Volume 12, Issue 5 (6-2014)                   IJRM 2014, 12(5): 301-0 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Moein M R, Vahidi S, Ghasemzadeh J, Tabibnejad N. Comparison of reactive oxygen species in neat and washed semen of infertile men. IJRM 2014; 12 (5) :301-0
URL: http://ijrm.ir/article-1-542-en.html
Comparison of reactive oxygen species in neat and washed semen of infertile men
Mohammad Reza Moein * 1, Serajedin Vahidi2 , Jalal Ghasemzadeh2 , Nasim Tabibnejad2
1- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , moein1339@yahoo.com
2- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract:   (2270 Views)
Background: Male are involved in near 50% of cases of infertility and reactive oxygen species (ROS) playing an important role in decreasing fertility potential. Accurate measurement of ROS seems to be important in evaluation of infertile male patients.
Objective: To compare ROS measurement in neat and washed semen samples of infertile men and define the best method for evaluation of ROS in these patients.
Materials and Methods: We measured the level of ROS in semen samples of thirty five non-azoospermic men with infertility. The semen samples were divided into two parts and the semen parameters and ROS levels in neat and washed samples were evaluated. We also evaluated the presence of pyospermia using peroxidase test.
Results: The differences regarding sperm count and quick motility were significant in neat and washed semen samples. The mean ROS level was significantly higher in neat samples compared with washed spermatozoa (7.50 RLU vs. 1.20 RLU respectively). Difference in ROS levels was more significant in patients with pyospermia compared to whom with no pyospermia (378.67 RLU vs. 9.48 RLU respectively).
Conclusion: Our study confirmed that neat or unprocessed samples are better index of normal oxidative status of semen samples. Because we do not artificially add or remove factors that may play an important role in oxidative equilibrium status.
Keywords: ROS, Neat sperm, Washed sperm, Pyospermia
Full-Text [PDF 106 kb]   (706 Downloads) |   |   Full-Text (HTML)  (408 Views)  
Type of Study: Original Article |
References
1. Sharlip ID, Jarow JP, Belker AM, Lipshultz LI, Sigman M, Thomas AJ, et al. Best practice policies for male infertility. Fertil Steril 2002; 77: 873-882. [DOI:10.1016/S0015-0282(02)03105-9]
2. Alkan I, Simsek F, Haklar G, Kervancioglu E, Ozveri H, Yalcin S, et al. Reactive oxygen species production by spermatozoa of patients with idiopathic infertility: relationship to seminal plasma antioxidants. J Urol 1997; 157: 140-143. [DOI:10.1016/S0022-5347(01)65307-2]
3. Agarwal A, Said TM. Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach. BJU Int 2005; 95: 503-507. [DOI:10.1111/j.1464-410X.2005.05328.x]
4. Benedetti S, Tagliamonte MC, Catalani S, Primiterra M, Canestrari F, De Stefani S, et al. Differences in blood and semen oxidative status in fertile and infertile men, and their relationship with sperm quality. Reprod Biomed Online 2012; 25: 300- 306. [DOI:10.1016/j.rbmo.2012.05.011]
5. De Lamirande E, Gagnon C. Human sperm hyperactivation and capacitation as part of an oxidative process. Free Radic Biol Med 1993; 14: 157-166. [DOI:10.1016/0891-5849(93)90006-G]
6. Agarwal A, Makker K, Sharma R. Clinical relevance of oxidative stress in male factor infertility: an update. Am J Reprod Immunol 2008; 59: 2-11. [DOI:10.1111/j.1600-0897.2007.00559.x]
7. Aitken RJ, McLaughlin EA. Molecular mechanisms of sperm capacitation: progesterone-induced secondary calcium oscillation reflect the attainment of a capacitated state. Soc Reprod Fertil Suppl 2007; 63: 273-293.
8. De Lamirande E, O'Flaherry C. Sperm activation: role of reactive oxygen species and kinases. Biochim Biophys Acta 2008; 1784: 106-115. [DOI:10.1016/j.bbapap.2007.08.024]
9. Roveri A, Ursini F, Flohe L, Maiorino M. PHGPx and spermatogenesis. Biofactors 2001; 14: 213-222. [DOI:10.1002/biof.5520140127]
10. Aitken RJ, Irvines DS, Wu FC. Prospective analysis of sperm-oocyte fusion and reactive oxygen species generation as criteria for the diagnosis of infertility. Am J Obstet Gynecol 1991; 164: 542-551. [DOI:10.1016/S0002-9378(11)80017-7]
11. Zorn B, Vidmar J, Meden-Vrtovec H. Seminal reactive oxygen species as predictors of fertilization, embryo quality and pregnancy rates after conventional in vitro fertilization and intracytoplasmic sperm injection. Int J Androl 2003; 26: 279-285. [DOI:10.1046/j.1365-2605.2003.00424.x]
12. Padron OF, Brackett NL, Sharma RK, Lynne CM, Thomas AJ Jr, Agarwal A. Seminal reactive oxygen species and sperm motility and morphology in men with spinal cord injury. Fertil Steril 1997; 67: 1115-1120. [DOI:10.1016/S0015-0282(97)81448-3]
13. Agarwal A, Allamaneni SS, Said TM. Chemiluminescence technique for measuring reactive oxygen species. Reprod Biomed Online 2004; 9: 466-468. [DOI:10.1016/S1472-6483(10)61284-9]
14. Venkatesh S, Shamsi MB, Dudeja S, Kumar R, Dada R. Reactive oxygen species measurement in neat and washed semen: comparative analysis and its significance in male infertility assessment. Arch Gynecol Obstet 2011; 283: 121-126. [DOI:10.1007/s00404-010-1645-4]
15. Agarwal A, Ikemoto I, Loughlin KR. Effect of sperm washing on levels of reactive oxygen species in semen. Arch Androl 1994; 33: 157-162. [DOI:10.3109/01485019408987819]
16. Allamaneni SS, Agarwal A, Nallella KP, Sharma RK, Thomas AJ Jr, Sikka SC Characterization of oxidative stress status by evaluation of reactive oxygen species levels in whole semen and isolated spermatozoa. Fertil Steril 2005; 83: 800-803. [DOI:10.1016/j.fertnstert.2004.05.106]
17. World Health Organization. Laboratory manual for the examination of human semen and sperm-cervical mucus interaction, 4th Ed. Cambridge University Press, New York; 1999.
18. Shekarriz M, Sharma RK, Thomas AJ Jr, Agarwal A. Positive myeloperoxidase staining (Endtz test) as an indicator of excessive reactive oxygen species in semen. J Assist Reprod Genet 1995; 12: 70-74. [DOI:10.1007/BF02211372]
19. Endtz AW. A rapid staining method for differentiating granulocytes from "germinal cells" in Papanicolaou-stained semen. Acta Cytol 1974; 18: 2-7.
20. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003; 79: 829-843. [DOI:10.1016/S0015-0282(02)04948-8]
21. Henkel R, Kierspel E, Stalf T, Mehnert C, Menkveld R, Tinneberg HR, et al. Effect of reactive oxygen species produced by spermatozoa and leukocytes on sperm functions in non-leukocytospermic patients. Fertil Steril 2005; 83: 635-642. [DOI:10.1016/j.fertnstert.2004.11.022]
22. Moustafa MH, Sharma RK, Thornton J, Mascha E, Abdel-Hafez MA, Thomas AJ Jr, et al. Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Hum Reprod 2004; 19: 129-138. [DOI:10.1093/humrep/deh024]
23. Saleh RA, Agarwal A, Kandirali E, Sharma RK, Thomas AJ, Nada EA, et al. Leukocytospermia is associated with increased reactive oxygen species production by human spermatozoa. Fertil Steril 2002; 78: 1215-1224. [DOI:10.1016/S0015-0282(02)04237-1]
24. Saleh RA, Agarwal A, Sharma RK, Nelson DR, Thomas AJ. Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study. Fertil Steril 2002; 78: 491-499. [DOI:10.1016/S0015-0282(02)03294-6]
25. Cocuzza M, Atayde KS, Agarwal A, Pagani R, Sikka SC, Lucon AM, et al. Impact of clinical varicocele and testis size on seminal reactive oxygen species levels in a fertile population: a prospective controlled study. Fertil Steril 2008; 90: 1103-1108. [DOI:10.1016/j.fertnstert.2007.07.1377]
26. Desai N, Sharma RK, Makler K, Sabanegh E, Agarwal A. Physiologic and pathologic levels of reactive oxygen species in neat semen of infertile men. Fertil Steril 2009; 92: 1626-1631. [DOI:10.1016/j.fertnstert.2008.08.109]
27. Iwasaki A, Gagnon C. Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril 1992; 57: 409-416. [DOI:10.1016/S0015-0282(16)54855-9]
28. Pasqualotto FF, Shrma RK, Kobayashi H, Nelson DR, Thomas AJ, Agarwal A. Oxidative stress in normospermic men undergoing infertility evaluation. J Androl 2001; 22: 316-322.
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb