Volume 13, Issue 10 (10-2015)                   IJRM 2015, 13(10): 627-632 | Back to browse issues page

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Shakarami F, Taghi Akbari M, Zare Karizi S. Association of plasminogen activator inhibitor-1 (PAI-1) and angiotensin converting enzyme polymorphisms with recurrent pregnancy loss in Iranian women. IJRM 2015; 13 (10) :627-632
URL: http://ijrm.ir/article-1-595-en.html
Association of plasminogen activator inhibitor-1 (PAI-1) and angiotensin converting enzyme polymorphisms with recurrent pregnancy loss in Iranian women
Fatemeh Shakarami1 , Mohammad Taghi Akbari * 2, Shohreh Zare Karizi3
1- Department of Biology, Guilan University, Rasht, Iran
2- Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , mtakbari@modares.ac.ir
3- Department of Biology, Varamin Pishva Branch, Islamic Azad University, Varamin Pishva, Iran
Abstract:   (3503 Views)
Background: Recurrent pregnancy loss (RPL) defined by two or more failed pregnancies before 20 weeks of gestation. Several factors play a role in RPL including thrombophilic conditions which can be influenced by gene polymorphisms. Plasminogen activator inhibitor-1 (PAI-1) and angiotensin converting enzyme (ACE) genes are closely related to fibrinolytic process, embryonic development and pregnancy success.
Objective: The aim of this study was to investigate the relationship between RPL and common polymorphisms in ACE and PAI-1 genes.
Materials and Methods: In this case control study, 100 women with recurrent abortions (at least two) were selected as cases and 100 healthy women with two or more normal term deliveries without a history of abortion as controls. Total genomic DNA was isolated from blood leukocytes. The status of the PAI-1 4G/5G and ACE (D/I) polymorphism was determined by PCR-RFLP.
Results: Homozygosity for PAI-1 4G polymorphism was seen in 17 cases (17%), and 5 controls (5%) (p=0.006) so patients with homozygote 4G mutation were significantly more prone to RPL in contrast to control group (OR: 4.63, % 95 CI: 1.55-13.84). In addition, 7 patients (7 %), and no one from the control group, were homozygote (I/I) for ACE polymorphism (p=0.034), suggesting no significant associations between ACE D allele or DD genotype and RPL.
Conclusion: Considering these results, because 4G/4G polymorphism for PAI-1 gene could be a thrombophilic variant leading to abortion, analysis of this mutation and other susceptibility factors are recommended in patients with RPL.
Keywords: Spontaneous abortion, Thrombophilia, Plasminogen activator inhibitor-1, Angiotensin converting enzyme
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Type of Study: Original Article |
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