Volume 12, Issue 9 (10-2014)                   IJRM 2014, 12(9): 623-0 | Back to browse issues page

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Peyghambari F, Amanpour S, Fayazi M, Haddadi M, Muhammadnejad S, Muhammadnejad A, et al . Expression of α4, αv, β1 and β3 integrins during the implantation window on blastocyst of a mouse model of polycystic ovarian syndromes. IJRM 2014; 12 (9) :623-0
URL: http://ijrm.ir/article-1-581-en.html
Expression of α4, αv, β1 and β3 integrins during the implantation window on blastocyst of a mouse model of polycystic ovarian syndromes
Fatemeh Peyghambari1 , Saeid Amanpour2 , Mehri Fayazi3 , Mahnaz Haddadi2 , Samad Muhammadnejad4 , Ahad Muhammadnejad4 , Mehdi Salimi5 , Zohreh Mazaheri * 6
1- Department of Anatomical Sciences, Faculty of Medical Sciences, Islamic Azad University, Yazd Branch, Yazd, Iran
2- Vali-e-Asr Reproductive Health Research Centre, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Anatomical sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
4- Tumor Model Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Physiology, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
6- Department of Anatomical sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran , z_mazaheri@modares.ac.ir
Abstract:   (2480 Views)
Background: It has been hypothesized that blastocyst integrin expression changes can affect the spontaneous miscarriage in polycystic ovarian syndromes (PCOS).
Objective: In this study, the profile of integrin genes and proteins was investigated on blastocyst of the PCOS experimental mouse model.
Materials and Methods: 30 NMRI female mice were equally divided into 3 groups: control, experimental [PCOS that was injected estradiol valerate (40 mg/kg)]. After 8 weeks, each group was hyper stimulated by PMSG and HCG. Vaginal plaque was checked, and mice were investigated 5 days after the test. Progesterone and estradiol levels were determined; α4, αv, β1 and β3 integrin genes and protein of blastocysts were examined by real time PCR method and immunohistochemistry, respectively.
Results: Estradiol level was significantly increased (p≤0.035) in PCOS group. Based on our finding, the ratio of genes' expressions αv, β3, β1 and α4 in PCOS to control group was 0.479±0.01, 0.5±0.001, 2.7±0.4 and 1.023±0.2 respectively. Genes expression showed a great difference (p≤0.001) between β3, β1 and αv in PCOS compared to other groups. αv and β3 integrin proteins expressed in all groups but intensity of these proteins in PCOS groups, was lower than other groups.
Conclusion: Pattern of αv and β3 integrins expression on the mouse blastocyst surface has an important effect during the implantation window. This pattern has changed in PCOS model and might have a great influence on implantation failure. Therefore, this experimental study suggests that a great attention to this problem may be essential in patients who are involved.
Keywords: Integrin, Blastocyst, Implantation, Polycystic ovarian syndromes.
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Type of Study: Original Article |
References
1. Speroff L, Fritz MA. Cronic anovulation and the polycystic ovary syndrome. In: Clinical gynecoligic endocrinology and infertility. Philadelphia 2011; 495-531.
2. Qiao J, Wang L, Li R, Zhang X. Microarray evaluation of endometrial receptivity in chinese women with polycystic ovary syndrome. Reprod Biomed Online 2008; 17: 425-435. [DOI:10.1016/S1472-6483(10)60228-3]
3. Cakmak H, Taylor HS. Human implantation failure: molecular mechanisms and clinical treatment. Hum Reprod Update 2011; 17: 242-253. [DOI:10.1093/humupd/dmq037]
4. Donaghay M, Lessey BA. Uterine receptivity: alterations associated with benign gynecological disease. Semin Reprod Med 2007; 25: 461-475. [DOI:10.1055/s-2007-991044]
5. Lopes IM, Baracat MC, Simões Mde J, Simões RS, Baracat EC, Soares Jr JM. Endometrium in women with polycystic ovary syndrome during the window of implantation. Rev Assoc Med Bras 2011; 57: 702-709.
6. Paria BC, Huet-Hudson YM, Dey SK. Blastocyst's state of activity determines ''window'' of implantation in the receptive mouse uterus. Proc Natl Acad Sci USA 1993; 90: 10159-10162. [DOI:10.1073/pnas.90.21.10159]
7. Aplin J. Adhesion molecules in implantation. Rev Reprod 1997; 2: 84-93. [DOI:10.1530/ror.0.0020084]
8. Harvey MB, Leco KJ, Arcellana-Panlilio MY, Zhang X, Edwards GR, Schultz GA. Roles of growth factors during peri- implantation development. Mol Hum Reprod 1995; 10: 712-718. [DOI:10.1093/oxfordjournals.humrep.a136021]
9. Saito S. Cytokine network at the fetomaternal interface. J Reprod Immunol 2000; 47: 87-103. [DOI:10.1016/S0165-0378(00)00060-7]
10. Chakraborty I, Das SK, Dey SK. Differential expression of vascular endothelial growth factor and its receptor mRNAs in the mouse uterus around the time of implantation. J Endocrinol 1995; 147: 339-352. [DOI:10.1677/joe.0.1470339]
11. Hynes RO. Integrins: versatility, modulation and signaling in cell adhesion. Cell 1992; 69: 11-25. [DOI:10.1016/0092-8674(92)90115-S]
12. Damsky C, Sutherland A, Fisher S. Extracellular matrix 5: adhesive interactions in early mammalian embryogenesis, implantation and placentation. FASEB J 1993; 7: 1320-1329. [DOI:10.1096/fasebj.7.14.8224605]
13. Yoshimura Y. Integrin: expression, modulation and signaling in fertilization, embryogenesis and implantation. Kieo J Med 1997; 46: 16-24. [DOI:10.2302/kjm.46.16]
14. Sutherland AE, Calarco PG, Damsky CH. Developmental regulation of integrin expression at the time of implantation in the mouse embryo. Development 1993; 119: 1175-1186.
15. Aplin J. Adhesion molecules in implantation. Rev Reprod 1997; 2: 84-93. [DOI:10.1530/ror.0.0020084]
16. Reddy KV, Meherji PK. Integrin cell adhesion molecules in endometrium of fertile and infertile women throughout menstrual cycle. Indian J Exp Biol 1999; 37: 323-331.
17. Gonzalez RR, Palomino A, Boric A, Vega M, Devoto L. A quantitative evaluation of a1, a4, aV and b3 endometrial integrins of fertile and unexplained infertile women during the menstrual cycle. A flow cytometric appraisal. Hum Reprod 1999; 14: 2485-2492. [DOI:10.1093/humrep/14.10.2485]
18. Fayazi M, Beigi Brojeni M, Salehnia M, Khansarinejad B. Ovarian stimulation by Exogenous Gonadotropin Decreases the Implantation Rate and Expression of Mouse Blastocysts Integrins. Iran Biomed J 2014; 18: 8-15.
19. Wood JR, Dumesic DA, Abbott DH, Strauss JF 3rd. Molecular Abnormalities in Oocytes from Women with Polycystic Ovary Syndrome Revealed by Microarray Analysis. J Clin Endocrinol Metab 2007; 92: 705-713. [DOI:10.1210/jc.2006-2123]
20. Hou Q, Paria BC, Mui C, Dey SK, Gorsky J. Immunolocalization of estrogen receptor protein in the mouse blastocyst during normal and delayed implantation. Proc Natl Acad Sci USA 1996; 93: 2376-2381. [DOI:10.1073/pnas.93.6.2376]
21. Norman RJ, Dewailly D, Legro RS, Hickey TE. Polycystic ovary syndrome. Lancet 2007; 370: 685-697. [DOI:10.1016/S0140-6736(07)61345-2]
22. Dikmen A, Ergenoglu AM, Yeniel AO, Dilsiz OY, Ercan G, Yilmaz H. Evaluation of glycemic and oxidative/antioxidative status in the estradiol valerate-induced PCOS model of rats. Eur J Obstet Gynecol Reprod Biol 2012; 160: 55-59. [DOI:10.1016/j.ejogrb.2011.09.042]
23. Mahood RAH. Effects of Pimpinella anisum oil Extract on Some Biochemical Parameters in Mice experimentally induced for human Polycystic Ovary Syndrome. J Biotec Res Cent 2012; 6: 67-73.
24. Guyer M. Animal microbiology. 5th Ed. The University of Chicago press, Chicago; 1993.
25. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001; 29: e45. [DOI:10.1093/nar/29.9.e45]
26. Stener-Victorin E, Kobayashi R, Watanabe O, Lundeberg T, Kurosawa M. Effect of electro-acupuncture stimulation of different frequencies and intensities on ovarian blood flow in anaesthetized rats with steroid-induced polycystic ovaries. Reprod Biol Endocrinol 2004; 2: 16. [DOI:10.1186/1477-7827-2-16]
27. Schulster A, Farookhi R, BrawerJ R. Polycystic ovarian condition in Estradiol Valerate-treated rats: spontaneous changes in characteristic endocrine features. Biol Reprod 1984; 31: 587-593. [DOI:10.1095/biolreprod31.3.587]
28. Shirwalker H, Deepak N M, Anurupa M. Exposure of adult rats to estradiol valerate induces ovarian cyst with early senescence of follicles. Mol Cell Endocrinol 2007; 272: 22-37. [DOI:10.1016/j.mce.2007.04.003]
29. Stener-Victorin E, Lundeberg T, Waldenstrom U, Manni L, Aloe L, Gunnarsson S, et al. Effects of Electro-Acupuncture on Nerve Growth Factor and Ovarian Morphology in Rats with Experimentally Induced Polycystic Ovaries. Biol Reprod 2000; 63: 1497-1503. [DOI:10.1095/biolreprod63.5.1497]
30. Luza SM, LizamA L, Burgos RA, Lara HE. Hypothalamic Changes in norepinephrine Release in Rats with EstradiolValerate-Induced Polycystic Ovaries. Biol Reprod 1995; 52: 398-404. [DOI:10.1095/biolreprod52.2.398]
31. Brawer JR, Naftolin F, Martin J, Sonnenschein C. Effects of a single injection of estradiol valerate on the hypothalamic arcuate nucleus and on reproductive function in the female rat. Endocrinology 1978; 103: 501-512. [DOI:10.1210/endo-103-2-501]
32. Peyghambari F, Salehnia M, Forouzandeh Moghadam M, Rezazadeh Valujerdi M, Hajizadeh E. The correlation between the endometrial integrins and osteopontin expression with pinopodes development in ovariectomized mice in response to exogenous steroids hormones. Iran Biomed J 2010; 14: 109-19.
33. Lessey BA, Damjanovich L, Coutifaris C, Castelbaum A, Albelda SM, Buck CA. Integrin adhesion molecules in the human endometrium. Correlation with the normal and abnormal menstrual cycle. J Clin Invest 1992; 90: 188-195. [DOI:10.1172/JCI115835]
34. Widra EA, Weeraratna A, Stepp MA, Stillman RJ, Patierno SR. Modulation of implantation-associated integrin expression but not uteroglobin by steroid hormones in an endometrial cell line. Mol Hum Reprod 1997; 3: 563-568. [DOI:10.1093/molehr/3.7.563]
35. Basak S, Dhar R, Das C. Steroids modulate the expression of alpha4 integrin in mouse blastocysts and uterus during implantation. Biol Reprod 2002; 66: 1784-1789. [DOI:10.1095/biolreprod66.6.1784]
36. Kimmins S, MacLaren LA. Cyclic Modulation of integrin expression in bovine endometrium. Biol Reprod 1999; 61: 1267-1274. [DOI:10.1095/biolreprod61.5.1267]
37. Lessey BA, Arnold JT. Paracrine signaling in the endometrium: integrins and the establishment of uterine receptivity. J Reprod Immunol 1998; 39: 105-116. [DOI:10.1016/S0165-0378(98)00016-3]
38. Apparao KB, Murray MJ, Fritz MA, Meyer WR,Chambers AF, Truong PR, et al. Osteopontin and its receptor alphavbeta (3) integrin are coexpressed in the human endometrium during the menstrual cycle but regulated differentially. J Clin Endocrinol Metab 2001; 86: 4991-5000.
39. Bowen JA, Bazer FW, Burghardt RC. Spatial and temporal analyses of integrin and Muc-1 expression in porcine uterine epithelium and trophectoderm in vivo. Biol Reprod 1996; 55: 1098-1110. [DOI:10.1095/biolreprod55.5.1098]
40. Lin H, Wang X, Liu G, Fu J, Wang A. Expression of alpha V and beta 3 integrin subunits during implantation in pig. Mol Reprod Dev 2007; 74: 1379-1385. [DOI:10.1002/mrd.20732]
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