Volume 12, Issue 6 (8-2014)                   IJRM 2014, 12(6): 389-0 | Back to browse issues page

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Ghasemi N, Amjadi F, Salehi E, Shakeri M, Aflatoonian A, Aflatoonian R. Expression of Toll-like receptors 7-10 in human fallopian tubes. IJRM 2014; 12 (6) :389-0
URL: http://ijrm.ir/article-1-549-en.html
Expression of Toll-like receptors 7-10 in human fallopian tubes
Nasrin Ghasemi1 , Fatemehsadat Amjadi2 , Ensieh Salehi2 , Mojgan Shakeri3 , Abbas Aflatoonian1 , Reza Aflatoonian * 4
1- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Department of Anatomy, Tehran University of Medical Sciences, Tehran, Iran
3- Tehran Islamic Azad University of Medical Sciences, Tehran, Iran
4- Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , R.Aflatoonian@gmail.com
Abstract:   (2408 Views)
Background: The human female reproductive tract (FRT) is constantly deal with the invading pathogens. Recognition of these pathogens is attributed to the family of Toll like receptors (TLR) as a major part of the innate immune system. We and others have previously revealed that TLRs1-6 express in the female reproductive tract. However, more studies should be done to detect TLRs 7-10 in the female reproductive tract, especially in the fallopian tubes.
Objective: To examine the expression of TLRs7-10 in human fallopian tube tissue.
Materials and Methods: Using immunostaining techniques, distribution of TLR7-10 was studied in surgical sections from the uterine tubes, obtained from patients undergoing tubal ligation and hysterectomy for benign gynecological conditions. RT-PCR was used to show the existence of TLR7-10 genes in fallopian tube tissue.
Results: TLR7-10 proteins were detected in the fallopian tube epithelium, although the intensity of staining was not equal in cases. TLR7-10 genes were expressed in human fallopian tube tissue.
Conclusion: This study indicates that TLR7-10 is expressed in fallopian tubes tissues, and may play an important role in microbial recognition, and in host defense against ascending infection.
Keywords: Fallopian tube, Innate immunity, Toll like receptors (TLRs), Reproductive medicine.
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Type of Study: Original Article |
References
1. Wira CR, Patel MV, Ghosh M, Mukura L, Fahey JV. Innate immunity in the human female reproductive tract: endocrine regulation of endogenous antimicrobial protection against HIV and other sexually transmitted infections. Am J Reprod Immunol 2011; 65: 196-211. [DOI:10.1111/j.1600-0897.2011.00970.x]
2. Witkin SS. Immunological aspects of genital chlamydia infections. Best Pract Res Clin Obstet Gynaecol 2002; 16: 865-874. [DOI:10.1053/beog.2002.0326]
3. McCormack WM. Pelvic inflammatory disease. N Engl J Med 1994; 330: 115-119. [DOI:10.1056/NEJM199401133300207]
4. Shaw JL, Wills GS, Lee KF, Horner PJ, McClure MO, Abrahams VM, et al. Chlamydia trachomatis infection increases fallopian tube PROKR2 via TLR2 and NFkappaB activation resulting in a microenvironment predisposed to ectopic pregnancy. Am J Pathol 2011; 178: 253-260. [DOI:10.1016/j.ajpath.2010.11.019]
5. Wira CR, Fahey JV, Sentman CL, Pioli PA, Shen L. Innate and adaptive immunity in female genital tract: cellular responses and interactions. Immunol Rev 2005; 206: 306-335. [DOI:10.1111/j.0105-2896.2005.00287.x]
6. Janeway CA Jr, Medzhitov R. Innate immune recognition. Ann Rev Immunol 2002; 20: 197-216. [DOI:10.1146/annurev.immunol.20.083001.084359]
7. Schaefer TM, Fahey JV, Wright JA, Wira CR. Innate immunity in the human female reproductive tract: antiviral response of uterine epithelial cells to the TLR3 agonist poly (I:C). J Immunol 2005; 174: 992- 1002. [DOI:10.4049/jimmunol.174.2.992]
8. Fazeli A, Bruce C, Anumba DO. Characterization of Toll-like receptors in the female reproductive tract in humans. Hum Reprod 2005; 20: 1372-1378. [DOI:10.1093/humrep/deh775]
9. Kumar H, Kawai T, Akira S. Pathogen recognition by the innate immune system. Int Rev Immunol 2011; 30: 16-34. [DOI:10.3109/08830185.2010.529976]
10. Thompson MR, Kaminski JJ, Kurt-Jones EA, Fitzgerald KA. Pattern recognition receptors and the innate immune response to viral infection. Viruses 2011; 3: 920-940. [DOI:10.3390/v3060920]
11. Akira S, Uematsu S, Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124: 783-801. [DOI:10.1016/j.cell.2006.02.015]
12. Takeda K, Kaisho T, Akira S. Toll-like receptors. Ann Rev Immunol 2003; 21: 335-376. [DOI:10.1146/annurev.immunol.21.120601.141126]
13. Nasu K, Narahara H. Pattern recognition via the toll-like receptor system in the human female genital tract. Mediators Inflamm2010; 2010: 976024.
14. Flacher V, Bouschbacher M, Verronese E, Massacrier C, Sisirak V, Berthier-Vergnes O, et al. Human Langerhans Cells Express a Specific TLR Profile and Differentially Respond to Viruses and Gram-Positive Bacteria. J Immunol 2006; 177: 7959-7967. [DOI:10.4049/jimmunol.177.11.7959]
15. Hasan U, Chaffois C, Gaillard C, Saulnier V, Merck E, Tancredi S, et al. Human TLR10 is a functional receptor, expressed by B cells and plasmacytoid dendritic cells, which activates gene transcription through MyD88. J Immunol 2005; 174: 2942-2950. [DOI:10.4049/jimmunol.174.5.2942]
16. Chuang T, Ulevitch RJ. Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells. Biochim Biophys Acta 2001; 1518: 157-161. [DOI:10.1016/S0167-4781(00)00289-X]
17. Aflatoonian R, Tuckerman E, Elliott SL, Bruce C, Aflatoonian A, Li TC, et al. Menstrual cycle-dependent changes of Toll-like receptors in endometrium. Hum Reprod 2007; 22: 586-593. [DOI:10.1093/humrep/del388]
18. Andersen JM, Al-Khairy D, Ingalls RR. Innate immunity at the mucosal surface: role of toll-like receptor 3 and toll-like receptor 9 in cervical epithelial cell responses to microbial pathogens. Biol Reprod 2006; 74: 824-831. [DOI:10.1095/biolreprod.105.048629]
19. Fichorova RN, Cronin AO, Lien E, Anderson DJ, Ingalls RR. Response to Neisseria gonorrhoeae by cervicovaginal epithelial cells occurs in the absence of toll-like receptor 4-mediated signaling. J Immunol 2002; 168: 2424-2432. [DOI:10.4049/jimmunol.168.5.2424]
20. Hirata T, Osuga Y, Hirota Y, Koga K, Yoshino O, Harada M, et al. Evidence for the presence of toll-like receptor 4 system in the human endometrium. J Clin Endocrinol Metabol 2005; 90: 548-556. [DOI:10.1210/jc.2004-0241]
21. Pioli PA, Amiel E, Schaefer TM, Connolly JE, Wira CR, Guyre PM. Differential expression of Toll-like receptors 2 and 4 in tissues of the human female reproductive tract. Infect Immun 2004; 72: 5799-5806. [DOI:10.1128/IAI.72.10.5799-5806.2004]
22. Pivarcsi A, Nagy I, Koreck A, Kis K, Kenderessy-Szabo A, Szell M, et al. Microbial compounds induce the expression of pro-inflammatory cytokines, chemokines and human beta-defensin-2 in vaginal epithelial cells. Microbes and infection Institut Pasteur. 2005; 7: 1117-1127. [DOI:10.1016/j.micinf.2005.03.016]
23. Saeidi S, Shapouri F, Amirchaghmaghi E, Hoseinifar H, Sabbaghian M, Sadighi Gilani MA, et al. Sperm protection in the male reproductive tract by Toll-like receptors. Andrologia 2013: doi: 10.1111/and.12149. [DOI:10.1111/and.12149]
24. Saeidi S. Shapouri F, Hoseinifar H, Sabbaghian M, Pacey A, Aflatoonian A. Sperm protection by TLRs in the male reproductive tract. Hum Reprod 2012; 27:121.
25. Taghavi SA AM, Mehdizadeh M, Joghataie MT, Aflatoonian R. TLRs expression in follicular cells of poor ovarian responder. J Reprod Immunol 2012; 94: 67-68. [DOI:10.1016/j.jri.2012.03.362]
26. Mohebbi AJA, Nasri S, Lakpour MR, Ramazanali F, Moini A, Aflatoonian R. Expression of toll-like receptors 2, 3 in the endometriosis. Hum Reprod 2012; 27:25.
27. Taghavi SA, Ashrafi M, Karimian L, Mehdizadeh M, Joghataie MT, Aflatoonian R. TLRs expression in follicular cells of infertile PCOS. Hum Reprod 2012; 27:25.
28. Quayle AJ. The innate and early immune response to pathogen challenge in the female genital tract and the pivotal role of epithelial cells. J Reprod Immunol 2002; 57: 61-79. [DOI:10.1016/S0165-0378(02)00019-0]
29. den Hartog JE, Morre SA, Land JA. Chlamydia trachomatis-associated tubal factor subfertility: Immunogenetic aspects and serological screening. Hum Reprod Update 2006; 12: 719-730. [DOI:10.1093/humupd/dml030]
30. Hossain A, Arif M, Ramia S, Bakir TF. Chlamydia trachomatis as a cause of abortion. J Hygiene Epidemiol Microbiol Immunol 1990; 34: 53-55.
31. Jain A, Nag VL, Goel MM, Chandrawati, Chaturvedi UC. Adverse foetal outcome in specific IgM positive Chlamydia trachomatis infection in pregnancy. Indian J Med Res 1991; 94: 420-423.
32. Piomboni P, Baccetti B. Spermatozoon as a vehicle for HIV-1 and other viruses: a review. Mol Reprod Dev 2000; 56 (Suppl.): 238-242. https://doi.org/10.1002/(SICI)1098-2795(200006)56:2+<238::AID-MRD5>3.0.CO;2-1 [DOI:10.1002/(SICI)1098-2795(200006)56:2+3.0.CO;2-1]
33. Kotronias D, Kapranos N. Detection of herpes simplex virus DNA in human spermatozoa by in situ hybridization technique. In Vivo 1998; 12: 391-394.
34. Hart KM, Murphy AJ, Barrett KT, Wira CR, Guyre PM, Pioli PA. Functional expression of pattern recognition receptors in tissues of the human female reproductive tract. J Reprod Immunol 2009; 80: 33-40. [DOI:10.1016/j.jri.2008.12.004]
35. Ghosh M, Schaefer TM, Fahey JV, Wright JA, Wira CR. Antiviral responses of human Fallopian tube epithelial cells to toll-like receptor 3 agonist poly(I:C). Fertil Steril 2008; 89 (Suppl.): 1497-1506. [DOI:10.1016/j.fertnstert.2007.05.023]
36. Aflatoonian R, Elliott SL, Bruce C, Aflatoonian A, Li TC, Fazeli A. Expression of toll-like receptors in endometrium during the menstrual cycle. J Reprod Immunol 2007; 22: 586-593.
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