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Volume 14, Issue 10 (10-2016)
IJRM 2016, 14(10): 629-636 |
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Nazanin S, Mohammadghasemi F, Ebrahimi H, Rafati Sajedi H, Chatrnour G. Ovarian and uterine alterations following forced swimming: An immunohistochemical study. IJRM 2016; 14 (10) :629-636
URL: http://ijrm.ir/article-1-698-en.html
URL: http://ijrm.ir/article-1-698-en.html
Seyedeh Nazanin1 
, Fahimeh Mohammadghasemi * 2, Hannan Ebrahimi1 , Hanieh Rafati Sajedi1 , Gelayol Chatrnour1
, Fahimeh Mohammadghasemi * 2, Hannan Ebrahimi1 , Hanieh Rafati Sajedi1 , Gelayol Chatrnour1
1- Student Research Center, Guilan University of Medical Sciences, Rasht, Iran
2- Anatomy Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran ,parsahistolab@gmail.com
2- Anatomy Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran ,
Abstract: (5093 Views)
Background: Physical exercise is known to be a stressor stimulus that leads to reproductive disruption.
Objective: The aim of this study was to evaluate the effect of forced swimming on the uterus and ovaries in mice.
Materials and Methods: Adult mice (N=24) were divided into the following threegroups: A, control; B, swimming in water (10PoPC); and C, swimming in water (23PoPC).Swimmers swam for 5 min daily for 5 consecutive days/ wk during 2 wks. Anenzyme linked immunosorbent assay was used to determine serum estradiol, folliclestimulating hormone (FSH) and testosterone levels. Immunohistochemistry wasperformed to study apoptotic cells or estrogen receptor (ER) expression in uterineepithelial cells and ovaries. ANOVA was used for statistical analysis56T.
Results: Swimming in both groups reduced the serum FSH and estradiol levels (p<0.01) without having a significant effect on the serum testosterone level or percentage of apoptosis in ovarian and uterine tissues (p<0.01) compared with controls. A significant reduction in the number of ERs in the uterus and ovaries, and secondary and graafian follicles were observed in groups B and C compared with controls (p<0.01); however the number of primordial and primary follicles were not significantly changed in the ovaries.
Conclusion: Forced swimming of 2 wks duration reduces the serum levels of FSH and estradiol without having effects on apoptosis in the ovaries or uteri of mice. Over a long period of time, forced swimming may have an adverse effect on fertility.
Objective: The aim of this study was to evaluate the effect of forced swimming on the uterus and ovaries in mice.
Materials and Methods: Adult mice (N=24) were divided into the following threegroups: A, control; B, swimming in water (10PoPC); and C, swimming in water (23PoPC).Swimmers swam for 5 min daily for 5 consecutive days/ wk during 2 wks. Anenzyme linked immunosorbent assay was used to determine serum estradiol, folliclestimulating hormone (FSH) and testosterone levels. Immunohistochemistry wasperformed to study apoptotic cells or estrogen receptor (ER) expression in uterineepithelial cells and ovaries. ANOVA was used for statistical analysis56T.
Results: Swimming in both groups reduced the serum FSH and estradiol levels (p<0.01) without having a significant effect on the serum testosterone level or percentage of apoptosis in ovarian and uterine tissues (p<0.01) compared with controls. A significant reduction in the number of ERs in the uterus and ovaries, and secondary and graafian follicles were observed in groups B and C compared with controls (p<0.01); however the number of primordial and primary follicles were not significantly changed in the ovaries.
Conclusion: Forced swimming of 2 wks duration reduces the serum levels of FSH and estradiol without having effects on apoptosis in the ovaries or uteri of mice. Over a long period of time, forced swimming may have an adverse effect on fertility.
Type of Study: Original Article |
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