Volume 13, Issue 5 (7-2015)                   IJRM 2015, 13(5): 269-274 | Back to browse issues page

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Atabaki pasdar F, Khooei A, Fazel A, Rastin M, Tabasi N, Peirouvi T et al . DNA flow cytometric analysis in variable types of hydropic placentas. IJRM 2015; 13 (5) :269-274
URL: http://ijrm.ir/article-1-650-en.html
DNA flow cytometric analysis in variable types of hydropic placentas
Fatemeh Atabaki pasdar1 , Alireza Khooei2 , Alireza Fazel3 , Maryam Rastin4 , Nafise Tabasi4 , Tahmineh Peirouvi1 , Mahmoud Mahmoudi * 5
1- Department of Anatomical Sciences, Urmia University of Medical Sciences, Urmia, Iran
2- Department of Pathology, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
3- Department of Anatomy and Cell Biology, Mashhad University of Medical Sciences, Mashhad, Iran
4- Immunology Research Center, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
5- Immunology Research Center, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran , Mahmoudim@mums.ac.ir
Abstract:   (4893 Views)
Background: Differential diagnosis between complete hydatidiform mole, partial hydatidiform mole and hydropic abortion, known as hydropic placentas is still a challenge for pathologists but it is very important for patient management.
Objective: We analyzed the nuclear DNA content of various types of hydropic placentas by flowcytometry.
Materials and Methods: DNA ploidy analysis was performed in 20 non-molar (hydropic and non-hydropic spontaneous abortions) and 20 molar (complete and partial moles), formalin-fixed, paraffin-embedded tissue samples by flow cytometry. The criteria for selection were based on the histopathologic diagnosis.
Results: Of 10 cases histologically diagnosed as complete hydatiform mole, 9 cases yielded diploid histograms, and 1 case was tetraploid. Of 10 partial hydatidiform moles, 8 were triploid and 2 were diploid. All of 20 cases diagnosed as spontaneous abortions (hydropic and non-hydropic) yielded diploid histograms.
Conclusion: These findings signify the importance of the combined use of conventional histology and ploidy analysis in the differential diagnosis of complete hydatidiform mole, partial hydatidiform mole and hydropic abortion.
Keywords: Flow cytometry, Hydatidiform mole, Spontaneous abortion, Ploidy.
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Type of Study: Original Article |
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