Volume 13, Issue 9 (10-2015)                   IJRM 2015, 13(9): 557-562 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rahiminejad M E, Moaddab A, Ebrahimi M, Rabiee S, Zamani A, Ezzati M et al . The relationship between some endometrial secretion cytokines and in vitro fertilization. IJRM 2015; 13 (9) :557-562
URL: http://ijrm.ir/article-1-680-en.html
The relationship between some endometrial secretion cytokines and in vitro fertilization
Mohammad Ehsan Rahiminejad1 , Amirhossein Moaddab * 2, Mehrnoosh Ebrahimi1 , Soghra Rabiee1 , Alireza Zamani3 , Mohammad Ezzati4 , Alireza Abdollah Shamshirsaz5
1- Department of Obstetrics & Gynecology, Hamadan University of Medical Sciences and Health Services, Hamadan, Iran
2- Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas, USA , moaddab@bcm.edu
3- Department of Immunology and Hematology, Hamadan University of Medical Sciences and Health Services, Hamadan, Iran
4- Department of Obstetrics and Gynecology, UT Southwestern Medical Center, Dallas, TX, USA
5- Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas, USA
Abstract:   (2733 Views)
Background: Endometrial secretion analysis is a non-invasive and promising method in evaluation of endometrial receptivity.
Objective: The aim of the present study was to assess the relationship between the success rate of IVF procedures and some endometrial secretion cytokines, including interleukin-1β (IL-1β), tumor necrosis factor (TNF-α), interferon gamma-induced protein 10 (IP-10), and monocyte chemoattractant protein (MCP).
Materials and Methods: In a prospective cohort study, 50 women selected for IVF met the study inclusion criteria. All the patients underwent endometrial secretion aspiration prior to embryo transfer. The level of IL-1β, TNF-α, IP-10 and MCP were analyzed by enzyme-linked immunosorbent assay method using special standard kits. To detect successful implantation and pregnancy patients underwent serum human chorionic gonadotropin measurements and ultrasound evaluation.
Results: Five samples were excluded. Nine women (20%) had successful clinical pregnancies, which resulted in live birth. Other 36 women (80%) were classified as failed pregnancy. Comparison of cytokine levels showed lower concentrations of TNF-α, IP-10, and MCP in the group with successful clinical pregnancy compared to the group with failed pregnancy (p=0.007, 0.005 and 0.001, respectively). However, no significant difference was revealed in IL-1β levels between two groups (p=0.614).
Conclusion: The current study suggested that lower concentrations of TNF-α, IP-10, and MCP in endometrial secretions might be associated with improved endometrial receptivity and IVF outcome. Regarding IL-1β, no statistically significant differences were seen between the groups with and without successful pregnancy.
Keywords: Cytokine, In vitro fertilization, Outcome, Endometrium, Implantation.
Full-Text [PDF 136 kb]   (935 Downloads) |   |   Full-Text (HTML)  (381 Views)  
Type of Study: Original Article |
References
1. Boomsma CM, Kavelaars A, Eijkemans MJ, Fauser BC, Heijnen CJ, Macklon NS. Ovarian stimulation for in vitro fertilization alters the intrauterine cytokine, chemokine, and growth factor milieu encountered by the embryo. Fertil Steril 2009; 94: 1764-1768. [DOI:10.1016/j.fertnstert.2009.10.044]
2. Campbell KL, Rockett JC. Biomarkers of ovulation, endometrial receptivity, fertilization, implantation and early pregnancy progression. Paediatr Perinat Epidemiol 2006; 20: 13-25. [DOI:10.1111/j.1365-3016.2006.00766.x]
3. Cavagna M, Mantese JC. Biomarkers of endometrial receptivity-a review. Placenta 2003; 24: 39-47. [DOI:10.1016/S0143-4004(03)00184-X]
4. Dikareva LV, Shvarev EG, Shvarev GE, Teplyi DL. Age, structural and biochemical characteristics of endometrial secretion in patients with hysteromyoma. Adv Gerontol 2008; 21: 596-601.
5. Florio P, Bruni L, Galleri L, Reis FM, Borges LE, Bocchi C, et al. Evaluation of endometrial activin A secretion for prediction of pregnancy after intrauterine insemination. Fertil Steril 2009; 93: 2316-2320. [DOI:10.1016/j.fertnstert.2008.12.125]
6. Haouzi D, Mahmoud K, Fourar M, Bendhaou K, Dechaud H, De Vos J, et al. Identification of new biomarkers of human endometrial receptivity in the natural cycle. Hum Reprod 2009; 24: 198-205. [DOI:10.1093/humrep/den360]
7. Lieberman JA, Moscicki AB, Sumerel JL, Ma Y, Scott ME. Determination of cytokine protein levels in cervical mucus samples from young women by a multiplex immunoassay method and assessment of correlates. Clin Vaccine Immunol 2008; 15: 49-54. [DOI:10.1128/CVI.00216-07]
8. Nieuwenhuizen L, Khalil MK, Venkatesh N, Othman NH. Endometrial and endocervical secretion: the search for histochemical differentiation. Anal Quant Cytol Histol 2006; 28: 87-96.
9. van der Gaast MH, Macklon NS, Beier-Hellwig K, Krusche CA, Fauser BC, Beier HM, et al. The feasibility of a less invasive method to assess endometrial maturation--comparison of simultaneously obtained uterine secretion and tissue biopsy. BJOG 2009; 116: 304-312. [DOI:10.1111/j.1471-0528.2008.02039.x]
10. Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod 2011; 26: 1270-1283. [DOI:10.1093/humrep/der037]
11. van der Gaast MH, Beier-Hellwig K, Fauser BC, Beier HM, Macklon NS. Endometrial secretion aspiration prior to embryo transfer does not reduce implantation rates. Reprod Biomed Online 2003; 7: 105-109. [DOI:10.1016/S1472-6483(10)61737-3]
12. Robertson SA, Moldenhauer LM. Immunological determinants of implantation success. Int J Dev Biol 2014; 58: 205-217. [DOI:10.1387/ijdb.140096sr]
13. Rajaei S, Zarnani AH, Jeddi-Tehrani M, Tavakoli M, Mohammadzadeh A, Dabbagh A, et al. Cytokine profile in the endometrium of normal fertile and women with repeated implantation failure. Iran J Immunol 2011;8:201-208.
14. Wegmann TG, Lin H, Guilbert L, Mosmann TR. Bidirectional cytokine interactions in the maternal-fetal relationship: is successful pregnancy a TH2 phenomenon. Immunol Today 1993; 14: 353-356. [DOI:10.1016/0167-5699(93)90235-D]
15. Chaouat G, Ledee-Bataille N, Dubanchet S, Zourbas S, Sandra O, Martal J. TH1/TH2 paradigm in pregnancy: paradigm lost? Cytokines in pregnancy/early abortion: reexamining the TH1/TH2 paradigm. Int Arch Allergy Immunol 2004; 134: 93-119. [DOI:10.1159/000074300]
16. Chaouat G. The Th1/Th2 paradigm: still important in pregnancy? Semin Immunopathol 2007; 29: 95-113. [DOI:10.1007/s00281-007-0069-0]
17. Granot I, Gnainsky Y, Dekel N. Endometrial inflammation and effect on implantation improvement and pregnancy outcome. Reproduction 2012; 144: 661-668. [DOI:10.1530/REP-12-0217]
18. Karimzadeh MA, Ayazi Rozbahani M, Tabibnejad N. Endometrial local injury improves the pregnancy rate among recurrent implantation failure patients undergoing in vitro fertilisation/intra cytoplasmic sperm injection: a randomised clinical trial. Aust N Z J Obstet Gynaecol 2009; 49: 677-680. [DOI:10.1111/j.1479-828X.2009.01076.x]
19. Zhou L, Li R, Wang R, Huang HX, Zhong K. Local injury to the endometrium in controlled ovarian hyperstimulation cycles improves implantation rates. Fertil Steril 2008; 89: 1166–1176. [DOI:10.1016/j.fertnstert.2007.05.064]
20. El-Toukhy T, Sunkara S, Khalaf Y. Local endometrial injury and IVF outcome: a systematic review and meta-analysis. Reprod Biomed Online 2012; 25: 345-354. [DOI:10.1016/j.rbmo.2012.06.012]
21. Toder V, Fein A, Carp H, Torchinsky A. TNF-alpha in pregnancy loss and embryo maldevelopment: a mediator of detrimental stimuli or a protector of the fetoplacental unit. J Assist Reprod Genet 2003; 20: 73-81. [DOI:10.1023/A:1021740108284]
22. Meisser A, Chardonnens D, Campana A, Bischof P. Effects of tumour necrosis factor-alpha, interleukin-1 alpha, macrophage colony stimulating factor and transforming growth factor on trophoblastic matrix metalloproteinases. Mol Hum Reprod 1999; 5: 252-260. [DOI:10.1093/molehr/5.3.252]
23. Cohen M, Meisser A, Haenggeli L, Bischof P. Involvement of MAPK pathway in TNF-alpha induced MMP-9 expression in human trophoblastic cells. Mol Hum Reprod 2006; 12: 225-232. [DOI:10.1093/molehr/gal023]
24. Lockwood CJ, Oner C, Uz YH, Kayisli UA, Huang SJ, Buchwalder LF, et al. Matrix metalloproteinase 9 expression in preeclamptic decidua and MMP9 induction by tumor necrosis factor alpha and interleukin 1 beta in human first trimester decidual cells. Biol Reprod 2008; 78: 1064–1072. [DOI:10.1095/biolreprod.107.063743]
25. Boomsma CM, Kavelaars A, Eijkemans MJ, Lentjes EG, Fauser BC, Heijnen CJ, et al. Endometrial secretion analysis identifies a cytokine profile predictive of pregnancy in IVF. Hum Reprod 2009; 24: 1427-1435. [DOI:10.1093/humrep/dep011]
26. Inagaki N, Stern C, McBain J, Lopata A, Kornman L, Wilkinson D. Analysis of intra-uterine cytokine concentration and matrix-metalloproteinase activity in women with recurrent failed embryo transfer. Hum Reprod 2003; 18: 608-615. [DOI:10.1093/humrep/deg139]
27. von Wolff M, Thaler CJ, Strowitzki T, Broome J, Stolz W, Tabibzadeh S. Regulated expression of cytokines in human endometrium throughout the menstrual cycle: dysregulation in habitual abortion. Mol Hum Reprod 2000; 6: 627-634. [DOI:10.1093/molehr/6.7.627]
28. Jones RL, Hannan NJ, Kaitu'u TJ, Zhang J, Salamonsen LA. Identification of chemokines important for leukocyte recruitment to the human endometrium at the times of embryo implantation and menstruation. J Clin Endocrinol Metab 2004; 89: 6155-6167. [DOI:10.1210/jc.2004-0507]
29. Inngjerdingen M, Damaj B, Mghazachi AA. Expression and regulation of chemokine receptors in human natural killer cells. Blood 2001; 97: 367-375. [DOI:10.1182/blood.V97.2.367]
30. Dominguez F, Pellicer A, Simon C. The chemokine connection: hormonal and embryonic regulation at the human maternal-embryonic interface-a review. Placenta 2003; 24: 48-55. [DOI:10.1016/S0143-4004(03)00134-6]
31. Caballero-Campo P, Domínguez F, Coloma J, Meseguer M, Remohí J, Pellicer A, et al. Hormonal and embryonic regulation of chemokines IL-8, MCP-1 and RANTES in the human endometrium during the window of implantation. Mol Hum Reprod 2002; 8: 375-384. [DOI:10.1093/molehr/8.4.375]
32. Loetscher P, Seitz M, Clark-Lewis I, Baggiolini M, Moser B. Monocyte chemotactic proteins MCP-1, MCP-2, and MCP-3 are major attractants for human CD4+ and CD8+ T lymphocytes. FASEB J 1994; 8: 1055-1060. [DOI:10.1096/fasebj.8.13.7926371]
33. Moffett A, Regan L, Braude P. Natural killer cells, miscarriage, and infertility. BMJ 2004; 329: 1283-1285. [DOI:10.1136/bmj.329.7477.1283]
34. Sela HY, Goldman-Wohl DS, Haimov-Kochman R, Greenfield C, Natanson-Yaron S, Hamani Y, et al. Human trophectoderm apposition is regulated by interferon gamma-induced protein 10 (IP-10) during early implantation. Placenta 2013; 34: 222-230. [DOI:10.1016/j.placenta.2012.12.008]
35. Nagaoka K, Nojima H, Watanabe F, Chang KT, Christenson R, Sakai S, et al. Regulation of blastocyst migration, apposition and initial adhesion by a chemokine, IFN-gamma -inducible protein 10 kDa (IP-10), during early gestation. J Biol Chem 2003; 278: 29048-29056. [DOI:10.1074/jbc.M300470200]
36. Gianaroli L, Magli MC, Ferraretti AP, Iammarrone E. Preimplantation genetic diagnosis increases the implantation rate in human in vitro fertilization by avoiding the transfer of chromosomally abnormal. Fertil Steril 1997; 68: 1128-1131. [DOI:10.1016/S0015-0282(97)00412-3]
37. Von Wolff M, Classen-Linke I, Heid D, Krusche CA, Beier-Hellwig K, Karl C, et al. Tumour necrosis factor-alpha (TNF-alpha) in human endometrium and uterine secretion: an evaluation by immunohistochemistry, ELISA and semiquantitative RT-PCR. Mol Hum Reprod 1999; 5: 146-152. [DOI:10.1093/molehr/5.2.146]
38. Rahiminejad ME, Moaddab A, Rabiee S, Esna-Ashari F, Borzouei S, Hosseini SM. The relationship between clinicobiochemical markers and depression in women with polycystic ovary syndrome. Iran J Reprod Med 2014; 12: 811-816.
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb