Volume 13, Issue 12 (12-2015)                   IJRM 2015, 13(12): 787-792 | Back to browse issues page

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Ghyasvand T, Goodarzi M T, Amiri I, Karimi J, Ghorbani M. Serum levels of lycopene, beta-carotene, and retinol and their correlation with sperm DNA damage in normospermic and infertile men. IJRM 2015; 13 (12) :787-792
URL: http://ijrm.ir/article-1-611-en.html
Serum levels of lycopene, beta-carotene, and retinol and their correlation with sperm DNA damage in normospermic and infertile men
Taiebeh Ghyasvand1 , Mohammad Taghi Goodarzi * 2, Iraj Amiri3 , Jamshid Karimi1 , Marzieh Ghorbani3
1- Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, Iran
2- Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran , mt.goodarzi@umsha.ac.ir
3- Endometer and Endometriosis Centre, Fatemieh Hospital, Hamadan University of Medical Sciences, Hamadan, Iran
Abstract:   (3428 Views)
Background: Oxidative stress in reproductive system leads to sperm DNA damage and sperm membrane lipid peroxidation and may play an important role in the pathogenesis of male infertility, especially in idiopathic cases. Antioxidants such as carotenoids function against free radical damages. Objective : The aim of this study was to determine the levels of lycopene, beta-carotene and retinol in serum and their relationship with sperm DNA damage and lipid peroxidation in infertile and normospermic males. Materials and Methods: Sixty two infertile men and 71 normospermic men participated in this study. Blood and semen samples were collected from all subjects. Sperm DNA damage was measured using TUNEL method. Carotenoids, retinol, and malonedildehyde in serum were also determined. Results: DNA fragmentation was higher in infertile group comparing to control group. Serum levels of lycopene, beta-carotene and, vitamin A in infertile men were significantly lower than normospermic men (p< 0.001, =0.005, and =0.003 respectively). While serum MDA was not significantly different between two groups, MDA in seminal plasma of infertile men was significantly higher than control group (p< 0.001). Conclusion: We concluded that lycopene, beta-carotene, and retinol can reduce sperm DNA fragmentation and lipid peroxidation through their antioxidant effect. Therefore the DNA fragmentation assay and determination of antioxidants factors such as lycopene, beta-carotene and retinol, along with sperm analysis can be useful in diagnosis and treatment of men with idiopathic infertility.
Keywords: Infertility, Lycopene, Beta- carotene, Retinol, DNA damage, Lipid peroxidation, Malondialdehyde
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Type of Study: Original Article |
References
1. Ko EY, Siddiqi K, Brannigan RE, Sabanegh ES Jr. Empirical medical therapy for idiopathic male infertility: a survey of the American Urological Association. J Urol 2012; 187: 973-978. [DOI:10.1016/j.juro.2011.10.137]
2. Kessopoulou E, Tomlinson MJ, Barratt CL, Bolton AE, Cooke ID. Origin of reactive oxygen species in human semen: spermatozoa or leucocytes? J Reprod Fertil 1992; 94: 463-470. [DOI:10.1530/jrf.0.0940463]
3. Agarwal A, Saleh RA. Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am 2002; 29: 817–827. [DOI:10.1016/S0094-0143(02)00081-2]
4. de Lamirande E, Jiang H, Zini A, Kodama H, Gagnon C. Reactive oxygen species and sperm physiology. Rev Reprod 1997; 2: 48-54. [DOI:10.1530/ror.0.0020048]
5. Aitken J, Krausz C, Buckingham D. Relationships between biochemical markers for residual sperm cytoplasm, reactive oxygen species generation, and the presence of leukocytes and precursor germ cells in human sperm suspensions. Mol Reprod Dev 1994; 39: 268–279. [DOI:10.1002/mrd.1080390304]
6. Cooke MS, Evans MD, Dizdaroglu M, Lunec J. Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J 2003; 17: 1195-1214. [DOI:10.1096/fj.02-0752rev]
7. Twigg J, Irvine DS, Houston P, Fulton N, Michael L, Aitken RJ. Iatrogenic DNA damage induced in human spermatozoa during sperm preparation: Protective significance of seminal plasma. Mol Hum Reprod 1998; 4: 439–445. [DOI:10.1093/molehr/4.5.439]
8. Kamiya, H. Mutagenic potentials of damaged nucleic acids produced by reactive oxygen/nitrogen species: approaches using synthetic oligonucleotides and nucleotides: survey and summary. Nucleic Acids Res 2003; 31: 517-531. [DOI:10.1093/nar/gkg137]
9. Johnson EJ. The role of carotenoids in human health. Nutr Clin Care 2002; 5: 56-65. [DOI:10.1046/j.1523-5408.2002.00004.x]
10. Agarwal S, Rao AV. Carotenoids and chronic diseases. Drug Metabol Drug Interact 2000; 17: 189–210. [DOI:10.1515/DMDI.2000.17.1-4.189]
11. Elliott R. Mechanisms of genomic and non-genomic actions of carotenoids. Biochim Biophys Acta 2005; 1740: 147–154. [DOI:10.1016/j.bbadis.2004.12.009]
12. Ribaya-Mercado JD, Blumberg JB. Lutein and zeaxanthin and their potential roles in disease prevention. J Am Coll Nutr 2004; 23: 567S–587S. [DOI:10.1080/07315724.2004.10719427]
13. Arab L, Steck S. lycopene and cardiovascular disease. Am J Clin Nutr 2000; 71: 1691s-1695s.
14. Boileau AC, Merchen NR, Wasson K, Atkinson, Erdman JW Jr. Cis-lycopene is more bioavailable than trans-lycopene in vitro and in vivo in lymph-cannulated ferrets. J Nutr 1999; 129: 1176-1181.
15. Hogarth CA, Griswold MD. The key role of vitamin A in spermatogenesis. J Clin Invest 2010; 120: 956–962. [DOI:10.1172/JCI41303]
16. Dandekar SP, Nadkarni GD, Kulkarni VS, Punekar S. Lipid peroxidation and antioxidants enzymes in male infertility. J Postgrad Med 2002; 48: 186-189.
17. World Health Organization. WHO laboratory manual for the examination of human semen and spermcervical mucus interaction, 4th ed. New York: Cambridge University Press 1999; 218-220.
18. Talwara D, Ha TK, Cooneya J, Brownlee C, O'Reilly DS. A routine method for the simultaneous measurement of retinol, alpha-tocopherol and five carotenoids in human plasma by reverse phase HPLC. Clin Chim Acta 1998; 270: 85–100. [DOI:10.1016/S0009-8981(97)00224-6]
19. Neyestani TR, Shariatzadeh N, Gharavi A, Kalayi A, Khalaji N. Physiological dose of lycopene suppressed oxidative stress and enhanced serum levels of immunoglobin in patients with type 2 diabetes mellitus: A possible role in the prevention of long term complications. J Endocrin Invest 2007; 30: 833-838. [DOI:10.1007/BF03349224]
20. Mehrotra A, Katiyar DK, Agarwal A, Das V, Pant KK. Role of total antioxidant capacity and lipid peroxidation in fertile and infertile men. Biomed Res 2013; 24: 347-352.
21. Tavilani H, Doosti M, Saeidi H. Malondialdehyde levels in sperm and seminal plasma of asthenozoospermic and its relationship with semen parameters. Clin Chim Acta 2005; 356: 199-203. [DOI:10.1016/j.cccn.2005.01.017]
22. Ben Abdallah F, Dammak L, Attia H, Hentati B, Ammar-Keskes L. Lipid Peroxidation and Antioxidant Enzyme Activities in Infertile Men: Correlation With Semen Parameter. J Clin Lab Anal 2009; 23: 99–104. [DOI:10.1002/jcla.20297]
23. Agarwal A, Virk G, Ong C, du Plessis SS. Effect of Oxidative Stress on Male Reproduction. World J Mens Health 2014; 32: 1-17. [DOI:10.5534/wjmh.2014.32.1.1]
24. Alizadeh Nili H, Mozdarani H, Pellestor F. Impact of DNA damage on the frequency of sperm Chromosomal aneuploidy in normal and subfertile men. Iran Biomed J 2011; 15: 122-129.
25. Takeda K, Uchiyama K, Kinukawa M, Tagami T, Kaneda M, Watanabe S. Evaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen quality. J Reprod Dev 2015; 61: 185-190. [DOI:10.1262/jrd.2014-140]
26. Sharma RK, Sabanegh E, Mahfouz R. Gupta S, Thiyagarajan A, Agarwal A. TUNEL as a test for sperm DNA damage in the evaluation of male infertility. Urology 2010; 76: 1380-1386. [DOI:10.1016/j.urology.2010.04.036]
27. Sergerie M, Laforest G, Bujan L, Bissonnette F, Bleau G. Sperm DNA fragmentation: threshold value in male fertility. Hum Reprod 2005; 20: 3446-3451. [DOI:10.1093/humrep/dei231]
28. Trisini AT, Singh NP, Duty SM, Hauser R. Relationship between human semen parameters and deoxyribonucleic acid damage assessed by the neutral comet assay. Fertil Steril 2004; 82: 1623-1632. [DOI:10.1016/j.fertnstert.2004.05.087]
29. Zini A, Bielecki R, Phang D, Zenzes MT. Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril 2001; 75: 674-677. [DOI:10.1016/S0015-0282(00)01796-9]
30. Loft S, Kold-Jensen T, Hjollund NH, Giwercman A, Gyllemborg J, Ernst E, et al. Oxidative DNA damage in human sperm influences time to pregnancy. Hum Reprod 2003; 18: 1265-1272. [DOI:10.1093/humrep/deg202]
31. Carrell DT, Liu L, Peterson CM, Jones KP, Hatasaka HH, Erickson L, et al. Sperm DNA fragmentation is increased in couples with unexplained recurrent pregnancy loss. Arch Androl 2003; 49: 49-55. [DOI:10.1080/01485010290099390]
32. Rao AV. Processed tomato products as a source of dietary lycopene: bioavailability and antioxidant properties. Can J Diet Pract Res 2004; 65: 161–165. [DOI:10.3148/65.4.2004.161]
33. Allen CM, Smith AM, Clinton SK, Schwartz SJ. Tomato consumption increases lycopene isomer concentrations in breast milk and plasma of lactating women. J Am Diet Assoc 2002; 102: 1257–1262. [DOI:10.1016/S0002-8223(02)90278-6]
34. Goyal A, Chopra M, Lwaleed BA, Birch B, Cooper AJ. The effects of dietary lycopene supplementation on human seminal plasma. BJU Int 2007; 99: 1456-1460. [DOI:10.1111/j.1464-410X.2007.06804.x]
35. Gupta NP, Kumar R. Lycopene therapy in idiopathic male infertility – a preliminary report. Int Urol Nephrol 2002; 34: 369–372. [DOI:10.1023/A:1024483520560]
36. Palini S, De Stefani S, Dott Benedetti S, Bulletti DC, Canestrari F. Analysis of the correlation between semen parameters and the levels of retinol, tocopherol and carotenoids in human seminal plasma and blood. Fertil Steril 2011; 96: 457. [DOI:10.1016/j.fertnstert.2011.07.926]
37. Lewis SE. Sterling ES, Young IS, Thompson W. Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men. Fertil Steril 1997; 67: 142-147. [DOI:10.1016/S0015-0282(97)81871-7]
38. Zini A, San Gabriel M, Libman J. Lycopene supplementation in vitro can protect human sperm deoxyribonucleic acid from oxidative damage. Fertil Steril 2010; 94: 1033-1036. [DOI:10.1016/j.fertnstert.2009.04.004]
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