IS, Oliveira IM, Gilli S, et al. High prevalence and association of HIV-1 non-B subtype with specific sexual transmission risk among antiretroviral naïve patients in Porto Alegre, RS, Brazil. Rev Inst Med Trop Sao Paulo 2009; 51: 191-196. [DOI:10.1590/S0036-46652009000400003]
21. Bezold G, Schuster-Grusser A, Lange M, Gall H, Wolff H, Peter RU. Prevalence of human herpesvirus types 1-8 in the semen of infertility patients and correlation with semen parameters. Fertil Steril 2001; 76: 416-418. [DOI:10.1016/S0015-0282(01)01920-3]
22. Tremellen K, Oxidative stress and male infertility-a clinical perspective. Hum Reprod Update 2008; 14: 243-258. [DOI:10.1093/humupd/dmn004]
23. Asgari S, Chamani-Tabriz L, Asadi S, Fatemi F, Zeraati H, Akhondi MM, et al. HSV-2 Seroepidemiology and Risk Factors among Iranian Women: A Time to New Thinking. Iran Red Crescent Med J 2011; 13: 818-823.
24. Salehi-vaziri M, Monavari SH, Khalili M, Shamsi-Shahrabadi M, Keyvani H, Mollaei H, et al. Detection of HSV-1 DNA in the Semen of Infertile Men and Evaluation of its Correlation with Semen Parameters in Iran. Iran J Virol 2010; 4: 1-6. [DOI:10.21859/isv.4.2.1]
25. Kailash U, Hedau S, Gopalkrishna V, Katiyar S, Das BC. A simple 'paper smear' method for dry collection, transport and storage of cervical cytological specimens for rapid screening of HPV infection by PCR. J Med Microbiol 2002; 51: 606-683. [DOI:10.1099/0022-1317-51-7-606]
26. Wysocka J, Herr W. The herpes simplex virus VP16-induced complex: the makings of a regulatory switch. Trends Biochem Sci 2003; 28: 294-304. [DOI:10.1016/S0968-0004(03)00088-4]
27. Smith JS, Robinson NJ. Age-specific prevalence of infection with herpes simplex virus types 2 and 1: a global review. J Infect Dis 2002; 186 (Suppl.): 3-28. [DOI:10.1086/343739]
28. Brooks GF. Medical Microbiology. 25th Ed. London, Appleton & Lange; 2010.
29. Collier LH, Oxford JS. Human Virology. 2nd Ed. Oxford, Oxford University Press; 1999.
30. Zaravinos A, Bizakis J, Spandidos DA. Prevalence of human papilloma virus and human herpes virus types 1-7 in human nasal polyposis. J Med Virol 2009; 81: 1613-1619. [DOI:10.1002/jmv.21534]
31. Alberts CJ, Schim van der Loeff MF, Papenfuss MR, da Silva RJ, Villa LL, Lazcano-Ponce E, et al. Association of Chlamydia trachomatis Infection and Herpes Simplex Virus Type 2 Serostatus With Genital Human Papillomavirus Infection in Men: The HPV in Men Study. Sex Transm Dis 2013; 40: 508-515. [DOI:10.1097/OLQ.0b013e318289c186]
32. Aynaud O, Poveda JD, Huynh B, Guillemotonia A, Barrasso R. Frequency of herpes simplex virus, cytomegalovirus and human papillomavirus DNA in semen. Int J STD AIDS 2002; 13: 547-550. [DOI:10.1258/095646202760159666]
33. Wald A, Matson P, Ryncarz A, Corey L. Detection of herpes simplex virus DNA in semen of men with genital HSV-2 infection. Sex Transm Dis 1999; 26: 1-3. [DOI:10.1097/00007435-199901000-00001]
34. el Borai N, Inoue M, Lefèvre C, Naumova EN, Sato B, Yamamura M. Detection of herpes simplex DNA in semen and menstrual blood of individuals attending an infertility clinic. J Obstet Gynaecol Res 1997; 23: 17-24. [DOI:10.1111/j.1447-0756.1997.tb00799.x]
35. Foresta C, Patassini C, Bertoldo A, Menegazzo M, Francavilla F, Barzon L, et al. Mechanism of human papillomavirus binding to human spermatozoa and fertilizing ability of infected spermatozoa. PLoS One 2011; 6: e15036. [DOI:10.1371/journal.pone.0015036]
36. Todd J, Riedner G, Maboko L, Hoelscher M, Weiss HA, Lyamuya E, et al. Effect of genital herpes on cervicovaginal HIV shedding in women co-infected with HIV AND HSV-2 in Tanzania. PLoS One 2013; 8: e59037. [DOI:10.1371/journal.pone.0059037]
37. Bezold G, Politch JA, Kiviat NB, Kuypers JM, Wolff H, Anderson DJ. Prevalence of sexually transmissible pathogens in semen from asymptomatic male infertility patients with and without leukocytospermia. Fertil Steril 2007; 87: 1087-1097. [DOI:10.1016/j.fertnstert.2006.08.109]
38. Smith JS, Rosinska M, Trzcinska A, Pimenta JM, Litwinska B, Siennicka J. Type specific seroprevalence of HSV-1 and HSV-2 in four geographical regions of Poland. Sex Transm Infect 2006; 82: 159-163. [DOI:10.1136/sti.2005.015446]
39. Cunningham AL, Taylor R, Taylor J, Marks C, Shaw J, Mindel A. Prevalence of infection with herpes simplex virus types 1 and 2 in Australia: a nationwide population based survey. Sex Transm Infect 2006; 82: 164-168. [DOI:10.1136/sti.2005.016899]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb