International Journal of
Reproductive Biomedicine
Fri, Apr 26, 2024
[Archive]
Volume 14, Issue 1 (1-2016)
IJRM 2016, 14(1): 23-28 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Saki G, Mirhoseini M, Hemadi M, Khodadadi A, Beygom Talebpour Amiri F. The effect of the melatonin on cryopreserved mouse testicular cells. IJRM 2016; 14 (1) :23-28
URL: http://ijrm.ir/article-1-689-en.html
URL: http://ijrm.ir/article-1-689-en.html
Ghasem Saki1 
, Mehri Mirhoseini * 2, Masoud Hemadi3 , Ali Khodadadi4 , Fereshteh Beygom Talebpour Amiri5
, Mehri Mirhoseini * 2, Masoud Hemadi3 , Ali Khodadadi4 , Fereshteh Beygom Talebpour Amiri5
1- Cellular and Molecular research Center, School of Medicine. Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2- Faculty of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran , m.mirhoseini@mazums.ac.ir
3- Infertility and Perinatology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4- Petroleum and Environmental Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
5- Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
2- Faculty of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran , m.mirhoseini@mazums.ac.ir
3- Infertility and Perinatology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4- Petroleum and Environmental Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
5- Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Abstract: (2877 Views)
Background: After improvements in various cancer treatments, life expectancy has been raised, but success in treatment causes loss of fertility in many of the survived young men. Cryopreservation of immature testicular tissues or cells introduced as the only way to preserve fertility. However, freezing has some harmful effects. Melatonin, a pineal gland hormone, has receptors in reproductive systems of different species. It is assumed that melatonin has free radical scavenger properties.
Objective: The aim of this study was to evaluate the effects of melatonin on the cryopreserved testicular cells in mouse.
Materials and Methods: Cells from 7- 10 days old NMRI mice testes were isolated using two step enzymatic digestion. The testicular cells were divided into two groups randomly and cryopreserved in two different freezing media with and without the addition of 100 µm melatonin. Finally, apoptosis of the cells was assayed by flow cytometry. Also, lactate dehydrogenase activity test was performed to assess the cytotoxicity.
Results: The results of lactate dehydrogenase showed the nearly cytotoxic effect of melatonin. The results of flow cytometry showed increase in apoptosis in the cryopreserved cells in the media containing melatonin compared to the control group.
Conclusion: The present study shows that melatonin has an apoptotic effect on cryopreserved mouse testicular cells.
Objective: The aim of this study was to evaluate the effects of melatonin on the cryopreserved testicular cells in mouse.
Materials and Methods: Cells from 7- 10 days old NMRI mice testes were isolated using two step enzymatic digestion. The testicular cells were divided into two groups randomly and cryopreserved in two different freezing media with and without the addition of 100 µm melatonin. Finally, apoptosis of the cells was assayed by flow cytometry. Also, lactate dehydrogenase activity test was performed to assess the cytotoxicity.
Results: The results of lactate dehydrogenase showed the nearly cytotoxic effect of melatonin. The results of flow cytometry showed increase in apoptosis in the cryopreserved cells in the media containing melatonin compared to the control group.
Conclusion: The present study shows that melatonin has an apoptotic effect on cryopreserved mouse testicular cells.
Type of Study: Original Article |
References
1. Magnani C, Pastore G, Coebergh JW, Viscomi S, Spix C, Steliarova-Foucher E. Trends in survival after childhood cancer in Europe 1978–1997: report from the Automated Childhood Cancer Information System project (AGCIS). Eur J Cancer 2006; 42: 1981-2005. [DOI:10.1016/j.ejca.2006.05.006]
2. Steliarova-Foucher E, Stiller C, Kaatsch P, Berrino F, Coebergh JW, Lacour B, et al. Geographical patterns and time trends of cancer incidence and survival among children and adolescents in Europe since the 1970s (The ACCIS project): an epidemiological study. Lancet 2004; 364: 2097-2105. [DOI:10.1016/S0140-6736(04)17550-8]
3. Brenner H, Steliarova-Foucher E, Arndt V. Up-to-date monitoring of childhood cancer long-term survival in Europe methodology and application to all forms of cancer combined. Ann Oncol 2007; 18: 1561-1568. [DOI:10.1093/annonc/mdm186]
4. Bleyer A. The impact of childhood cancer on the United States and the world. CA Cancer J Clin 1990; 40: 355-367. [DOI:10.3322/canjclin.40.6.355]
5. Keros V, Hultenby K, Borgstrom B, Fridstrom M, Jahnukainen K, Hovatta O. Methods of cryopreservation of testicular tissue with viable spermatogonia in pre-pubertal boys undergoing gonadotoxic cancer treatment. Hum Reprod 2007; 22: 1384-1395. [DOI:10.1093/humrep/del508]
6. Milazzo JP, Vaudreuil L, Cauliez B, Gruel E, Masse´ L, Mousset-Sime´on N, et al. Comparison of conditions for cryopreservation of testicular tissue from immature mice. Hum Reprod 2008; 23: 17-28. [DOI:10.1093/humrep/dem355]
7. Tournaye H, Goossens E, Verheyen G, Frederickx V, De Block G, Devroey P, et al. Preserving the reproductive potential of men and boys with cancer: current concepts and future prospects. Hum Reprod Update 2004; 10: 525-532. [DOI:10.1093/humupd/dmh038]
8. Succu S, Berlinguer F, Pasciu V, Satta V, Leoni GG, Naitana S. Melatonin protects ram spermatozoa from cryopreservation injuries in a dose-dependent manner. J Pineal Res 2011; 50: 310-318. [DOI:10.1111/j.1600-079X.2010.00843.x]
9. Sener G, Atasoy BM, Ersoy Y, Arbak S, Sengoz M, Yegen BC. Melatonin protects against ionizing radiation-induced oxidative damage in corpus cavernosum and urinary bladder in rats. J Pineal Res 2004; 37: 241-246. [DOI:10.1111/j.1600-079X.2004.00161.x]
10. Reiter RJ, Melchiorri D, Sewerynek E, Poeggeler B, Barlow-Walden L, Chuang J, et al. A review of the evidence supporting melatonin's role as an antioxidant. J Pineal Res 1995; 18: 1-11. [DOI:10.1111/j.1600-079X.1995.tb00133.x]
11. Sanchez-Hidalgo M, de la Lastra CA, Carrascosa-Salmoral MP, Naranjo MC, Gomez-Corvera A, Caballero B, et al. Age-related changes in melatonin synthesis in rat extrapineal tissues. Exp Gerontol 2009; 44: 328-334. [DOI:10.1016/j.exger.2009.02.002]
12. Guneli E, Tugyan K, Ozturk H, Gumustekin M, Cilaker S, Uysal N. Effect of melatonin on testicular damage in streptozotocin-induced diabetes rats. EurSurg Res 2008; 40: 354-360. [DOI:10.1159/000118032]
13. Bejarano I, Redondo PC, Espino J, Rosado JA, Paredes SD, Barriga C, et al. Melatonin induces mitochondrial-mediated apoptosis in human myeloid HL-60 cells. J Pineal Res 2009; 46: 392-400. [DOI:10.1111/j.1600-079X.2009.00675.x]
14. Hemadi M, Abolhassani F, Akbari M, Sobhani A, Pasbakhsh P, Ahrlund-Richter L, et al. Melatonin promotes the cumulus-oocyte complexes quality of vitrified-thawed murine ovaries; with increased mean number of follicles survival and ovary size following heterotopic transplantation. Eur J Pharmacol 2009; 618: 84-90. [DOI:10.1016/j.ejphar.2009.07.018]
15. Gholami MR, Saki G, Hemadi M, Khodadadi A, Mohammadi-Asl J. Supplementation vitrified-thawed media with melatonin do not protecting immature mouse testicular tissue from vitrified-thawed induced injury. Asian J Anim Vet Adv 2012; 7: 940-949. [DOI:10.3923/ajava.2012.940.949]
16. Milazzo JP, Vaudreuil L, Cauliez B, Gruel E, Masse L, Mousset-Simeon N, et al. Comparison of conditions for cryopreservation of testicular tissue from immature mice. Hum Reprod 2008; 23: 17-28. [DOI:10.1093/humrep/dem355]
17. Oatley JM, Reeves JJ, McLean DJ. Biological activity of cryopreserved bovine spermatogonial stem cells during in vitro culture. Biol Reprod 2004; 71: 942-947. [DOI:10.1095/biolreprod.104.028894]
18. Shirazi A, Ghobadi G, Ghazi-Khansari M. A radiobiological review on melatonin: a novel radioprotector. J Radiat Res 2007; 48: 263-272. [DOI:10.1269/jrr.06070]
19. Karbownik M, Reiter RJ. Antioxidative effects of melatonin in protection against cellular damage caused by ionizing radiation. Proc Soc Exp Biol Med 2000; 225: 9-22. [DOI:10.1046/j.1525-1373.2000.22502.x]
20. Nasiraei-Moghadam SN, Parivar K, Ahmadiani A, Movahhedin M, Vaez Mahdavi MR. Protective effect of melatonin against inequality-induced damages on testicular tissue and sperm parameters. Int J Fertil Steril 2014; 7: 313-322.
21. Lissoni P, Tancini G, Barni S, Paolorossi F, Ardizzoia A, Conti A, et al. Treatment of cancer chemotherapy-induced toxicity with the pineal hormone melatonin. Support Care Cancer 1997; 5: 126-129. [DOI:10.1007/BF01262569]
22. Sewerynek E, Reiter RJ, Melchiorri D, Ortiz GG, Lewinski A. Oxidative damage in the liver induced by ischemia-reperfusion: protection by melatonin. Hepatogastroenterology 1996; 43: 898-905.
23. Korzeniewski C, Callewaert DM. An enzyme-release assay for natural cytotoxicity. J Immunol Methods 1983; 64: 313-320. [DOI:10.1016/0022-1759(83)90438-6]
24. Weyermann J, Lochmann D, Zimmer A. A practical note on the use of cytotoxicity assays. Int J Pharm 2005; 288: 369-376. [DOI:10.1016/j.ijpharm.2004.09.018]
25. Mohamad-Ghasemi F, Faghani M, Khajehjahromi S, Bahadori M, Nasiri E, Hemadi M. Effect of melatonin on proliferative activity and apoptosis in spermatogenic cells in mouse under chemotherapy. J Reprod Contracep 2010; 21: 79-94. [DOI:10.1016/S1001-7844(10)60016-8]
26. Mirhoseini M, Saki G, Hemadi M, Khodadadi A, Mohammadi- Asl J. Melatonin and testicular damage in busulfan treated Mice. Iran Red Crescent Med J 2014; 16: e14463. [DOI:10.5812/ircmj.14463]
27. Succu S, Berlinguer F, Pasciu V, Satta V, Leoni GG, Naitana S. Melatonin protects ram spermatozoa from cryopreservation injuries in a dose-dependent manner. J Pineal Res 2011; 50: 310-318. [DOI:10.1111/j.1600-079X.2010.00843.x]
28. Yoshikazu Y, Atsuhiko H, Kazuyoshi T, Masako O, Bunpei I. Effects of Melatonin: Basics Studies and Clinical Applications. Anti-aging Medicin 2010; 7: 85-91.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
