Volume 12, Issue 9 (10-2014)                   IJRM 2014, 12(9): 601-0 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mohseni Moghadam N, Kheirkhah B, Mirshekari T R, Fasihi Harandi M, Tafsiri E. Isolation and molecular identification of mycoplasma genitalium from the secretion of genital tract in infertile male and female. IJRM 2014; 12 (9) :601-0
URL: http://ijrm.ir/article-1-583-en.html
Isolation and molecular identification of mycoplasma genitalium from the secretion of genital tract in infertile male and female
Naeime Mohseni Moghadam * 1, Babak Kheirkhah2 , Toraj Reza Mirshekari3 , Majid Fasihi Harandi4 , Elham Tafsiri5
1- Department of IVF, Afzalipoor Hospital, IVF Center, Kerman, Iran , N.mohseni@kmu.ac.ir
2- Department of Microbiology, College of Science, Kerman Branch, Islamic Azad University, Kerman, Iran
3- Department of Pathology, Afzalipoor Medical College, Kerman, Iran
4- Department of Parasitology, Afzalipoor Medical College, Kerman, Iran
5- Department of Molecular Medical Genetic, Biotechnology Research Center, Pasteur Institute, Tehran, Iran
Abstract:   (2831 Views)
Background: Mycoplasmas can cause acute and chronic diseases at multiple sites with wide-range complications and have been implicated as cofactors in diseases. The infections influenced form genital mycoplasmas specifically Mycoplasma hominis and Mycoplasma genitalium potentially affect reproductive disorders, and infertility.
Objective: Isolation and molecular identification of Mycoplasma genitalium from the genital tract of infertile male and vaginal discharge of infertile female referred to Infertility Center of Kerman in 2013.
Materials and Methods: This study was a randomized, prospective study. We included 100 infertile male and 100 infertile female that were referred to the Infertility Center of Kerman. Then for isolation and molecular identification of Mycoplasma genitalium from urethral and vaginal discharge polymerase chain reaction was performed on Mycoplasma genus and genitalium.
Results: From a total of 100 semen samples 45 patients (45%) were mycoplasma-positive and 13 (28.8%) were genitalium species positive. Also, from a total of 100 women samples 43 women (43%) were mycoplasma-positive and 10 (23.2%) were genitalium species positive. Positive samples were sequenced and phylogenetic tree was drawn.
Conclusion: According to the results of this study, a high percentage of infertile male and female were infected with the Mycoplasma genitalium. For prevention of harmful and significant consequences of this infection, we suggest a screening program in symptomatic infertile couples.
Keywords: Mycoplasma genitalium, polymerase chain reaction, Asymptomatic Infections, Infertility, Sequence Analysis
Full-Text [PDF 257 kb]   (1118 Downloads) |   |   Full-Text (HTML)  (640 Views)  
Type of Study: Original Article |
References
1. Jonathan Rk, Jayodita CS, Derek NM, Miriam VG, Jared MJ, Bolival B, et al. Infected men with nongonococcal urethritis attending STD & HIV clinics. Indian J Med Res 2009; 129: 305-310.
2. Berger RE, Karp LE, Williamson RA, Koehler J, Moore DE, Holmes KK, et al. The relationship of pyospermia and seminal fluid bacteriology to sperm function as reflected in the sperm penetration assay. Fertil Steril 1982; 37: 557-564. [DOI:10.1016/S0015-0282(16)46166-2]
3. Debata NK, Naessens A, Sanocka M. Prevalence of mycoplasmas in genital tract. Indian J Med Microbiol 1990; 15: 25-27.
4. Reid I, Martínez MA, Birón M, Aceituno R. Mycoplasmahominis and Ureaplasma urealyticum in pregnant and infertilewomen. Rev Chil Obstet Ginecol 1993; 58: 349-354.
5. Speroff GK. Williams and Wilkins Gynecology. Endocrinol Infertil 1994; 68: 530-533.
6. Szeredi L, Tenk M, Schiller I, Révész T. Study of the role of Chlamydia, Mycoplasma, Ureaplasma and other microaerophilic and aerobic bacteria in uterine infections of mares with reproduction disorder. Acta Vet Hung 2003; 51: 45-52. [DOI:10.1556/AVet.51.2003.1.4]
7. Khalili MA. Assessment of bacterial infection on sperm quality in infertile men. Proceedings of the Fourth Congress of Microbiology 2001: 49. (In Persian)
8. Svenstrup HF, Fedder J, Abraham-peskis J, Brikelund S, Christiansen G. Mycoplasma genitalium attaches to human spermatozoa. Hum Reprod 2003; 18: 2103-2109. [DOI:10.1093/humrep/deg392]
9. World Health Organization (WHO). World Health Organization (WHO) laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4PthP Ed. Cambridge University Inc, Cambridge, UK; 1999: 1-138.
10. Kojima A, Takahashi T, Kijima M, Ogikubo Y, Nishimura Y, Nishimura S, et al. Detection of Mycoplasma in live virus vaccines by polymerase chain reaction. Biologicals 1997; 25: 365-371. [DOI:10.1006/biol.1997.0108]
11. Jensen JS, Borre MB, Dohn B. Detection of Mycoplasma genitalium by Amplification of the 16srRNA Gene. J Clin Microbiol 2003; 41: 261-266. [DOI:10.1128/JCM.41.1.261-266.2003]
12. Bakhshande Nosrat S, Ghazi Saeidi K, Livani S, Dadgar T, Bazori M, Bagheri H, et al. Distribution of genital mycoplasmas in vaginal infection in Gorgan, Iran. Iran J Obstet Gynecol Infertil 1389; 14: 20-28. (In Persian)
13. Amirmozaffari N, Ahmadi M, Sedighi Gilani M, Kazemi B, Masjedian Jazi F. Detection of Mycoplasma Hominis and Ureaplasma Urealyticum from Semen Samples of Infertile Men Referred to Royan Institute in 2008. RJMS 2010; 17: 14-26. (In Persian)
14. Soleimani Rahbar A, Golshani M, Fayyaz F, Rafiee Tabatabaei S, Moradi A. Detection of Mycoplasma DNA from the sperm specimens of infertile men by PCR. Iran J Med Microbiol 2007; 1: 47-53. (In Persian)
15. Manhas A, Sethi S, Sharma M, Wanchu A, Kanwar AJ, Kaur K, Mehta SD. Association of genital mycoplasmas includingMycoplasma genitalium in HIV infected men with nongonococcal urethritis attending STD & HIV clinics. Indian J Med Res 2009; 129: 305-310.
16. Vatani SH, Ghazisaidi K, Mohamadi M, Naji AR, Fateminasab F, Zeraati H, et al. The survey of contmination with genital mycoplasma in women with bactrial vaginalis by PCR method. J Gorgan Uni Med Sci 2006; 8: 45-50. (In Persian)
17. Larsen B, Hwang J. Mycoplasma, Ureaplasma, and Adverse Pregnancy Outcomes. Infect Dis Obstet Gynecol 2010; 2010: 521921. [DOI:10.1155/2010/521921]
18. Vosooghi S, kheirkhah B, kariminik A, mirshekari T. A review of the role of Mycoplasma infections in humans' infertility. NCMBJ 2012; 2: 9-20. (In Persian)
19. Nik Pour S, Tabrizian L, Daryadokht Masroor Roodsari, Haghani HStudy of predisposing factor of urinary tract infections among married women referred to selected hospitals in Tehran city, 2003. Razi J Med Sci 2004; 11: 498-489. (In Persian)
20. Ahmadi M, Amirmozafari N, Sedighi-Gilani M, Kazemi B, Masjedian-Jazi F. Comparison of Culture with PCR for Detection of Mycoplasma hominis and Ureaplasma urealyticum in Semen Samples of Infertile Men Referring to the Royan Institute in 2009. RJMS 2010; 17: 15-29. (In Persian)
21. Stellrecht KA, Woron AM, Mishrik NG, Venezia RA. Comparison of multiplex PCR assay with culture for detection of genital mycoplasmas. J Clin Microbiol 2004; 42: 1528-1533. [DOI:10.1128/JCM.42.4.1528-1533.2004]
22. Mousaviyan M, Pordeli H. Survey of respiratory and urogenital infections due to Mycoplasma in the hospitalized patients in Ahwaz Imam Khomeini Hospital. J Kerman Univ Med Sci 2003; 10: 1-10. (In Persian)
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb