شنبه 8 دی 1403
[Archive]
دوره 5، شماره 3 - ( 4-1386 )
جلد 5 شماره 3 صفحات 0-45 |
برگشت به فهرست نسخه ها
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Bakhtiari M, Sobhani A, Akbari M, Pasbakhsh P, Abbasi M, Hedayatpoor A, et al . The effect of hyaluronic acid on motility, vitality and fertilization capability of mouse sperms after cryopreservation. IJRM 2007; 5 (3) :45-0
URL: http://ijrm.ir/article-1-71-fa.html
URL: http://ijrm.ir/article-1-71-fa.html
The effect of hyaluronic acid on motility, vitality and fertilization capability of mouse sperms after cryopreservation. International Journal of Reproductive BioMedicine. 1386; 5 (3) :45-0
چکیده: (2938 مشاهده)
Background: Various approaches have been used in the attempts to improve the quality of frozen–thawed mouse sperms. According to literatures, it seems that hyaluronic acid (HA) has an important role on the permeability and motility of sperms and their interaction with gametes.
Objective: For evaluation of HA supplementation on sperm characteristics and fertilization capability, we investigated the effect of different doses of HA on mouse sperm morphology, motility, vitality and fertilization capability after freezing and thawing.
Materials and Methods: The cauda epididymes was removed from 6 male mice with aseptic method. The sperm samples were frozen in 1.8 ml cryotubes with 18% raffinose and 3% skimmed milk containing cryo-protectant solution. HA at the concentration of 750, 1000 or 1250 µg/ml was supplemented to frozen-thawed sperms. Sperm motility was measured with microscope, and fertilization rate was evaluated after routine IVF by counting the fertilized oocytes. For sperm morphology, papaniclau staining was used while; Eosin B was used for the assessment of sperm viability rate.
Results: HA supplementation (750 µg/ml) improved motility parameters (p < 0.05) and increased the fertility rate (p < 0.05). The effect of 1,000 µg/ml HA was also positive on the sperms. But 1,250 µg/ml HA had negative effect on above mentioned characteristic. On the other hand, none of these doses had any effect on sperm morphology.
Conclusion: The dose of 750 µg/ml of HA has the greatest effect on the motility, vitality and fertility rate of sperms after cryopreservation.
Objective: For evaluation of HA supplementation on sperm characteristics and fertilization capability, we investigated the effect of different doses of HA on mouse sperm morphology, motility, vitality and fertilization capability after freezing and thawing.
Materials and Methods: The cauda epididymes was removed from 6 male mice with aseptic method. The sperm samples were frozen in 1.8 ml cryotubes with 18% raffinose and 3% skimmed milk containing cryo-protectant solution. HA at the concentration of 750, 1000 or 1250 µg/ml was supplemented to frozen-thawed sperms. Sperm motility was measured with microscope, and fertilization rate was evaluated after routine IVF by counting the fertilized oocytes. For sperm morphology, papaniclau staining was used while; Eosin B was used for the assessment of sperm viability rate.
Results: HA supplementation (750 µg/ml) improved motility parameters (p < 0.05) and increased the fertility rate (p < 0.05). The effect of 1,000 µg/ml HA was also positive on the sperms. But 1,250 µg/ml HA had negative effect on above mentioned characteristic. On the other hand, none of these doses had any effect on sperm morphology.
Conclusion: The dose of 750 µg/ml of HA has the greatest effect on the motility, vitality and fertility rate of sperms after cryopreservation.
نوع مطالعه: Original Article |
فهرست منابع
1. Jaenisch R. Transgenic animals. Science 1988; 240: 1468-1473. [DOI:10.1126/science.3287623]
2. Bedell MA, Largaespada DA, Jenkins NA , Copeland NG. Mouse models of human disease. Part II: Recent progress and future directions. Genes Dev 1997; 11: 11-43. [DOI:10.1101/gad.11.1.11]
3. Simpson EM, Linder CC, Sargent EE, Davisson MT, Mobraaten LE. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat Genet 1997; 16: 19-27. [DOI:10.1038/ng0597-19]
4. Hrabe de Angelis M , Balling R. Large Scale ENU screens in the mouse: genetics meets genomics. Mutat Res 1998; 400: 25-32. [DOI:10.1016/S0027-5107(98)00061-X]
5. Brown SDM , Nolan PM. Mouse mutagenesis-systematic studies of mammalian gene function. Hum Mol Genet 1998; 7: 1627-1633. [DOI:10.1093/hmg/7.10.1627]
6. Pomeroy KO. Cryopreservation of transgenic mice. GATA (Genet Anal Tech Appl) 1991; 8: 95-101. [DOI:10.1016/1050-3862(91)90043-Q]
7. Mobraaten L. The Jackson laboratory genetics stocks resource repository. In Frozen Storage of Laboratory Animals, GH Zeilmaker, ed. Stuttgart, NY: (Gustav Fischer Verlag) 1981; 165-177.
8. Nakagata N. Use of cryopreservation techniques of embryos and spermatozoa for production of transgenic (Tg) mice and for maintenance of Tg mouse lines. Lab Anim Sci 1996; 46: 236-238.
9. Nakagata N. Cryopreservation of mouse spermatozoa. Mammalian Genome. 2000; 11: 572-576. [DOI:10.1007/s003350010109]
10. Mazur P. Freezing of living cells: mechanisms and implications. Am J Physiol Cell Physiol 1984; 16: 125-42. [DOI:10.1152/ajpcell.1984.247.3.C125]
11. Eriksson BM, Vazquez JM, Martinez E, Roca J, Lucas X, Rodríguez-Martinez H. Effect of holding time during cooling and of type of package on plasma membrane integrity, motility and in vitro oocyte penetration ability of frozen-thawed boar spermatozoa. Theriogenology 2001; 55: 1593-1605. [DOI:10.1016/S0093-691X(01)00505-2]
12. Zeng WX , Terada T. Protection of boar spermatozoa from cold shock damage by 2-hydroxypropyl-beta-cyclodextrin. Theriogenology 2001; 55: 615-627. [DOI:10.1016/S0093-691X(01)00430-7]
13. Zeng WX , Terada T. Effects of methyl-beta-cyclodextrins on cryosurvival of boar spermatozoa. J Androl 2001; 22: 111-118.
14. Erlinger R. Glycosaminoglycans in porcine lung: an ultrastructural study using cupromeromic blue. Cell Tissue Res 1995; 281: 473-483. [DOI:10.1007/BF00417864]
15. Vines CA, Li MW, Deng X, Yudin AI, Cherr GN ,Overstreet JW. Identification of hyaluronic acid (HA) binding domain in the PH-20 protein that may function in cell signaling. Mol Reprod Dev 2001; 60: 542-545. [DOI:10.1002/mrd.1119]
16. Ghosh I, Bharadwaj A , Datta K. Reduction in the level of hyaluronan binding protein (HABP1) is associated with loss of sperm motility. J Reprod Immunol 2002; 53: 45-54. [DOI:10.1016/S0165-0378(01)00095-X]
17. Suzuki K, Eriksson B, Shimizu H, Nagai T ,Rodriguez-Martinez H. Effect of hyaluronan on monospermic penetration of porcine oocytes. Int J Androl 2000; 23: 13-21. [DOI:10.1046/j.1365-2605.2000.t01-1-00198.x]
18. Suzuki K, Asano A, Eriksson B, Niwa K, Nagai T, Rodriguez Martinez H. Capacitation status and in vitro fertility of boar spermatozoa: effects of seminal plasma. Int J Androl 2002; 25: 84-93. [DOI:10.1046/j.1365-2605.2002.00330.x]
19. Huszar G, Willetts M, Corrales M. Hyaluronic acid (Sperm Select) improves retention of sperm motility and velocity in normospermic and oligospermic specimens. Fertil Steril 1990; 54: 1127-1134. [DOI:10.1016/S0015-0282(16)54016-3]
20. Sbracia M, Grasso J, Sayme N, Stronk J , Huszar G. Hyaluronic acid substantially increases the retention of motility in cryopreserved thawed human spermatozoa. Hum Reprod 1997; 12: 1949-1954. [DOI:10.1093/humrep/12.9.1949]
21. Fuller SJ, Whittingham DG. Effect of cooling mouse spermatozoa to 4°C on fertilization and embryonic development. J Reprod Fertil 1996; 108: 139-45. [DOI:10.1530/jrf.0.1080139]
22. Penfold LM, Moore HD. A New Method for cryopreservation of mouse spermatozoa. J Reprod Fertil 1993; 99: 131-134. [DOI:10.1530/jrf.0.0990131]
23. Nakagata N , Takeshima T. High fertilizing ability of mouse spermatozoa diluted slowly after cryopreservation. Theriogenology 1992; 37: 1283-1291. [DOI:10.1016/0093-691X(92)90183-R]
24. Songsasen N, Leibo SP. Cryopreservation of mouse spermatozoa. I. Effect of seeding on fertilizing ability of cryopreserved spermatozoa.Cryobiology 1997; 35: 240-54. [DOI:10.1006/cryo.1997.2048]
25. Mazur P, Cryobiology. The freezing of biological systems. Science 1970; 168: 939-949. [DOI:10.1126/science.168.3934.939]
26. Holt WV. Basic aspects of frozen storage of semen. Anim Reprod Sci 2000; 662, 3-22. 27. Sztein JM, Schmidt PM, Raber J, Rall WF. Cryopreservation of mouse spermatozoa in a glycerol raffinose solution. Cryobiology 1992; 29: 736-737.
27. Rodríguez-Martinez H, Tienthai P, Suzuki K, Funahashi H, Ekwall H, Johannisson A. Involvement of oviduct in sperm capacitacion and oocyte development in pigs. Reprod Suppl 2001; 58: 129-145.
28. Huszar G, Ozkavukcu S, Jakab A, Celik-Ozenci C, Sati GL, Cayli S. Hyaluronic acid binding ability of human sperm reflects cellular maturity and fertilizing potential: selection of sperm for intracytoplasmic sperm injection. Curr Opin Obstet Gynecol 2006; 18:260-7. [DOI:10.1097/01.gco.0000193018.98061.2f]
29. Kornovsky BS, McCoshen JM, Krendenter J , Turley E.The regulation of sperm motility by a novel hyaluronan receptor. Fertil. Steril 1994; 61: 935-940. [DOI:10.1016/S0015-0282(16)56709-0]
30. Ranghanatan S, Ganguly A.K , Datta K. Evidence for presence of hyaluronan binding protein on spermatozoa and its possible involvement in sperm function. Mol Reprod Dev 1994; 38: 69-76. [DOI:10.1002/mrd.1080380112]
31. Ranghanatan S, Bharadwaj A, Datta K. Hyaluronan mediates sperm motility by enhancing phosphorilation of proteins including hyaluronan binding protein. Cell Mol Biol Res 1995; 41: 467-476.
32. Prevo R, Banerji S, Ferguson D, Clasper S, Jackson DG. Mouse LYVE-1 is an endocytic receptor for hyaluronan in lymphatic endothelium. J Biol Chem 2001; 276: 19420-19430. [DOI:10.1074/jbc.M011004200]
33. Tienthai P, Kjellén L, Pertoft H, Suzuki K ,Rodriguez- Martinez H. Localisation and quantitation of hyaluronan and sulphated glycosaminoglycans in the tissues and intraluminal fluid of the pig oviduct. Reprod Fertil Dev 2001; 12: 173-182. [DOI:10.1071/RD00034]
34. Rodriguez-Martinez H. Oviduct function in cows and pigs: with special reference to sperm capacitation. Asian-Aust J Anim Sci 2001; 14: 28-37.
35. Rodriguez-Martinez H. The oviduct of the pig: do intraluminal glycosaminoglycans play a role in tubal function? In: Johnson LA, Guthrie HD, editors. Boar semen preservation IV. Lawrence, KS, USA: Allen Press Inc 2000; 153-63.
36. Huszar G, Willetts M, Corrales M. Hyaluronic acid (Sperm Select) improves retention of sperm motility and velocity in normospermic and oligospermic specimens. Fertil Steril 1990; 54: 1127-1134. [DOI:10.1016/S0015-0282(16)54016-3]
37. Kornovski B.S, McCoshen J, Kredentser J, Turley E. The regulation of sperm motility by a novel hyaluronan receptor. Fertil Steril 1994; 61: 935-940. [DOI:10.1016/S0015-0282(16)56709-0]
38. Ranganathan S, Ganguly A.K, Datta K. Evidence for presence of hyaluronan binding protein on spermatozoa and its possible involvement in sperm function. Mol Reprod Dev 1994; 38: 69-76. [DOI:10.1002/mrd.1080380112]
39. Bharadwaj A, Ghosh I, Sengupta A, Cooper TG, Weinbauer GF, Brinkworth MH ,et al. Stage-specific expression of proprotein form of hyaluronan binding protein 1 (HABP1) during spermatogenesis in rat. Mol Reprod Dev 2002; 62: 223-232. [DOI:10.1002/mrd.10135]
40. Gabor Huszar, Ciler Celik Ozenci, Sevil Cayli, Zoltan Zavaczki M.D, Eleonora Hansch , Lynne Vigue. Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status. Fertil Steril 2003; 79:1616-1624. [DOI:10.1016/S0015-0282(03)00402-3]
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
