Volume 13, Issue 2 (2-2015)                   IJRM 2015, 13(2): 71-78 | Back to browse issues page

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Shahnazi V, Zaree M, Nouri M, Mehrzad-Sadaghiani M, Fayezi S, Darabi M, et al . Influence of ω-3 fatty acid eicosapentaenoic acid on IGF-1 and COX-2 gene expression in granulosa cells of PCOS women. IJRM 2015; 13 (2) :71-78
URL: http://ijrm.ir/article-1-622-en.html
Influence of ω-3 fatty acid eicosapentaenoic acid on IGF-1 and COX-2 gene expression in granulosa cells of PCOS women
Vahideh Shahnazi1 , Mina Zaree2 , Mohammad Nouri1 , Mahzad Mehrzad-Sadaghiani1 , Shabnam Fayezi3 , Maryam Darabi4 , Sajjad Khani5 , Masoud Darabi * 6
1- Women’s Reproductive Health Research Center, Al-zahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
2- Department of Biochemistry and Clinical Laboratories, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3- Students Research Committee, Infertility and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Drug Applied Research Center of Tabriz University of Medical Sciences, Tabriz, Iran
5- Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
6- Women’s Reproductive Health Research Center, Al-zahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran , darabim@tbzmed.ac.ir
Abstract:   (2743 Views)
Background: The omega-3 (ω-3) fatty acid eicosapentaenoic acid (EPA) is currently used in the clinic as a nutritional supplement to improve infertility, particularly in women with polycystic ovarian syndrome (PCOS).
Objective: The present study was designed to investigate the effect of EPA on insulin-like growth factor 1 (IGF-1) and cyclooxygenase 2 (COX-2) gene expression in primary cultured granulosa cells from patients undergoing in vitro fertilization (IVF), and also to compare this effect with those in granulosa cells of PCOS patients.
Materials and Methods: In this experimental study, human granulosa cells were isolated from follicular fluid of normal and PCOS women undergoing IVF by hyaluronidase digestions, followed by Percoll gradient centrifugation. Cells were cultured in vitro, exposed to a range of concentrations of the EPA (25-100 μM) for 24 hr, and investigated with respect to COX-2 and IGF-1 gene expression by real time-PCR.
Results: In both groups, all doses of the EPA significantly induced IGF-1 mRNAgene expression compared to the untreated control. High doses of EPA in thepresence of recombinant (r) FSH produced a stimulatory effect on IGF-1 and asuppressive effect (p=0.01) on the COX-2 gene expression, which were morepronounced in granulosa cells from PCOS patients.
Conclusion: EPA affect diversely the gene expression of IGF-1 and COX-2 in granulosa cells, which were more pronounced in PCOS compared to control. These findings represent the possible underlying molecular mechanisms for the positive impact of the ω-3 fatty acids on reproduction, especially in patients with PCOS.
Keywords: Eicosapentaenoic acid, Insulin-like growth factor 1, Cyclooxygenase 2, Granulosa cells, Polycystic ovary syndrome.
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Type of Study: Original Article |
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