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Volume 5, Issue 2 (7-2007)
IJRM 2007, 5(2): 23-28 |
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Farsi M M, Jorsaraei A, Hajiahmadi M, Esmaelzadeh S. Role of embryo morphology in Intracytoplasmic Sperm Injection cycles for prediction of pregnancy. IJRM 2007; 5 (2) :23-28
URL: http://ijrm.ir/article-1-60-en.html
URL: http://ijrm.ir/article-1-60-en.html
1- Babol University of Medical Sciences, Babol, Iran , m_farsica@ yahoo.com
2- Babol University of Medical Sciences, Babol, Iran
2- Babol University of Medical Sciences, Babol, Iran
Abstract: (2903 Views)
Background: Multiple factors have been suggested for prediction of pregnancy in Intracytoplasmic sperm injection (ICSI) cycles such as the number of injected oocytes, fertilization rate, embryo morphology and quality of transferred embryos. Predictive value of these factors is important in ICSI outcome.
Objectives: To evaluate the role of embryo morphology for prediction of pregnancy in ICSI cycles.
Materials and Methods: This retrospective study was done on 97 patients who were treated by ICSI in Fatemeh Zahra Fertility and Infertility Centre from April 2004 to March 2005. Number of retrieved oocytes, number of injected oocytes, fertilization rate, zygote morphology, rate of cytoplasmic fragmentation, number of four cell transferred embryos, and quality of embryo transfer, as predictors of pregnancy in ICSI cycles were evaluated. The results analysed by T-test, Mann-Whitney U test and Fisher's exact test. Logistic regression was used to estimate the significance of variables in the prediction of pregnancy probability.
Results: Out of 97 patients, 42 cases of pregnancy were detected (Pregnancy rate: 43.3%). The number of four cell transferred embryos was 112 (53.84%) in pregnant group. Pregnancy occurred in 33 (58.9%) patients with at least one good quality zygote. The mean number of four cell transferred embryos and the quality of zygotes had significant difference between pregnant and not pregnant groups (p=0.006 and p=0.000 respectively). In logistic regression analysis, the number of four-cell transferred embryos (p=0.007) and the quality of zygotes (p=0.003) were significant predictors of the pregnancy outcome.
Conclusions: Our results suggest that the number of four-cell transferred embryos with ≤ 15% cytoplasmic fragmentation and zygotes with centralized, apposed and polarized pronuclei in women <38 years old are significant predictors for pregnancy in ICSI cycles.
Objectives: To evaluate the role of embryo morphology for prediction of pregnancy in ICSI cycles.
Materials and Methods: This retrospective study was done on 97 patients who were treated by ICSI in Fatemeh Zahra Fertility and Infertility Centre from April 2004 to March 2005. Number of retrieved oocytes, number of injected oocytes, fertilization rate, zygote morphology, rate of cytoplasmic fragmentation, number of four cell transferred embryos, and quality of embryo transfer, as predictors of pregnancy in ICSI cycles were evaluated. The results analysed by T-test, Mann-Whitney U test and Fisher's exact test. Logistic regression was used to estimate the significance of variables in the prediction of pregnancy probability.
Results: Out of 97 patients, 42 cases of pregnancy were detected (Pregnancy rate: 43.3%). The number of four cell transferred embryos was 112 (53.84%) in pregnant group. Pregnancy occurred in 33 (58.9%) patients with at least one good quality zygote. The mean number of four cell transferred embryos and the quality of zygotes had significant difference between pregnant and not pregnant groups (p=0.006 and p=0.000 respectively). In logistic regression analysis, the number of four-cell transferred embryos (p=0.007) and the quality of zygotes (p=0.003) were significant predictors of the pregnancy outcome.
Conclusions: Our results suggest that the number of four-cell transferred embryos with ≤ 15% cytoplasmic fragmentation and zygotes with centralized, apposed and polarized pronuclei in women <38 years old are significant predictors for pregnancy in ICSI cycles.
Keywords: Intracytoplasmic sperm injection, Pregnancy prediction, Zygote morphology, Four-cell transferred embryos
Type of Study: Original Article |
References
1. Gunalp S, Onculoglu C, Gurgan T, Kruger TF, Lombard CJ. A study of semen parameters with emphasis on sperm morphology in a fertile population: an attempt to develop clinical thresholds. Hum Reprod 2001; 16: 110-114. [DOI:10.1093/humrep/16.1.110]
2. Menkveld R, Wong WY, Lombard CJ, Wetzels AM, Thomas CM, Merkus HM, et al. Semen parameters, including WHO and strict criteria morphology, in a fertile and subfertile population: an effort towards standardization of in-vivo thresholds. Hum Reprod 2001; 16: 1165-1171. [DOI:10.1093/humrep/16.6.1165]
3. Oehninger S, Chaturvedi S, Toner J, Morshedi M, Mayer J, Lanzendorf S, et al. Semen quality: Is there a paternal effect on pregnancy outcome in in-vitro fertilization/ intracytoplasmic sperm injection? Hum Reprod 1998; 13: 2161-2164. [DOI:10.1093/humrep/13.8.2161]
4. Spandorfer SD, Avrech OM, Colombero LT, Palermo GD, Rosenwaks Z. Effect of parental age on fertilization and pregnancy characteristics in couples treated by intracytoplasmic sperm injection. Hum Reprod 1998; 13: 334-338. [DOI:10.1093/humrep/13.2.334]
5. Shen S, Khabani A, Klein N, Battaglia D. Statistical analysis if factors affecting fertilization rates and clinical outcome associated with intracytoplasmic sperm injection. Fertil steril 2003; 79: 355-360. [DOI:10.1016/S0015-0282(02)04675-7]
6. Garello C, Baker H, Rai J, Montgomery S, Wilson P, Kennedy CR, Hartshorne GM. Pronuclear orientation, polar body placement, and embryo quality after intracytoplasmic sperm injection and in-vitro fertilization: further evidence for polarity in human oocytes? Hum Reprod 1999; 14: 2588-2595. [DOI:10.1093/humrep/14.10.2588]
7. Kahraman S, Kumtepe Y, Sertyel S, Donmez E, Benkhalifa M, Findikli N, et al. Pronuclear morphology scoring and chromosomal status of embryos in severe male infertility. Hum Reprod 2002; 17: 3193-200. [DOI:10.1093/humrep/17.12.3193]
8. Tesarik J, Greco E. The probability of abnormal preimplantation development can be predicted by a single static observation on pronuclear stage morphology. Hum Reprod 1999; 14: 1318-1323. [DOI:10.1093/humrep/14.5.1318]
9. Sennl A, Urner F, Chanson A, Primi MP, Writhner D, Germond M. Morphological scoring of human pronuclear zygotes for prediction of pregnancy outcome. Hum Reprod 2006; 21: 234-239. [DOI:10.1093/humrep/dei282]
10. Fisch JD, Rodriguez H, Ross R, Overby G, Sher G. The Graduated Embryo Score (GES) predicts blastocyst formation and pregnancy rate from cleavage-stage embryos. Hum Reprod 2001; 16: 1970-1975. [DOI:10.1093/humrep/16.9.1970]
11. Saldeen P, Sundstrom P. Nuclear status of four-cell pre-embryos predicts implantation potential in invitro fertilization treatment cycles. Fertil Steril 2005; 84: 584-589. [DOI:10.1016/j.fertnstert.2005.03.059]
12. Zollner U, Zollner KP, Hartl G, Dietl J, Steck T. The use of a detailed zygote score after IVF/ICSI to obtain good quality blastocysts: the German experience. Hum reprod 2002; 17: 1327-1333. [DOI:10.1093/humrep/17.5.1327]
13. Balaban B, Urman B, Isiklar A, Alatas C, Aksoy S, Mercan R, et al. The effect of pronuclear morphology on embryo quality parameters and blastocyst transfer outcome. Hum Reprod 2001; 16: 2357-2361. [DOI:10.1093/humrep/16.11.2357]
14. Placido GD, Wilding M, Strina I, Alviggi E, Alviggi C, Mollo A, et al. High outcome predictability after IVF using a combined score for zygote and embryo morphology and growth rate. Hum Reprod 2002; 17: 2402-2409. [DOI:10.1093/humrep/17.9.2402]
15. Balaban B, Urman B. Embryo culture as a diagnostic tool. Reprod Biomed online 2003; 7: 671-682. [DOI:10.1016/S1472-6483(10)62090-1]
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