Volume 12, Issue 6 (8-2014)                   IJRM 2014, 12(6): 395-0 | Back to browse issues page

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Mahdavipour M, Idali F, Zarei S, Talebi S, Fatemi R, Jeddi-Tehrani M, et al . Investigation on estrogen receptor alpha gene polymorphisms in Iranian women with recurrent pregnancy loss. IJRM 2014; 12 (6) :395-0
URL: http://ijrm.ir/article-1-550-en.html
Investigation on estrogen receptor alpha gene polymorphisms in Iranian women with recurrent pregnancy loss
Marzieh Mahdavipour * 1, Farah Idali2 , Saeed Zarei3 , Saeed Talebi4 , Ramina Fatemi2 , Mahmood Jeddi-Tehrani4 , Somayeh Pahlavan5 , Farzad Rajaei6
1- Cell and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran , farzadraj@yahoo.co.uk
2- Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
3- Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4- Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
5- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
6- Cell and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran
Abstract:   (2345 Views)
Background: Recurrent pregnancy loss (RPL) is a multifactorial disorder. Environmental factors and genetics can affect pregnancy outcomes.
Objective: Conflicting data suggest an association between estrogen receptor alpha (ESR1) gene polymorphisms and RPL. In this study, such association was investigated in Iranian women with RPL.
Materials and Methods: In this case control study, blood samples were collected from 244 women with a history of three or more consecutive pregnancy losses and 104 healthy women with at least two live births. Using polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP), we studied -397C/T and -351A/G polymorphisms on ESR1 gene in case and control subjects.
Results: The genotypic frequencies of -397C/T and -351A/G polymorphisms on ESR1were not significantly different between RPL and control groups (p=0.20 and p=0.09, respectively). A significantly negative correlation was observed between -397C/T and -351A/G (r=-0.852, p<0.001) in RPL women and complete linkage disequilibrium between the investigated polymorphisms was found (D’: 0.959; r-square= 0.758, p<0.001).
Conclusion: This investigation suggests that the analyzed polymorphisms on ESR1gene are not associated with an increased risk of RPL in the studied population.
Keywords: Estrogen receptor, Polymorphism, Abortion, Recurrent.
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Type of Study: Original Article |
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